Study on Stability of Human Intestinal Bacterial Biotransformation Model

YANG Xin1 CONG Bo1 WANG Ru-feng1 PENG Yu-shuai1 LIU Li-jia1 ZHAO Can1 YANG Xiu-wei2

(1.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102)
(2.School of Pharmaceutical Sciences,Peking University, Beijing 100191)

【Abstract】Objective: To investigate the stability of human intestinal bacterial biotransformation model using isoquercetin as the substrate of transformation. Methods: The in vitro transformation model was established using the intestinal bacteria from different volunteers, or different passages of the same volunteer in accordance with the Biotransformation Model of Human Intestinal Bacteria and Its Standard Operating Procedures to transform isoquercetin. Results: Within 24 hours, all models established with the intestinal bacteria from different volunteers could transform isoquercetin to quercetin, and the transformation efficiency was inclined to increase with the increase of the number of culture passages. Conclusion: The intestinal bacterial model established in accordance with the “standard operating procedures” is stable and the results obtained with this model are reproducible, which demonstrates the suitability of this model for the investigation of the chemical constituents of Chinese medicinal materials.

【Keywords】 Isoquercetin; Human intestinal bacteria; Biotransformation;

【DOI】

【Funds】 National Natural Science Foundation of China (81073018, 81274044) Research Project of Beijing University of Chinese Medicine (2014–JYBZZ–XS–102)

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This Article

ISSN:1001-4454

CN: 44-1286/R

Vol 38, No. 05, Pages 923-927

May 2015

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Article Outline

Abstract

  • 1 Materials and instruments
  • 2 Methods
  • 3 Results
  • 4 Discussion and conclusion
  • References