Proteomics and peptidomics analysis of Sepiae Endoconcha

LIU Rui1,2,3 WEI Shuang1,2,3 WANG Xin-zhi1,2,3 CHENG Jian-ming1,2,3 WU Hao1,2,3

(1.Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing, Jiangsu, China 210023)
(2.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China 210023)
(3.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China 210023)

【Abstract】Shotgun-based proteomics and peptidomics analyses were used to investigate the proteins and peptides in marine Chinese medicinal Sepiae Endoconcha (cuttlebone). Peptides were extracted from Sepiae Endoconcha by acidified methanol, and then strong cation exchange (SCX) resin was used to enrich those peptides. Also, proteins from Sepiae Endoconcha were extracted and digested by trypsin. Nano-LC Q Exactive Orbitrap mass spectrometry was used to analyze proteins and peptides from Sepiae Endoconcha. As a result, a total of 16 proteins and 168 peptides were identified by protein database search, and 328 peptides were identified by De novo sequencing. The identified proteins were hemocyanin, enolase, myosin, actin, calmodulin, etc., and the identified peptides were derived from actin, histone, and tubulin. All these proteins and peptides were important components in Sepiae Endoconcha, which would provide important theoretical and research basis for marine Chinese medicinal Sepiae Endoconcha investigations.

【Keywords】 marine Chinese medicinal; Sepiae Endoconcha (cuttlebone); protein; peptide; material basis;

【DOI】

【Funds】 Excellent Young Teachers (Qinglan) Project of Universities in Jiangsu Province (2016) The Third Level of “333 Project” in Jiangsu Province (2016) Special Project on Marine Science and Technology Innovation in Jiangsu Province (HY2017-7) Collaborative Innovation Project of Industry Chain of Standardized Breeding and Comprehensive Utilization of Medicinal South Pearl (Bhsfs002) Major Natural Science Research Project of Higher Education Colleges in Jiangsu (17KJA360005)

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(Translated by ZHAO B)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 16, Pages 3883-3889

August 2020

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References