Study on Stability of Human Intestinal Bacterial Biotransformation Model
(2.School of Pharmaceutical Sciences,Peking University, Beijing 100191)
【Abstract】Objective: To investigate the stability of human intestinal bacterial biotransformation model using isoquercetin as the substrate of transformation. Methods: The in vitro transformation model was established using the intestinal bacteria from different volunteers, or different passages of the same volunteer in accordance with the Biotransformation Model of Human Intestinal Bacteria and Its Standard Operating Procedures to transform isoquercetin. Results: Within 24 hours, all models established with the intestinal bacteria from different volunteers could transform isoquercetin to quercetin, and the transformation efficiency was inclined to increase with the increase of the number of culture passages. Conclusion: The intestinal bacterial model established in accordance with the “standard operating procedures” is stable and the results obtained with this model are reproducible, which demonstrates the suitability of this model for the investigation of the chemical constituents of Chinese medicinal materials.
【Keywords】 Isoquercetin; Human intestinal bacteria; Biotransformation;
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