Effects of Total Ginsenosides and Volatile Oil of Acorus Tatarinowii Co-Administration on Ability of Learning and Memory and Apoptosis in Alzheimer's Disease Mice Model Induced By D-Galactose and Aluminium Chloride
【Abstract】Objective: To observe the effects of the co-administration of total ginsenosides and volatile oil of Acorus tatarinowii on the ability of learning and memory and apoptosis in Alzheimer's disease (AD) mice model induced by D-galactose and aluminium chloride. Methods: 50 Kunming (KM) mice were randomly divided into normal group, model group, Aricept group (1 mg/kg), Ding Zhi Wan group (10 g/kg) and co-administration of total ginsenosides and volatile oil of Acorus tatarinowii group (co-administration group, the doses of volatile oil of Acorus tatarinowii and total ginsenosides were 30 mg/kg and 150 mg/kg, respectively). In addition to normal group, mice in other groups were given D-galactose 150 mg /(kg·d), ip, and aluminium chloride 5 mg/kg, i.g., once daily for 40 days. At the same time, mice in the treatment groups were administrated with the corresponding drug from the 20th day after modeling, once daily for 40 days. Water maze and avoiding darkness experiments were conducted to test learning and memory abilities; Aβ1-42 and BCL-2 content in cortex and hippocampus were detected by ELISA; the vitalities of acetyl cholinesterase (AChE) and acetylcholine transferase (ChAT) were detected by ultraviolet spectrophotometry. Superoxide dismutase (SOD) vitalities were detected by a water-soluble tetrazolium salt (WST-1) method; the content of malondialdehyde (MDA) in cortex and hippocampus were detected by the thiobarbituric acid (TBA) method; senile plaque on Aβ1-42 precipitation were observed by immunohistochemistry; brain tissues were observed by hematoxylin-eosin staining (HE). Results: As compared with model group, in the co-administration group, the time of AD mice swimming, the numbers of blind area and electric shock reduced significantly (P < 0.05), and the latent period was prolonged (P < 0.05); AChE activity and levels of Aβ1-42 and MDA in cortex and hippocampus were decreased significantly (P < 0.05 or P < 0.01); ChAT and SOD activities as well as BCL-2 content were increased significantly (P < 0.05 or P < 0.01); the formation of senile plaque was decreased and brain tissue morphology was improved. Conclusion: Total ginsenosides and volatile oil of Acorus tatarinowii co-administration has an effect on improving the ability of learning and memory and inhibiting apoptosis.
【Keywords】 Alzheimer's disease; Volatile oil of Acorus tatarinowii; Total ginsenosides; Learning and memory; Apoptosis;
 Liao Yu, Dong Wen. Protective effect of the donepezil and verapamil co-administration on the learning and memory ability in AD mice model[J]. Sichuan Journal of Physiological Sciences, 2012, 34(2): 58–60. (In Chinese)
 He Ying, Wang Chao, Wang Decai. Research progress on the non-therapeutic drugs and new targets in Alzheimer's Disease treatment[J]. China Medical Herald, 2014, 11 (6): 162–166. (In Chinese)
 Wang Huping, Xing Xiping, Zhang Wenjuan, et al. Effects of Xiao Yao Powder on the learning and memory and transmitter in AD mice model[J]. Chinese Journal of Gerontology, 2014, 34(9): 2468–2470. (In Chinese)
 Yan Juanjuan, Liu Ming, Hu Yuan, et al. Effect and action mechanism of small Ding Zhi Wan on the learning and memory impairment of mice induced by scopolamine[J]. Chinese Materia Medica, 2012, 37(21): 3293–3296. (In Chinese)
 Zhang Danshen, Zhang Juntian. Effect of total ginsenosides on the memory impairment of mice induced by β-amyloid peptide[J]. Chinese Pharmacological Bulletin, 2000, 16(4): 422–425. (In Chinese)
 Kuang Zhongsheng, Jiang Yong, Li Zhiqiang, et al. Effect of Acorus tatarinowii extract on the NMDA and M receptor-ligand binding in cerebral cortex of mice[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2010, 21(4): 354–357. (In Chinese)
 Zheng Lu, Qiu Lei, Zhang Yao, et al. Effect of Radix Polygalae saponin on the improvement of learning and memory ability and the transmitter in rapidly-aging mice[J]. Journal of Beijing University of Traditional Chinese Medicine, 2010, 33(3): 183–186. (In Chinese)
 Zhang Min, Chen Dongxue, Sun Xiaomeng. Effect of Poria cocos aqueous extract solution on the learning and memory ability of mice[J]. Journal of Beihua University (Natural Science), 2012, 13 (1): 62–64. (In Chinese)
 Zhang Zhihua, Wang Xiulian, Liu Songlin, et al. Treatment of Alzheimer's Disease with the co-administration of Acorus tatarinowii and Radix Polygalae[J]. Hubei Journal of Traditional Chinese Medicine, 2012, 34(8): 32–34. (In Chinese)
 Li Furen, Chou Lili, Fan Xintian. Research on the Radix Polygalae Poria cocos alcohol extract improving the learning and memory impairment[J]. Journal of Beihua University (Natural Science), 2011, 12(2): 172–176. (In Chinese)
 Luo Huanmin, Xiao Fei. Study on D-galactose and aluminium trichloride inducing Alzheimer's Disease in mice model[J]. Hubei Journal of Traditional Chinese Medicine, 2004, 18(1): 22–26. (In Chinese)
Blennow K, de Leon MJ, Zetterberg H. Alzheimer's disease[J]. Lancet, 2006, 368:387–403.
Kotani S, Yamauchi T, Teramoto T, et al. Pharmacological evidence of cholinergic involvement in adult hippocampal neurogenesis in rats[J].Neuroscience, 2006, 142(2): 505–541.
 Ji Deli, Li Ziru, Guo Lijun. Apoptosis and Alzheimer's Disease[J]. Inner Mongolia Medical Journal, 2014, 46(3): 296–298. (In Chinese)
 Wang Li, Hu Kaming, Gao Huaiyun. Effect of electropuncture on the transmitter content in frontal lobe of senile Alzheimer's Disease rats[J]. Journal of Chengdu University of Traditional Chinese Medicine, 2012, 35(2): 25–30. (In Chinese)
Shang YZ, Gong MY, Zhou XX, et al. Improving effects of SSF on memory deficits and pathological changes of neural and immunological systems in senescent mice[J]. Acta Pharmacol Sin, 2001, 22(12):1078–1083.
 Wu Ziming, Peng Jianlong. Effect of Yizhiling granules on the experimental pathologic model of senile dementia[J]. Chinese Journal of Integrative Medicine, 1992, 12: 622–623. (In Chinese)