Microscopic histochemical comparison during mountain-agarwood formation

GAO Jia-qi1,2 LIU Juan2 JIAO Li-chao3 YIN Ya-fang3 CHAI Xing-yun4 CHEN Su-yi-le5 HUANG Lu-qi1,2

(1.School of Pharmacy, Jiangsu University, Zhenjiang, China 212013)
(2.National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
(3.Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing, China 100091)
(4.Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China 100029)
(5.Alashan League Mongolian Medicine Hospital, Alxa, China 750300)

【Abstract】Mountain-agarwood plays an important role in ethnic medicine in China for its pharmaceutical value. Modern pharmacological researches demonstrated that mountain-agarwood was effective for its anti-myocardial ischemia, antibacterial, anti-inflammatory, antitumor and analgesic effects. Mountain-agarwood derives from the peeled roots, stems or twigs of Syringa pinnatifolia which belongs to Syringa genus. It often depends on the purple substance and fragrance to estimate the formation of mountain-agarwood. However, the mechanism of mountain-agarwood formation has not been reported. To observe the microcosmic change in the process during the formation of mountain-agarwood, this study described the microscopic and histochemical characteristics of mountain-agarwood formation through histochemical staining. Our results showed that a significant difference of the distribution of tyloses existed during mountain-agarwood formation. It was observed that inchoate mountain-agarwood had more starch granules and viable cells than mountain-agarwood formed with high level or low level. The amount of polysaccharide and degree of lignification were increased during the mountain-agarwood formation. The results indicated that the mountain-agarwood, which meets the quality requirements for pharmaceutical use, contained the following characteristics: a large amount of purple tyloses in heartwood; yellow-brown tyloses distributing in heartwood and sapwood which were less in the latter; lignification with high level; a few viable cells; lots of polysaccharide and few starch granules in xylem rays cell. This study is aimed to reveal the change of histochemical characteristics during mountain-agarwood formation, and lay the foundation for exploring the mechanism of mountain-agarwood formation.

【Keywords】 Syringa pinnatifolia; mountain-agarwood formation; histochemistry; microscopic observation;

【DOI】

【Funds】 National Key Research and Development Program Project (2019YFC1711000) Major Expenditure Increase/Reduction Project at the Central Level (2060302) Fundamental Research Funds for Central Public Welfare Scientific Research Institutes (ZZ13-YQ-093, ZZXT201904)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 16, Pages 3797-3804

August 2020

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References