Effect of Panaxatriol Saponin Enzymatic Hydrolysates from Panax ginseng Stem and Leaves on the Treatment of Alzheimer’s Mice Induced by Alchlor/D-Galactose
(2.Key Laboratory of Effective Components Analysis of Changbai Mountain Chinese Medicinal Materials, Changchun, China 130118)
(3.College of Food Science, Jilin Agricultural University, Changchun, China 130118)
【Abstract】 Objective: To observe the therapeutical effect of panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves (GSLPSH) on the treatment of Alzheimer’s disease (AD). Methods: The AD model was established by administration of alchlor combined with D-galactose. The changes in learning and memory abilities of mice were measured by step-down test. The activities of acetylcholinesterase (AChE), glutathione peroxidase (GSH-Px), as well as phospho-Tau (P-Tau) and acetylcholine (ACH) content in mouse hippocampus, were detected by ELASA. The expressions of Tau protein, cyclin-dependent-like kinase 5 (CDK5), microtubule-associated protein 2 (MAP2), nuclear factor NF-kappa-B p65 (NF-κB p65), and amyloid beta (Aβ) proteins in mouse hippocampus were observed by immunohistochemistry. Results: GSLPSH could improve the learning and memory ability, reduce the levels of AChE and P-Tau, increase the levels of GSH-Px and ACH, inhibit the expressions of Tau, CDK5, NF-κB p65, and Aβ proteins, and promote the MAP2 protein expression in the hippocampus of AD mice induced by alchlor/D-galactose. Conclusion: GSLPSH has therapeutic effect on AD model mice induced by alchlor/D-galactose. It improves symptoms in AD mice by inhibiting the deposition of Aβ protein and the excessive phosphorylation of Tau protein, enhancing the activity of GSH-Px, and reducing the expression of inflammatory factor NF-κB p65.
【Keywords】 Panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves; Alchlor/D-galactose; Alzheimer’s disease;
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