Study on effect and mechanism of total flavonoids of Hippophae in inhibiting proliferation and migration of lung cancer A549 cellsApr. 6,2021
Flavonoids are one of the most important active components in Hippophae small trees or shrubs (Elaeagnaceae), particularly the quercetin, isorhamnetin, and kaempferol. They have anti-tumor, anti-inflammatory, heart and cerebral vessels-protecting, and immunity-improving functions. Lung cancer is one of the most common cancers in the world, with a high incidence and fatality rate. The high fatality is mainly caused by the metastasis and the subsequent resistance to chemoradiotherapy after the first diagnosis. In recent years, the anti-tumor effect of total flavonoids of Hippophae (TFH) has been gradually recognized and the intestinal absorption properties of TFH are better than the single flavonoid isorhamnetin. Flavonoids of Hippophae have inhibitory effects on many tumor cells, such as colon HT29 cancer cells, human prostate cancer PC-3 cells, and human breast cancer cell line Bcap-37. Flavonoids enhance the sensitivity of non-small cell lung carcinoma to some chemotherapeutic drugs such as cisplatin.
JIA et al. from College of Life Science, Northwest Normal University pretreated the lung cancer A549 cells with different concentrations of total flavonoids from Hippophae tibetana (TFHt), H. rhamnoides subsp. sinensis (TFHr), and H. neurocarpa (TFHn). Relative cell viability, colony formation ability, and apoptosis rate were detected to investigate the inhibition effect of TFH on A549 proliferation. Furthermore, the effect of TFHt on the invasion and migration of lung cancer cells was further analyzed, and the related molecular mechanisms were discussed. The result is expected to provide a theoretical basis for the efficient development and utilization of Hippophae resources.
The results showed that the total flavonoids from three species of Hippophae could inhibit the growth of A549 cells and the inhibition effect was in the order of TFHt, TFHr, and TFHn. TFHt had obvious advantages in inhibiting the proliferation and reducing the invasion and migration ability of lung cancer A549 cells, which may be related to the down-regulation of the expression of MMP9 by TGF-β and the inhibition of EMT of lung cancer.
The related research was completed by JIA Cong, DU Ya-rong, SUN Kun, etc. Results are published in the 32nd volume, 6th issue of Natural Product Research and Development, 2020. The bilingual version has been launched.
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