Genome-wide identification and expression analysis of CSLA gene family of Dendrobium catenatum

GAO Ya-qian1 CHEN Xue-liang1 CHEN Dong-hong1 LIU Jing-jing1 SI Jin-ping1

(1.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, Zhejiang, China 311300)

【Abstract】Glucomannan is the key active ingredient of Dendrobium catenatum, and CSLA family is responsible for glucomannan biosynthesis. In order to systematically evaluate the CSLA family members of D. catenatum, the bioinformatics methods were performed for genome-wide identification of DcCSLA gene family members through the genomic data of D. catenatum downloaded from the NCBI database, and their phylogenetic relationship, gene structure, protein conserved domains and motifs, promoter cis-elements and gene expression profiles in response to stresses were further analyzed. The results showed that D. catenatum contained 13 CSLA members, all of which contained 9–10 exons. In the evolutionary relationship, CSLA genes were clustered into 5 groups, and DcCSLA genes were distributed in all branches. The ancestral genes of group Ⅰ existed before the monocot-dicot divergence, and groups Ⅱ–Ⅴ only existed in the monocot plants, indicating that group Ⅰ represents the earliest origin group. CSLA proteins were the characteristic of the signature CESA_CaSu_A2 domain. Their promoter regions contained cis-elements related to stresses and hormones. Under different stress treatments, low temperature induced the expression of DcCSLA5 and inhibited the expression of DcCSLA3. Infection of Sclerotium delphinii inhibited DcCSLA3/4/6/8/9/10 expression. Under the treatment of jasmonic acid, the DcCSLA11 expression was significantly up-regulated, and DcCSLA2/5/7/12/13 were significantly down-regulated. These results laid a foundation for further study on the function of DcCSLA genes in glucomannan biosynthesis and accumulation.

【Keywords】 Dendrobium catenatum; glucomannan; CSLA; gene expression; stress treatment;

【DOI】

【Funds】 National Key R&D Program of China (2017YFC1702201) National Natural Science Foundation of China (81603228) Xinmiao Talent Project of Zhejiang Province (2019R412041)

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(Translated by ZHAO B)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 13, Pages 3120-3127

July 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Conclusions and discussion
  • References