HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma

WU Ying1 GAO Hui1 SONG Ze-bi1

(1.College of Pharmacy, Liaoning University of Traditional Chinese Medicine/Key Laboratory of Processing Theory Analysis of State Administration of Traditional Chinese Medicine/Engineering Technology Research Center of Traditional Chinese Medicine Processing of Liaoning Province, Dalian, China 116600)

【Abstract】Objective: To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma. Methods: The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm × 4. 6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241.3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm. Results: The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined. Conclusion: The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.

【Keywords】 Salt rhizome of Anemarrhena asphodeloides Bge.; HPLC-PDA-CAD fingerprints; Assay;


【Funds】 National Natural Science Foundation of China (81102810) Doctoral Program of Higher Education of Ministry of Education of China (20112133120009)

Download this article


    [1] Chinese Pharmacopoeia Commission. Pharmacopeia of the People’s Republic of China[S]. Vol. I. Beijing: China Medical Science Press, 2010: 197-198. (In Chinese)

    [2] Ke Wei, Ma Chunhui, Zhang Rujuan, et al. Studies on HPLC-ELSD specific fingerprints of Rhizoma Anemarrhenae of different sources[J]. Journal of Pharmaceutical Analysis, 2008, 28(1): 100-103. (In Chinese)

    [3] Han Liping, Chen Xingyu, Xu Aijuan. Fingerprints spectrum of anemarrhenae rhizoma by HPLC-ELSD[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(1): 21-23. (In Chinese)

    [4] Luo Jie, Fan Xuhang, Cui Sijiao, et al. UPLC fingerprint of anemarrhenae rhizoma and its hierarchical cluster analysis[J].Chinese Journal of Mordern Applied Pharmacy, 2013, 30(1): 28-31. (In Chinese)

    [5] Huang Wei, Mao Chunqin, Song Kun, et al. Establishment of HPLC fingerprint of saline rhizoma anemarrhenae[J]. Journal of Shanghai University of Traditional Chinese Medicine, 2008, 22(3): 79-81. (In Chinese)

    [6] Li Chunmei, Gao Yonglin, Li Min, et al. Effects of saponins from anemarrhean asphodeloides Bge. on blood glucose level in mice [J]. Pharmacology and Clinics of Chinese Materia Medica, 2005, 21(4): 22-23. (In Chinese)

    [7] Liu Zhuo, Jin Ying, Liu Wanzhu, et al. Effects of SAaB on LPS-induced learning and memory disorders and inflammatory reaction in rat hippocampus[J]. Chinese Pharmacological Bulletin, 2010, 26(10): 1362-1366. (In Chinese)

    [8] Gao Hui. Study on the processing mechanism of saltine anemarrhenae rhizoma[D]. Shenyang: Liaoning University of Traditional Chinese Medicine, 2010: 84-88. (In Chinese)

    [9] Wu Ying, Gao Hui, Song Zebi. Determination of timosaponin BⅡand sarsasapogenin in Anemarrhenae Rhizoma before and after processing with salt-water by HPLC-CAD[J]. Drugs &Clinic, 2014, 29(7): 737-741. (In Chinese)

This Article


CN: 44-1286/R

Vol 38, No. 05, Pages 942-947

May 2015


Article Outline


  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Methods and results of content determination
  • 4 Discussion
  • References