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吴贵辉1,2 陈艳1 郑黎花1,2 黄滔2 郝小江2 张建新2

(1.贵州大学, 贵州贵阳 550025)
(2.贵州省中国科学院天然产物化学重点实验室, 贵州贵阳 550002)

【摘要】目的:对黔产杜仲进行化学成分研究。方法:利用硅胶、Sephadex LH-20、RP-18、MCI等柱色谱及半制备高效液相色谱等多种色谱技术进行分离纯化,根据化合物的波谱数据确定化合物结构。结果:从杜仲乙酸乙酯和正丁醇萃取部位中分离纯化了16个化合物,分别鉴定为:β-谷甾醇(1)、环桉树醇(2)、白桦脂酸(3)、24-亚甲基环阿屯酮(4)、环桉烯酮(5)、salicifoliol(6)、松脂素(7)、京尼平(8)、格链孢醇(9)、蛇菰脂醛素(10)、杜仲二醇(11)、松脂醇-4'-O-β-D-吡喃葡萄糖苷(12)、杜仲醇(13)、脱氧杜仲醇(14)、8-羟基松脂素(15)、去氢二松柏醇γ'-O-β-D-吡喃葡萄糖苷(16)。结论:其中,化合物2、4~6、9、10和15为首次从该植物中分离得到,化合物14为首次从杜仲皮中分离得到。

【关键词】 杜仲;化学成分;


【基金资助】 国家重大科学仪器设备开发专项(2011YQ12003506); 贵州省中药现代化科技产业研究开发专项(黔科合SY字【2014】3081-1号);

Chemical Constituents of Eucommia ulmoides in Guizhou Province

WU Gui-hui1,2 CHEN Yan1 ZHENG Li-hua1,2 HUANG Tao2 HAO Xiao-jiang2 ZHANG Jian-xin2

(1.Guizhou University, Guiyang, China 550025)
(2.The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang, China 550002)

【Abstract】Objective: To study the chemical constituents of Eucommia bark in Guizhou Province. Methods: Silica gel, Sephadex LH-20, RP-18, MCI and semi-preparative HPLC were used to study the chemical constituents of Eucommia bark, and the chemical structures were elucidated by application of spectral data. Results: 16 compounds were isolated from the bark of Eucommia ulmoides. Their structures were identified as β-sitosterol (1), cycloeucalenol (2), betulinic acid (3), 24-methylenecycloartenone (4), cycloeucalenone (5), salicifoliol (6), pinoresinol (7), genipin (8), alternariol (9), balanophonin (10), eucommidiol (11), pinoresinol-4'-O-β-D-glucopyranoside (12), eucommiol (13), deoxyeucommiol (14), 8-hydroxypinoresinol (15), and dehydrodiconiferyl alcohol γ’-O-β-D-glucopyranoside (16). Conclusion: Seven compounds, including compounds 2, 4–6, 9, 10 and 15 are isolated from Eucommia ulmoides Oliv. for the first time, and compound 14 is isolated from the bark of Eucommia ulmoides for the first time.

【Keywords】 Eucommia ulmoides Oliv.; Chemical constituents;


【Funds】 National Key Scientific Instrument and Equipment Development Project of China (2011YQ12003506); Science and Technology Industry Research and Development Fund for TCM Modernization of Guizhou Province ([2014] 3081-1);

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This Article


CN: 44-1286/R

Vol 38, No. 05, Pages 980-984

May 2015


Article Outline


  • 1 Instruments and materials
  • 2 Extraction and isolation
  • 3 Structural identification
  • References