Effect of Panaxatriol Saponin Enzymatic Hydrolysates from Panax ginseng Stem and Leaves on the Treatment of Alzheimer’s Mice Induced by Alchlor/D-Galactose

MA He-tong1 ZHU Hong-yan1 GAO Yu-gang1 HE Zhong-mei1,2 YANG He1 ZHAO Yan1 BI Yun-feng3

(1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China 130118)
(2.Key Laboratory of Effective Components Analysis of Changbai Mountain Chinese Medicinal Materials, Changchun, China 130118)
(3.College of Food Science, Jilin Agricultural University, Changchun, China 130118)

【Abstract】 Objective: To observe the therapeutical effect of panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves (GSLPSH) on the treatment of Alzheimer’s disease (AD). Methods: The AD model was established by administration of alchlor combined with D-galactose. The changes in learning and memory abilities of mice were measured by step-down test. The activities of acetylcholinesterase (AChE), glutathione peroxidase (GSH-Px), as well as phospho-Tau (P-Tau) and acetylcholine (ACH) content in mouse hippocampus, were detected by ELASA. The expressions of Tau protein, cyclin-dependent-like kinase 5 (CDK5), microtubule-associated protein 2 (MAP2), nuclear factor NF-kappa-B p65 (NF-κB p65), and amyloid beta (Aβ) proteins in mouse hippocampus were observed by immunohistochemistry. Results: GSLPSH could improve the learning and memory ability, reduce the levels of AChE and P-Tau, increase the levels of GSH-Px and ACH, inhibit the expressions of Tau, CDK5, NF-κB p65, and Aβ proteins, and promote the MAP2 protein expression in the hippocampus of AD mice induced by alchlor/D-galactose. Conclusion: GSLPSH has therapeutic effect on AD model mice induced by alchlor/D-galactose. It improves symptoms in AD mice by inhibiting the deposition of Aβ protein and the excessive phosphorylation of Tau protein, enhancing the activity of GSH-Px, and reducing the expression of inflammatory factor NF-κB p65.

【Keywords】 Panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves; Alchlor/D-galactose; Alzheimer’s disease;


【Funds】 Soft Science Research Program of Jilin Province (20180414072GH)

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    [1] Yin YF, Tong YG, Qing H. Progress in the treatment of Alzheimer’s disease [J]. Letters in Biotechnology, 2013 (2): 276–279 (in Chinese).

    [2] Wu L, Chen YB, Wang Q, et al. In-vitro inhibitory effect of ginsenoside Rg1 on Aβ25–35-induced NG108–15 apoptosis in Alzheimer’s disease cellular model [J]. Journal of Guangzhou University of Traditional Chinese Medicine, 2007, 24: 126–131 (in Chinese).

    [3] Yang B. Biotransformation of panoxatriol type ginsenosides [D]. Master’s thesis at Dalian Polytechnic University, 2009 (in Chinese).

    [4] Masters CL, Simms G, Weinman NA, et al. Amyloid plaque core protein in Alzheimer disease and Down syndrome [J]. Proc Natl Acad Sci USA, 1985, 82: 4245–4249.

    [5] De Strooper B, Annaert W. Proteolytic processing and cell biological functions of the amyloid precursor protein [J]. J Cell Sci, 2000, 113: 1857–1870.

    [6] Soreq H, Seidman S. Acetylcholinesterase—new roles for an old actor [J]. Nat Rev Neurosci, 2001, 2: 294–302.

    [7] Hampel H, Blennow K, Shaw LM, et al. Total and phosphorylated tau protein as biological markers of Alzheimer’s disease [J]. Exp Gerontol, 2010; 45 (1): 30–40.

    [8] Plouffe V, Mohamed NV, Rivest-McGraw J, et al. Hyperphosphorylation and cleavage at D421 enhance tau secretion [J]. PLoS One, 2012, 7 (5): e36873.

    [9] Li L, Zhong WH, Gao XL. 浅谈老年性痴呆症致病机理与自由基的相关性 [J]. Seek Medical and Ask the Medicine, 2012, 10 (10): 453–453 (in Chinese).

    [10] Ma S. Progress on GSH-Px and GST [J]. Progress in Veterinary Medicine, 2008, 29 (10): 53–56.

    [11] Williams RO, Paleolog E, Feldmann M. Cytokine inhibitors in rheumatoid arthritis and other autoimmune diseases [J]. Current Opinion Pharmacology, 2007, 7 (4): 412–417.

    [12] Zhou JC. The Role of Cdk5 in neuronal death and synaptic dysfunctions through phosphorylating FOXO1 and BAG3 [D]. doctoral dissertation of Xiamen University, 2016 (in Chinese).

    [13] Liang C. The study on leptin ameliorate Alzheimer’s disease-like tau protein hyperphosphorylation via Cdk5 pathway [D]. Master’s thesis of Chinese PLA General Hospital & Medicinal School of Chinese PLA, 2013 (in Chinese).

This Article


CN: 44-1286/R

Vol 42, No. 06, Pages 1380-1386

June 2019


Article Outline


  • 1 Materials and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References