Effect of Panaxatriol Saponin Enzymatic Hydrolysates from Panax ginseng Stem and Leaves on the Treatment of Alzheimer’s Mice Induced by Alchlor/D-Galactose

MA He-tong1 ZHU Hong-yan1 GAO Yu-gang1 HE Zhong-mei1,2 YANG He1 ZHAO Yan1 BI Yun-feng3

(1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China 130118)
(2.Key Laboratory of Effective Components Analysis of Changbai Mountain Chinese Medicinal Materials, Changchun, China 130118)
(3.College of Food Science, Jilin Agricultural University, Changchun, China 130118)

【Abstract】 Objective: To observe the therapeutical effect of panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves (GSLPSH) on the treatment of Alzheimer’s disease (AD). Methods: The AD model was established by administration of alchlor combined with D-galactose. The changes in learning and memory abilities of mice were measured by step-down test. The activities of acetylcholinesterase (AChE), glutathione peroxidase (GSH-Px), as well as phospho-Tau (P-Tau) and acetylcholine (ACH) content in mouse hippocampus, were detected by ELASA. The expressions of Tau protein, cyclin-dependent-like kinase 5 (CDK5), microtubule-associated protein 2 (MAP2), nuclear factor NF-kappa-B p65 (NF-κB p65), and amyloid beta (Aβ) proteins in mouse hippocampus were observed by immunohistochemistry. Results: GSLPSH could improve the learning and memory ability, reduce the levels of AChE and P-Tau, increase the levels of GSH-Px and ACH, inhibit the expressions of Tau, CDK5, NF-κB p65, and Aβ proteins, and promote the MAP2 protein expression in the hippocampus of AD mice induced by alchlor/D-galactose. Conclusion: GSLPSH has therapeutic effect on AD model mice induced by alchlor/D-galactose. It improves symptoms in AD mice by inhibiting the deposition of Aβ protein and the excessive phosphorylation of Tau protein, enhancing the activity of GSH-Px, and reducing the expression of inflammatory factor NF-κB p65.

【Keywords】 Panaxatriol saponin enzymatic hydrolysates from Panax ginseng stems and leaves; Alchlor/D-galactose; Alzheimer’s disease;

【DOI】

【Funds】 Soft Science Research Program of Jilin Province (20180414072GH)

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 42, No. 06, Pages 1380-1386

June 2019

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Article Outline

Abstract

  • 1 Materials and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References