Correlation analysis of the main biological characteristics of germplasm resources and gentiopicroside content in roots of Gentiana macrophylla

Zhou Wenping1 Zhao Hongfeng1 Wang Yafei 1 Zhang Huijuan1 Wang Zhezhi1 Cao Xiaoyan1

(1.National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China/Co-Innovation Center for Qinba regions' sustainable development, Shaanxi, China 710062)
【Knowledge Link】jaundice; inflorescence; germplasm

【Abstract】Objective: To analyze correlations between the main biological characteristics of germplasm resources and gentiopicroside content in roots of Gentiana macrophylla. Methods: The gentiopicroside content in roots from 40 portions of germplasm resources of Gentiana macrophylla was detected with the established high-performance liquid chromatography (HPLC), and then the correlations between the main biological characteristics of germplasm resources and the gentiopicroside content in roots of Gentiana macrophylla were analyzed with Spearman rank correlation in software SPSS 16.0 and the optimal scaling in the regression analysis. Results: the gentiopicroside content in roots from 40 portions of germplasm resources of Gentiana macrophylla was in the range of 7.56%–11.32%. It had greatly significant correlations with the shape of the rosette leaf and the density of the top inflorescence, e.g., it would rise with increase of the narrow degree of the rosette leaf and the density of the top inflorescence; it also had significant correlations with the colors of the calyxes and stems. The darker the color was, the higher the gentiopicroside content was. Conclusion: With improvement of the gentiopicroside content as the goal of excellent individual plants breeding, Gentiana macrophylla with the lanceolate rosette leaf, dense top inflorescence and red or reddish green calyxed and stems is the main breeding object.

【Keywords】 Gentiana macrophylla; Germplasm resources; Gentiopicroside; Correlation analysis;

【DOI】

【Funds】 Science and Technology Planning Project of Xi'an City (NC1206-5)

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 933-936

May 2015

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Abstract

  • 1 Materials and methods
  • 2 Results and analyses
  • 3 Discussion
  • References