HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma

WU Ying1 GAO Hui1 SONG Ze-bi1

(1.College of Pharmacy, Liaoning University of Traditional Chinese Medicine/Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine/Engineering Technology Research Center of Traditional Chinese Medicine Processing of Liaoning Province, Dalian, China 116600)

【Abstract】Objective: To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma. Methods: The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm × 4. 6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241.3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm. Results: The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined. Conclusion: The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.

【Keywords】 Salt rhizome of Anemarrhena asphodeloides Bge.; HPLC-PDA-CAD fingerprints; Assay;

【DOI】

【Funds】 National Natural Science Foundation of China (81102810) Doctoral Program of Higher Education of Ministry of Education of China (20112133120009)

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    References

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 942-947

May 2015

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Abstract

  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Methods and results of content determination
  • 4 Discussion
  • References