Effect of Panax japonicus saponin Ⅳa on alleviating nonalcoholic steatohepatitis by regulating miR-17-5p/MFN2 signaling pathway

LI Cong1 LIU Xiao-hui2 XIONG Hai-rong1 LI Xiao-xiao1 LUO Yue1 LIU Chao-qi1

(1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, Hubei, China 443002)
(2.Medical College of China Three Gorges University, Yichang, Hubei, China 443002)

【Abstract】To study the effect of Panax japonicus saponin Ⅳa (SPJ-Ⅳa) on nonalcoholic steatohepatitis (NASH) through miR-17-5p/MFN2 signaling pathway. The nonalcoholic steatohepatitis model was induced by a high-fat diet combined with CCl4 in Balb/c male mice. The mouse serum and liver were collected, the body weight and liver weight were measured. The liver index was calculated, and the serum biochemical indicators, i.e., alanine amino transferase (ALT), triglyceride (TG), and glucose (Glu), were measured. The morphological changes in the liver were detected by HE and Masson staining; real-time PCR was used to detect lipid metabolism-related genes, inflammation-related genes interleukin-6 (IL-6) and interleukin-1β (IL-1β), miR-17-5p and MFN2 expressions; Western blot was used to detect MFN2 protein expression level. Compared with the conditions in the normal group, the liver index in the HFD + CCl4 group was significantly increased, and the contents of ALT, TG, and Glu were significantly increased; the morphology showed obvious steatosis and collagen fiber deposition; mRNA expression levels of lipid metabolism-related genes, inflammation-related genes and miR-17-5p increased significantly; the mRNA expression level of MFN2 decreased significantly; the protein level of MFN2 decreased. After intervention with SPJ-Ⅳa, the levels of ALT, TG and Glu decreased; morphological steatosis decreased; collagen fiber deposition decreased; mRNA expression levels of lipid metabolism-related genes, inflammation-related genes and miR-17-5p decreased. The mRNA expression level of MFN2 increased, and the protein level of MFN2 also increased. The results of this study indicated that miR-17-5p/MFN2 signaling pathway may be involved in the occurrence and development of NASH, and SPJ-Ⅳa had a protective effect on NASH. Its mechanism may be related to the regulation of miR-17-5p/MFN2 signaling pathway.

【Keywords】 nonalcoholic steatohepatitis; CCl4; Panax japonicus saponin Ⅳa; MFN2; miR-17-5p;

【DOI】

【Funds】 National Natural Science Foundation of China (81673675) China Three Gorges University Dissertation Excellent Training Foundation Project (2019SSPY102)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 19, Pages 4725-4731

October 2020

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Article Outline

Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References