Quality evaluation of different Berberidis Cortex species based on 1H-NMR metabolomics and anti-diabetic activity
(2.School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China 611137)
【Abstract】To evaluate the quality differences of four mainstream species of Berberidis Cortex,1H-NMR metabolomics was applied to detect its primary and secondary metabolites, and the partial least squares discriminant analysis and analysis of variance were integrated to screen the differential metabolites between species. Furthermore, diabetic rat model was established by high fat diet and streptozotocin to assess differences in anti-diabetic activities among the four species. Sixteen compounds were simultaneously detected and identified, including alkaloids, organic acids, carbohydrates, and amino acids. Markers of interspecific difference were revealed as magnoflorine, jateorhizine, bufotenidine, and saccharose for the first time. Berberis vernae and B. kansuensis presented superior activities on reducing blood glucose level, improving insulin resistance, increasing insulin sensibility, and anti-inflammation. B. dictyophylla showed moderate anti-diabetic effect, while B. diaphana rendered inferior anti-diabetic capacity. Based on the contents of the four differential markers and the evaluation of anti-diabetic activity, the quality of the four Berberidis Cortex species was ranked as B. vernae ≈ B. kansuensis > B. dictyophylla > B. diaphana. These results provided references for species sorting, quality standard establishment, and exploitation of Berberidis Cortex. The anti-diabetic activities of B. vernae and B. kansuensis as well as their mechanisms of action merit further study in the future.
【Keywords】 Berberidis Cortex; quality evaluation; 1H-NMR metabolomics; type 2 diabetes; multiple species;
(Translated by WANG YX)
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