Simultaneous determination and analysis of amino acids and nucleosides in Dendrobium by UHPLC-QTRAP-MS/MS

LIU Jun-lin1,2 YU Nian-jun1,2,3,4 XING Li-hua1,2 WANG Rui1 XU Jun1,2 PENG Dai-yin1,2,3,4 TONG Xiao-hui1 OU Jin-mei1 HE Xiang-lin5

(1.Anhui University of Chinese Medicine, Hefei, Anhui Province, China 230012)
(2.Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei, Anhui Province, China 230012)
(3.Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, Anhui Province, China 230012)
(4.Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, Anhui Province, China 230012)
(5.Huoshan Changchong Chinese Medicinal Material Development Co., Ltd., Huoshan, Anhui Province, China 237200)
【Knowledge Link】saccharomyces cerevisiae; nucleoside

【Abstract】By using multivariate statistical analysis to evaluate the essential quality and provide scientific basis for comprehensive utilization, we established an UHPLC-QTRAP-MS/MS method for the fast, precise, and efficient determination of 21 amino acids and 10 nucleosides in different species of Dendrobium. The analysis was performed on a Waters XBridge Amide column (2.1 mm × 100 mm, 3.5 μm) with gradient elution by mobile phase of 0.2% formic acid in water-0.2% formic acid in acetonitrile at a flow rate of 0.2 mL·min−1 with the column temperature at 30 °C. The target compounds were analyzed by the positive-ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of different species of Dendrobium was carried out by PCA and TOPSIS. All the 21 amino acids and 10 nucleosides showed good linearity among certain concentration range (r > 0.999), and the RSD values of the stability, precision, and repeatability were less than 3.0%. The recovery rate was in the range from 93.31% to 107.5%, and the RSD value was in the range of 1.1%–3.7%. The comprehensive evaluation index obtained with PCA indicated that D. huoshanense was significantly higher than others regarding amino acids and D. officinale was higher than others regarding nucleosides. The biggest Ci difference of TOPSIS was 68.7%, and the comprehensive evaluation demonstrated that D. huoshanense had the highest comprehensive quality. The method is precise, fast, and efficient and can provide a reliable basis for further research and intrinsic quality control of Dendrobium.

【Keywords】 Dendrobium; amino acids; nucleoside; content determination; UHPLC-QTRAP-MS/MS;


【Funds】 Joint Fund Project of National Natural Science Foundation (U19A2009) Anhui University Collaborative Innovation Project (GXXT-2019-043) Synergetic Innovation Center Project of Anhui Authentic Chinese Medicine Quality Improvement ([2013] No. 2) Project by Department of Education of Anhui Province ([2014] No. 44) Natural Key Project of Anhui University of Chinese Medicine (2019zrzd02) Forestry Standardization Demonstration Zone Construction Project for D. huoshanense Seedling Propagation and Cultivation Technology (Z175070050002)

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This Article



Vol 45, No. 16, Pages 3890-3899

August 2020


Article Outline



  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References