Effect and mechanism of Bidens pilosa decoction on non-alcoholic fatty liver disease induced by high fat and high glucose in mice

GAO Xiao-le1 DUAN Leng-xin1 QIU Ke-ke1 GUO Man-lin1 JIAO Ye-lin1 WANG Dong-mei1

(1.Medical School, Henan University of Science and Technology, Luoyang, Henan, China 471023)

【Abstract】This study aimed to investigate the effect and possible mechanism of Bidens pilosa decoction on non-alcoholic fatty liver disease (NAFLD) induced by high-fat and high-glucose diet in mice. Balb/c mice were randomly divided into normal group, model group, metformin (200 mg·kg−1) treatment group, B. pilosa decoction (10 g·kg−1) treatment group, and combination treatment of metformin and B. pilosa decoction (100 mg·kg−1 + 5 g·kg−1) group. Except for the normal group, mice in other four groups were fed with high-fat and high-glucose diet for eight weeks to establish NAFLD model. After four weeks of treatment, blood was collected from the eyeballs, and the mice were sacrificed. Then the relevant indicators were detected. The results showed that compared with the model group, blood lipid and blood glucose levels of each treatment group were significantly decreased (P < 0.05); HE staining results showed that liver pathological damage in each treatment group was significantly improved; oil red O staining results showed that fat distribution in each treatment group significantly reduced (P < 0.01); immunohistochemical staining showed that the expression of glucose-regulated protein78 (GRP78) in liver tissues of each treatment group was also significantly reduced (P < 0.01); Western blot results showed that endoplasmic reticulum (ER) stress signaling pathway-related factors GRP78, phosphorylated-protein kinase R-like ER kinase (p-PERK), eukaryotic translation-initiation factor 2α (eIF2α), activating transcription factor 4 (ATF4), C/EBP homologous protein (Chop), inositol requiring 1α (IRE1α), and cleaved-cysteinyl aspartate specific proteinase 12 (cleaved-caspase-12) were significantly reduced (P < 0.01). The results of the combination treatment group were better than those of the single drug treatment group. These results showed that B. pilosa decoction had the effect in improving non-alcoholic fatty liver, and its mechanism may be related to the down-regulation of the expression of ER stress-related factors, and the reduction of the apoptosis of hepatocytes caused by ERS and the down-regulation of blood lipid and blood glucose levels.

【Keywords】 Bidens pilosa decoction; metformin; high fat and high glucose; non-alcoholic fatty liver disease (NAFLD); endoplasmic reticulum stress (ERS);

【DOI】

【Funds】 National Natural Science Foundation of China (81601225)

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(Translated by XING Y)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 16, Pages 3915-3921

August 2020

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References