Determination of Nucleosides and HPLC Fingerprints of Cordyceps

WANG Bing1 LI Ning2 DONG Ting-xia2 ZHAN Hua-qiang2

(1.Shanghai University of Traditional Chinese Medicine, Shanghai, China 201203)
(2.The Hong Kong University of Science and Technology, Hong Kong, China)
【Knowledge Link】stromata; degasser; centrifuge; buffer

【Abstract】Objective: To establish the HPLC fingerprints method of Cordyceps and to determine the content of uridine, inosine, guanosine and adenosine. Methods: The HPLC separation was performed on a Grace Prevail C18 column (150 mm × 4.6 mm, 5 μm) in a gradient elution mode with a mixture consisting of water and methanol at a flow rate of 1.0 mL/min. The detection wavelength was set at 260 nm and the column temperature was 25 °C. Results: The content of four nucleosides was determined in Cordyceps from different habitats, and the HPLC fingerprint of Cordyceps was set up with 13 common peaks. Among them, uridine, inosine, guanosine and adenosine were identified. The similarities of ten fingerprints were greater than 0.95 with good separation of each chromatographic peak, and met the requirement of the fingerprints. There were similar results in cluster analysis and principal component analysis of the major nucleosides and the fingerprints of 10 batches of Cordyceps. The results of sample classification in principal component analysis showed a good similarity with cluster analysis. Conclusion: This method showed the information of chemical composition in Cordyceps, with good repeatability and similarity between samples, indicating that the stable chemical distribution and proportion of the major nucleosides in the medical materials. Fingerprints, principal component analysis and cluster analysis, which are applied to identify the different sources of Cordyceps, provide an experimental basis for establishing the characteristics evaluation methodology of medicinal materials.

【Keywords】 Cordyceps sinensis (BerK.) Sacc.; Nucleosides; HPLC fingerprint; Cluster analysis; Principal component analysis;

【DOI】

Download this article

    References

    [1] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China[S]. Vol. I. China Medical Science Press, Beijing, 2010: 106 (in Chinese).

    [2] Paterson RR. Phytochmistry, 2008, 69(7): 1469–1495.

    [3] Xu JT. Chinese medicinal mycology[M]. Beijing: Peking University Health Science Center–Peking Union Medical College Associated Press, 1997: 754 (in Chinese).

    [4] Wu LF, Xing Y, Guan YL, et al. Chinese Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease, 2013, 11(10): 1254–1256 (in Chinese).

    [5] Li WF, Song J, Shi JY. Journal of Shandong University of Traditional Chinese Medicine, 2013, 37(4): 342–344 (in Chinese).

    [6] Aan FQ, Li DQ, Feng K, et al. J Chromatogr A, 2010, 1217(34): 5501–5510.

    [7] Song LY, Ye B, Yu RM, et al. Journal of Chinese Medicinal Materials, 2007, 30 (9): 1156-1157 (in Chinese).

This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 952-956

May 2015

Downloads:0

Share
Article Outline

Knowledge

Abstract

  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Discussion
  • References