Study on the Difference between Xuefu Zhuyu Capsule and Vascular Endothelial Growth Factor in Promoting Angiogenesis
Chinese Journal of Integrated Traditional and Western Medicine,2018,Vol 38,No. 04
Objective To observe the differences in angiogenesis between Xuefu Zhuyu Capsule and vascular endothelial growth factor (VEGF) in vitro and their respective effects on gene expression profiles, and provide a molecular regulatory model for exploring the differences in angiogenesis behavior between Chinese and western drugs. Methods Through the analysis of the results of Xuefu Zhuyu Capsule-containing serum and VEGF affecting in vitro angiogenesis of endothelial cell HMEC-1, the behavior differences between the two were clarified, and the best drug conditions for promoting angiogenesis were determined. Gene expression profiling (DGE) technology was used to analyze the effects of two drugs on gene expression under optimal pharmacodynamic conditions. Results Compared with the blank serum group, the number of vascular lumens in the 2.5% of Xuefu Zhuyu Capsule-containing serum group was increased significantly after 48-h treatment, while the numbers of vascular lumens in the 5.0% of Xuefu Zhuyu Capsule-containing serum group were decreased significantly after 48 h and 72 h of treatment (both P < 0.01). Compared with the blank group, the numbers of vascular lumens in the 1.25, 2.5, 5 and 10 ng/mL of VEGF groups were increased significantly ( P < 0.01). Therefore, the 2.5% of Xuefu Zhuyu Capsule-containing serum and 10 ng/mL of VEGF for 48 h were considered as the optimal conditions for promoting angiogenesis. Further DGE results showed that seven genes (SCGB3 A1, FTPA2, IGLL5, SFTPC, SFTPA1, CD74, and SFTPB) were differentially expressed genes affected by VEGF. They were all down-regulated and involved in whooping cough, cytophagy, primary immunodeficiency, antigen processing and presentation, lysosome, herpes simplex infection and pulmonary tuberculosis pathways. Five genes (OTX1, BCYRN1, MR-PL50, CCDC104 and TCEA L1) were differentially expressed genes affected by Xuefu Zhuyu Capsule. OTX1 was up-regulated while the other 4 were down-regulated, involving no pathway. Conclusions Xuefu Zhuyu Capsule could promote angiogenesis and bi-directionally control vessel growth, but VEGF only promoted vessel growth. The different behaviors in promoting angiogenesis of those two drugs were closely related to the genes affected by them.
Therapeutic Analysis of Qingchang Wenzhong Formula for Treatment of Mild-Moderate Ulcerative Colitis Patients
Chinese Journal of Integrated Traditional and Western Medicine,2018,Vol 38,No. 01
Objective To observe the effects of Qingchang Wenzhong Formula (QWF) on mild-moderate ulcerative colitis (UC) patients with syndrome of cold and heat in complexity and damp-heat stasis and obstruction. Methods Totally 72 mild-moderate UC patients were assigned to the treatment group and the control group according to random number table, 36 cases in either group. Patients in the treatment group took QWF and Mesalazin Enteric-coated Tablets, while patients in the control group took Mesalazin Enteric-coated Tablets and QWF mimetics. The treatment course for all was eight weeks. Before and after treatment, scores of single symptom, Baron, improved Mayo score were used to evaluate therapeutic effects. Clinical symptoms of Chinese medicine were observed to evaluate Chinese medicine clinical efficacy. Inflammatory Bowel Disease Questionnaire (IBDQ) was also used to judge life quality under the treatments. Results Sixty-one cases were enrolled by eliminating unqualified cases (29 cases in treatment group and 32 cases in control group). Scores of single symptom (abdominal pain, diarrhea, purulent bloody stool), Chinese medicine syndrome, Baron, improved Mayo score, and IBDQ in the treatment group were improved in the two groups after treatment as compared with those before treatment ( P < 0.01). All indices showed no difference between two groups after treatment ( P > 0.05). Conclusion QWF could effectively ameliorate clinical symptoms, reduce disease activity, and improve life quality of mild-moderate UC patients in clinical practice.
Effects of Erzhi Tiangui and Qigong Compound on the AKT-GLUT4 Insulin Signal Pathway of Polycystic Ovarian Syndrome and IVF-ET Outcomes
Chinese Journal of Integrated Traditional and Western Medicine,2018,Vol 38,No. 04
Objective To investigate the effects of Erzhi Tiangui and Qigong Compound (ETAQC) on AKT-GLUT4 insulin signal pathway inside ovaries and its effect on pregnancy outcomes of infertile patients with polycystic ovary syndrome (PCOS). Methods A total of 66 PCOS patients with kideny deficiency and phlegm-dampness treated by in vitro fertilization-embryo transfer (IVF-ET) were randomly assigned to two groups (33 patients per group): ETAQC group (Chinese medicine group) and placebo group. Patients of two groups were treated with double-drop method (GnRH-a + Diane-35). Patients of ETAQC group took Diane-35 while taking ETAQC until HCG injection day. Patients in the placebo group were given placebo granules as a contrast. Score changes of kidney deficiency and phlegm-dampness syndrome before and after treatment were measured. Other outcomes including HOMA-IR, high-quality oocyte rate, high-quality embryo rate, clinical pregnancy rate, and the expression of AKT, p-AKT, and GLUT4 in ovarian granular cells on the day of oocyte retrieval were measured. Results The syndrome score and HOMA-IR decreased significantly after treatment in ETAQC group compared with those in the placebo group ( P < 0.01). In addition, ETAQC group showed significant increase in the oocyte number, fertilization rate, high-quality embryo rate, and clinical pregnancy rate ( P < 0.05, P < 0.01). Expression of p-AKT and GLUT4 in granular cells of ETAQC group was increased compared with that in the placebo group ( P < 0.05). The dosage and utilizaiton days of Gn and expression of AKT protein showed no significant difference between the two groups. Conclusion ETAQC could up-regulate the expression of p-AKT and GLUT4 in ovarian granular cells and thereby remarkably ameliorate the insulin resistance degree in the ovary tissue, so as to improve the quality of ootid and the outcome of IVF-ET patients with PCOS.
Efficacy Observation of Fuzheng Huayu Capsule Combined with Entecavir in the Treatment of Hepatitis B Cirrhosis
Chinese Journal of Integrated Traditional and Western Medicine,2018,Vol 38,No. 12
Objective To observe the efficacy and safety of Fuzheng Huayu Capsule (FHC) combined with entecavir (ETV) in the treatment of hepatitis B cirrhosis. Methods Prospective case-control study was used. Totally 155 patients with hepatitis B cirrhosis were assigned to the control group (77 cases) and the treatment group (78 cases) by random number table. Patients in the control group were treated with ETV, while those in the treatment group additionally took FHC. The total effective rate of the two groups was observed after 48 weeks and 72 weeks of treatment. The changes of liver function indices, HBV-DNA, hepatic fibrosis indices (hyaluronic acid, type Ⅲ procollagen, type Ⅳ collagen and laminin), liver stiffness measurement (LSM), and three ultrasound imaging indices (internal diameter of the portal vein, spleen length and spleen thickness) were compared. Results After 48 weeks and 72 weeks of treatment, the total effective rate of the treatment group was 87.18% (68/78) and 92.31% (72/78), higher than 71.43% (55/77) and 77.92% (60/77) of the control group, with statistical significance ( χ2 = 5.868, 6.346, P < 0.05). There was no statistical difference in ALT/AST and TBIL normalization rate and HBV-DNA negative conversion rate between the two groups at week 48 ( P > 0.05). They were higher in the treatment group than in the control group at week 72 ( P < 0.05). Four items of liver fibrosis: The indices of both groups dropped significantly after 48 weeks and 72 weeks of treatment; for each group, the indices after 72 weeks of treatment were lower than those after 48 weeks ( P < 0.05); the indices of the treatment group after 72 weeks were lower than those of the control group ( P < 0.05). Liver fibrosis value, internal diameter of the portal vein, spleen length and spleen thickness: They decreased more obviously in the treatment group at week 48 and 72 and in the control group at week 72, as compared with those before treatment in the same group ( P < 0.05); they showed a significant decrease in the treatment group at week 72, as compared with those at week 48 and those in the control group at week 72 ( P < 0.05). No adverse reaction occurred in the two groups. Conclusion The effect and safety of FHC combined with ETV in the treatment of hepatitis B cirrhosis were obviously better than those of ETV alone.
Chinese Journal of Plant Ecology,2018,Vol 42,No. 11
The research on the ecological processes in rhizosphere and the underlying mechanisms has become one of the active and sensitive hotspots in soil science. Root exudates have special roles in mediating the nutrient cycling and signal transduction within root–soil–microorganism interactions. They are the key driving factors regulating the functions of rhizosphere micro-ecosystem, and serve as a major premise for the concept and ecological processes in rhizosphere. However, due to the instinctive advantages of crops, such as short life cycles and convenient operation, the available studies on root exudates mainly focus on agricultural ecosystems and target at providing practical guidelines. In contrast, there have been few investigations on root exudates of trees, which highly limited the knowledge of the potential mechanisms of root exudates in mediating soil biogeochemical processes in forest ecosystems. Based on the main findings in our previous studies and the emerging frontiers in rhizosphere ecology, we specifically reviewed the ecological consequences and key challenges in the research on root exudates in forests. Finally, we put forward several topics and research outlooks for guiding future work to facilitate studies on root exudates and their ecological consequences in forest ecosystems.