Effect of Acanthopanax senticosus Polysaccharides on Cardiac Endogenous Metabolism in Rats Based on Metabolomics

LU Fang1 YANG Xiao-dan1 JING Yue-e1 ZHANG Na1 LIU Shu-min2

(1.Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, China 150040)
(2.Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin, China 150040)

【Abstract】Objective: To study the influence of Acanthopanax senticosus polysaccharides on cardiac endogenous metabolism in rats using metabolomics method, in order to find potential biomarkers and analyze the metabolic pathways which can explore the pharmacological effects and mechanisms of action. Methods: 20 SD rats were randomly divided into two groups, the blank and Acanthopanax senticosus polysaccharides treatment groups, which were treated with saline and Acanthopanax senticosus polysaccharide for 20 days. On the 21st day, heart tissues were collected and each sample extract was analyzed by UPLC-Q-TOF/MS. Results: 20 potential biomarkers and 6 major metabolic pathways related to cardiovascular and cerebrovascular diseases were identified. Conclusion: Acanthopanax senticosus polysaccharides has a certain pharmacological effects on cardio-cerebro vascular diseases, cancer and other diseases. Its mechanism may be related to the metabolic process of amino acids, fatty acid and folate.

【Keywords】 Acanthopanax senticosus polysaccharides; Metabonomics; Marker; Cardio-cerebrovascular disease; Homocysteine;

【DOI】

【Funds】 National Natural Science Foundation of China for Youths (81270056) the Program for New Century Excellent Talents in University of Heilongjiang Province, China (1254-NCET-020) Research Fund of Heilongjiang University of Chinese Medicine (Outstanding Talents Training Fund) (2013jc01)

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 1004-1008

May 2015

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Article Outline

Abstract

  • 1 Materials and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • 5 Conclusion
  • References