Terpenoids from Toona sinensis

HU Jiang1 LI Jia-xun2 LI Qiang1 PENG Tian-feng1 SHI Xiao-dong1 LYU Tao1 CAI Jian1 JING Nian-hua1 YANG Wei-xing1 WANG Wei3

(1.Institute of Characteristic Medicinal Resource of Ethnic Minorities, Qujing Normal University, Qujing, Yunnan, China 655011)
(2.Tumor Hospital of Yunnan Province, the Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China 650118)
(3.Department of Pharmacy, Navy Medical Center of PLA, Navy Military Medical University, Shanghai, China 200052)

【Abstract】Eight terpenoids (1–8) were isolated from the ethyl acetate soluble fraction of 80% ethanol extract of leaf of Toona sinensis through various column chromatographies on silica gel, Sephadex LH-20, MCI and ODS. Their structures were elucidated as 8β-hydroxypimar-15-en-19-oic acid methyl ester (1), cedrodorol B (2), 11β-acetoxyobacunol (3), toonayunnanin D (4), toonaciliatone D (5), toonaciliatone A (6), cedrelone (7), and 11β-hydroxygedunin (8) based on their chemical and physicochemical methods and spectroscopic data. Compound 1 was a new pimaradiene diterpenoid and terpenoids 27 were isolated and identified from this plant for the first time. Compound 1 was tested in vitro for cytotoxic potential by employing MTT method and radical scavenging potential using DPPH test. As a result, compound 1 exhibited weak cytotoxic activity against three tested tumor cell lines (SMMC-7721, A549 and MCF-7) with IC50 values less than 40 μmol·L−1 and moderate radical scavenging activities with IC50 values of 74.3 μmol·L−1.

【Keywords】 Toona sinensis; Meliaceae; terpenoids; cytotoxicity; radical scavenging activities;

【DOI】

【Funds】 National Natural Science Foundation of China (31760341) 2016 and 2017 Innovation and Entrepreneurship Training Project of Yunnan Undergraduates

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 18, Pages 4411-4415

September 2020

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Article Outline

Abstract

  • 1 Materials
  • 2 Extraction and separation
  • 3 Structure identification
  • 4 Anti-tumor activity in vitro
  • 5 Radical scavenging activity by DPPH test
  • 6 Discussion
  • References