Effect of Crop Rotation and Biological Manure on Quality and Yield of “Chuju” Chrysanthemum morifolium and Continuous Cropping Soil Enzyme Activities

XIAO Xin1 ZHU Wei1 DU Chao1 SHI Ya-dong2 WANG Jian-fei1

(1.College of Urban Construction and Environmental Sciences, Anhui Science and Technology University, Fengyang, China 233100)
(2.Anhui Jutai Chuju Chrysanthemum morifolium Herbal Technology Co., Ltd., Chuzhou, China 239000)

【Abstract】Objective: To investigate the effects of chrysanthemum-wheat rotation system and biological manure on continuous cropping soil enzyme activities and quality and yield of Chrysanthemum morifolium. Methods: Field experiments were conducted at the research base of Anhui Jutai Chuju Chrysanthemum morifolium Herbal Technology Co., Ltd., in Shiji Town, Nanqiao Country, Anhui Province. Samples were collected from treatments under chrysanthemum-wheat rotation system receiving bio-organic manure application of 200 kg/667 m2, conventional chrysanthemum-wheat rotation system and chrysanthemum continuous cropping system. Results: Chrysanthemum-wheat rotation system and biological manure obviously influenced the quality and yield of Chrysanthemum morifolium and continuous cropping soil enzyme activities. Compared with chrysanthemum continuous cropping system, total flavonoids, chlorogenic acid, soluble sugar and free amino acid content, quantitative of ray floret, inflorescence diameter, diameter of tubular floret, number of branches, number of flower and yield of Chrysanthemum morifolium and the activities of urease, acid phosphatase, invertase and protease in soil increased to 42.59 mg/g, 2.52 mg/g, 4.04 mg/g, 73.33 mg/100 g, 179.56, 5.57 cm, 1.43 cm, 36.10, 330.00 and 400.09 kg/667 m2, respectively, while hydrogen peroxidase of soil under chrysanthemum-wheat rotation system decreased. Conclusion: Bio-organic manure application of 200 kg/667 m2 is beneficial to soil environment establishment of chrysanthemum-wheat rotation system and enhancement of quality and yield of Chrysanthemum morifolium while reducing the obstacles of continuous cropping.

【Keywords】 Biological manure; Rotation; “Chuju” Chrysanthemum morifolium Ramat.; Soil enzyme activities; Continuous cropping obstacle; Yield;


【Funds】 China Spark Program (2012GA710021) Key Natural Science Research Project for Colleges and Universities of Anhui Province (KJ2012A067) Key Scientific and Technological Project of Anhui Province (1301032151)

Download this article


    [1] Xie Yue, Xiao Xin, Zhou Yi, et al. Effects of five phenolic allelochemicals on growth and enzymes activity of Chuzhou chrysanthemum cutting seedling[J]. Journal of Nanjing Agricultural University, 2012, 35(6): 19-24 (in Chinese).

    [2] Wang Jianfei, Zhou Yi, Gao Xiang, et al. Isolation, identification and variation characteristics in soil of a continuous cropping Chuzhou chrysanthemum[J]. JOURNAL OF BIOLOGY, 2011, 28(6): 46-48 (in Chinese).

    [3] Lv Yi, Song Fuhai, Li Yuanyuan, et al. The influence of different crops rotation on the environment of soil and physiological characteristics of malus hupehensis Rehd. seeding[J]. Sciecntia Agricultura Sinica, 2014, 47(14): 2830-2839 (in Chinese).

    [4] Wu Hongliang, Kang Jianhong, Chen Fu, et al. Effect of different rotation patterns on soil microbial population and physiochemical properties under gravel-sand mulched field conditions[J]. Chinese Journal of Eco-Agriculture, 2013, 21(6): 674-680 (in Chinese).

    [5]Zewde T, Fininsa C, Sakhuja PK, et al.Association of white rot(Sclerotium cepivorum)of garlic with environmental factors and cultural practices in the North Shewa highlands of Ethiopia[J].Crop Protection, 2007, 26(10):1566-1573.

    [6] Li Xiaoliang, Cheng Tingting, Fang Hongxiang, et al. Effects of Water and Nitrogen Fertilizer Coupling on Yield and Quality of Chuzhou Chrysanthemum Morifolium[J]. Bulletin of Soil and Water Conservation, 2014, 34(2): 111-115 (in Chinese).

    [7] Wang Jianfei, Zhou Yi, Gao Xiang, et al. Effects of bio-organic fertilizer on the yield of chuzhou chrysanthemum and soil microflora under continuous cropping cultivation system[J]. Chinese Journal of Soil Science, 2014, 45 (2): 394-397 (in Chinese).

    [8] Xiao Xin, Li Yingfeng, Han Yitao, et al. Effects of urea fertilizer application on soil microbial activity of Chuzhou Chrysanthemum Morifolium Ramat. Journal of Soil and Water Conservation, 2011, 25 (5): 99-102 (in Chinese).

    [9] Li Peng, Chen Keli, Ye Congjin. Quality study of flos Chrysanthemi growing in Hubei Futianhe region[J]. Journal of Chinese Medicinal Materials, 2004, 27(2): 102-103 (in Chinese).

    [10] Chen Yonggan, Wang Li, Wang Huan. 2003, 17(3): 55-56. Determination of chlorogenic acid content in instant chrysanthemum tablets[J]. Research and Practice of Chinese Medicines, 2003, 17(3): 55-56 (in Chinese).

    [11] Li Hesheng. The experiment principle and technique on plant physiology and biochemistry[M]. Beijing: Higher Education Press, 2000: 192-197 (in Chinese).

    [12] Guan Songyin. Soil enzyme and its research method[M]. Beijing Agricultural Press, 1986: 274-323 (in Chinese).

    [13] Chinese Pharmacopoeia Commission. Pharmacopeia of the People’s Republic of China[S]. Vol. I. Beijing: China Medical Science Press, 2010: 292 (in Chinese).

    [14] Cai Yan, Hao Mingde. Effects of long-term rotation on the nutritional quality of wheat grain protein on dryland of Loess Plateau, Northwest China[J]. Chinese Journal of Applied Ecology, (5): 1354-1360 (in Chinese).

    [15]Sekamatte BM, Ogenga-Latigo M, Russell-Smith A.Effects of maize-legume intercrops on termite damage to maize, activity of predatory ants and maize yields in Uganda[J].Crop Protection, 2003, 22(1):87-93.

    [16] Xie Kai, Song Xiaohui, Dong Caixia, et al. Effects of different organic fertilizers on tree growth and soil property in Huangguan pear orchard[J]. Plant Nutrition and Fertilizer Science, 2013, 19(1): 214-222 (in Chinese).

    [17]Marten DA.Management and crop reside influence soil aggregate stability[J].Journal of Environmental Quality, 2000, 29(3):723-727.

    [18] Xu Peizhi, Xie Kaizhi, Chen Jiansheng, et al. effect of rice-vegetable cropping system with a mid-late rice on soil enzyme activity and cultivated microbial diversity[J]. Plant Nutrition and Fertilizer Science, 2008, 14(5): 923-928 (in Chinese).

    [19] Wu Huantao, Wei Min, Yang Fengjuan, et al. Improving effects of crop rotation and fallowing on continuous cucumber cropping soil in solar greenhouse[J]. Shandong Agricultural Science, 2008, (5): 59-63 (in Chinese).

    [20]Yang RY, Tang JJ, Chen X, et al.Effects of coexisting plant species on soil microbes and soil enzymes in metal lead contaminated soils[J].Applied Soil Ecology, 2007, 37(3):240-246.

This Article


CN: 44-1286/R

Vol 38, No. 05, Pages 889-893

May 2015


Article Outline


  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References