UPLC quantitative fingerprint research on Gardeniae Fructus based on chemical pattern recognition technology

XU Xin1 QI Hua-wen1 GAO De-song1 CAI Xin-na1 JIN Lei1,2 JIN Hong-li1,3 WANG Chao-ran1,3 LIU Yan-fang1,3 LIANG Xin-miao1,3

(1.DICP-CMC Innovation Institute of Medicine, Taizhou, Jiangsu Province, China 225300)
(2.Taizhou Institute of Science and Technology, Nanjing University of Science and Technology, Taizhou, Jiangsu Province, China 225300)
(3.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, China 116023)

【Abstract】Twenty-six batches of Gardeniae Fructus from different producing areas were collected for the development of the fingerprint, and the main components of Gardeniae Fructus were identified by liquid chromatography-mass spectrometry. The producing areas of Gardeniae Fructus were distinguished by chemical pattern recognition technology, and the index components of Gardeniae Fructus were quantified. A UPLC wavelength switching method was adopted, and the separation was carried out on a Waters Acquity UPLC HASS C18 (2.1 mm × 100 mm, 1.7 μm) column using the mobile phase of acetonitrile-0.5% formic acid in water for gradient elution. Principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were conducted for data extraction. The results showed that the similarity from 26 batches of Gardeniae Fructus was more than 0.89, and ten common peaks were defined. Sixteen compounds including monoterpenoids, iridoids, and diterpenoids were identified by reference substance identification, literature comparison and high-resolution mass spectrometry data analysis. The origin of Gardeniae Fructus was differentiated by PCA and OPLS-DA, and geniposide and crocin Ⅰ were screened as two differential quality markers. The contents of crocin Ⅰ, crocin Ⅱ and geniposide in Gardeniae Fructus from different places were different. These results will provide a reference for the geographical origin traceability of Gardeniae Fructus.

【Keywords】 Gardeniae Fructus; quantitative fingerprint; PCA; OPLS-DA; mass spectrometry; qualitative analysis;

【DOI】

【Funds】 Standardization and Internationalization Research Project on the Formulation of Quality Standards for Chinese Medicine (GZY-KJS-2018-016-6)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 18, Pages 4416-4422

September 2020

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Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References