Simultaneous determination of four components of Shuganning Injection in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study
(2.Guizhou Ruihe Pharmaceutical Co., Ltd., Guiyang, Guizhou, China 550000)
【Abstract】This study aims to reveal the pharmacokinetics of Shuganning Injection in normal rats. In this experiment, ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry (UPLC-ESI-MS/MS) was used to establish an analytical method for simultaneous determination of chlorogenic acid, gardenioside, oroxylin A and baicalin in rat plasma. Then, the non-compartmental model (NCA) in Phoenix WinNonLin 6.4 software was used to fit pharmacokinetic parameters. The methodological validation showed that the linear relationship of the components in rat plasma samples was good (r > 0.995). The recovery rate and matrix effect of plasma samples with low, middle and high concentration were 79.14%–101.4%. The intra-day and inter-day precision, accuracy and stability met the requirements of biological sample analysis. The half-life (t1/2) of chlorogenic acid, gardenioside, oroxylin A did not change significantly and the area under blood concentration-time curve (AUC0–t) was proportional to the dose, which suggested that three components showed a linear kinetic characteristics, but baicalin showed nonlinear kinetic characteristics. Moreover, the retention time of each component in rats was short. The established UPLC-MS/MS quantitative analysis method was rapid, sensitive and accurate, which can be used for the determination of chlorogenic acid, gardenioside, oroxylin A and baicalin in rat plasma and pharmacokinetic study of Shuganning Injection.
【Keywords】 Shuganning Injection; chlorogenic acid; gardenioside; oroxylin A; baicalin; pharmacokinetics;
(Translated by FU LJ)
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