Effects of Total Ginsenosides and Volatile Oil of Acorus Tatarinowii Co-Administration on Ability of Learning and Memory and Apoptosis in Alzheimer's Disease Mice Model Induced By D-Galactose and Aluminium Chloride

DENG Min-zhen1 HUANG Li-ping1 FANG Yong-qi1

(1.The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China 510405)

【Abstract】Objective: To observe the effects of the co-administration of total ginsenosides and volatile oil of Acorus tatarinowii on the ability of learning and memory and apoptosis in Alzheimer's disease (AD) mice model induced by D-galactose and aluminium chloride. Methods: 50 Kunming (KM) mice were randomly divided into normal group, model group, Aricept group (1 mg/kg), Ding Zhi Wan group (10 g/kg) and co-administration of total ginsenosides and volatile oil of Acorus tatarinowii group (co-administration group, the doses of volatile oil of Acorus tatarinowii and total ginsenosides were 30 mg/kg and 150 mg/kg, respectively). In addition to normal group, mice in other groups were given D-galactose 150 mg /(kg·d), ip, and aluminium chloride 5 mg/kg, i.g., once daily for 40 days. At the same time, mice in the treatment groups were administrated with the corresponding drug from the 20th day after modeling, once daily for 40 days. Water maze and avoiding darkness experiments were conducted to test learning and memory abilities; Aβ1-42 and BCL-2 content in cortex and hippocampus were detected by ELISA; the vitalities of acetyl cholinesterase (AChE) and acetylcholine transferase (ChAT) were detected by ultraviolet spectrophotometry. Superoxide dismutase (SOD) vitalities were detected by a water-soluble tetrazolium salt (WST-1) method; the content of malondialdehyde (MDA) in cortex and hippocampus were detected by the thiobarbituric acid (TBA) method; senile plaque on Aβ1-42 precipitation were observed by immunohistochemistry; brain tissues were observed by hematoxylin-eosin staining (HE). Results: As compared with model group, in the co-administration group, the time of AD mice swimming, the numbers of blind area and electric shock reduced significantly (P < 0.05), and the latent period was prolonged (P < 0.05); AChE activity and levels of Aβ1-42 and MDA in cortex and hippocampus were decreased significantly (P < 0.05 or P < 0.01); ChAT and SOD activities as well as BCL-2 content were increased significantly (P < 0.05 or P < 0.01); the formation of senile plaque was decreased and brain tissue morphology was improved. Conclusion: Total ginsenosides and volatile oil of Acorus tatarinowii co-administration has an effect on improving the ability of learning and memory and inhibiting apoptosis.

【Keywords】 Alzheimer's disease; Volatile oil of Acorus tatarinowii; Total ginsenosides; Learning and memory; Apoptosis;

【DOI】

【Funds】 Science and Technology Project of Guangdong Province (2012A032400006)

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 1018-1023

May 2015

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Article Outline

Abstract

  • 1 Materials and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References