Mechanism Research of Gambogenic Acid on Proliferation, Apoptosis and Invasion of Cervical Carcinoma HeLa Cells
【Abstract】Objective: To investigate the effect of gambogenic acid on proliferation, apoptosis and invasion of human cervical carcinoma HeLa cells. Methods: HeLa cells were given different concentration of gambogenic acid (0.00, 0.63, 1.25, 2.50, 5.00 and 10.00 mg/L) for 72 h, and MTT assay was used to measure cell proliferation. Flow cytometry was used to measure cell cycle. Fluorescence microscopy and DNA ladder analysis were used to measure the apoptosis of HeLa cells. Transwell chambers were adopted to detect the cells invasion. The expression levels of mRNA and protein of BCL-2, BAX, NF-κB and E-cadherin were detected by RT-PCR and Western blot. Results: After treatment with different concetrations of gambogenic acid for 72 h, the proliferation of HeLa cells was significantly inhibited in a concentration-dependent manner. The ability of invasion was decreased with the decrease of the concentration of gambogenic acid, which was detected by Transwell chamber assays in vitro. The RT-PCR and Western blot demonstrated that gambogenic acid up-regulated the expression of BAX and E-cadherin and down-regulated the expression of mRNA and protein of BCL-2 and NF-κB. Conclusion: Gambogenic acid can inhibit the proliferation and invasion of HeLa cells, and promote cell apoptosis in a concentration-dependent manner.
【Keywords】 Cervical carcinoma; HeLa cells; Gambogenic acid; Proliferation; Apoptosis; Invasion;
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