Investigation of modulating effect of Qingfei Paidu Decoction on host metabolism and gut microbiome in rats
(2.Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University, Huzhou, Zhejiang, China 313000)
(3.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, China 116023)
(4.Innovation Institute of Medicine, China Medical City, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Taizhou, Jiangsu, China 225316)
(5.Department of Integrated Traditional Chinese and Western Medicine, Peking University Health Science Center, Beijing, China 100191)
(6.Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China 100039)
(7.School of Pharmacy, Second Military Medical University, Shanghai, China 200433)
【Abstract】This study is to explore the effect of Qingfei Paidu Decoction (QPD) on the host metabolism and gut microbiome of rats with metabolomics and 16S rDNA sequencing. Based on 16S rDNA sequencing of gut microbiome and metabolomics (GC-MS and LC-MS/MS), we systematically studied the serum metabolite profiles and gut microbiota composition of the rats treated with QPD for continued 5 days by oral gavage. A total of 23 and 43 differential metabolites were identified based on QPD with GC-MS and LC-MS/MS, respectively. The involved metabolic pathways of these differential metabolites included glycerophospholipid metabolism, linoleic acid metabolism, TCA cycle and pyruvate metabolism. Meanwhile, we found that QPD significantly regulated the composition of gut microbiota in rats, such as enriched Romboutsia, Turicibacter, and Clostridium_sensu_stricto_1, and decreased norank_f_Lachnospiraceae. Our current study indicated that short-term intervention of QPD could significantly regulate the host metabolism and gut microbiota composition of rats dose-dependently, suggesting that the clinical efficacy of QPD may be related with the regulation on host metabolism and gut microbiome.
【Keywords】 Qingfei Paidu Decoction; COVID-19; metabolomics; gut microbiome; inflammation; immune;
(Translated by ZHAO B)
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