Inhibitory effect of paeonol on aortic endothelial inflammation in atherosclerotic rats by up-regulation of caveolin-1 expression and suppression of NF-κB pathway

LIU Ya-rong1,2 SHAO Qian1 ZHANG Hui-hui1 JIA Ying1 DAI Min1,2

(1.Department of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei, Anhui, China 230012)
(2.Key Laboratory of Xin’an Medicine, Hefei, Anhui, China 230012)

【Abstract】To explore whether paeonol can play an anti-atherosclerotic role by regulating the expression of aortic caveolin-1 and affecting NF-κB pathway, so as to inhibit the inflammatory response of vascular endothelium in atherosclerotic rats. The atherosclerotic model of rats was induced by high-fat diet and vitamin D2. The primary culture of vascular endothelial cells (VECs) was carried out by tissue block pre-digestion and adherent method. The injury model of VECs was induced by lipopolysaccharide (LPS), and filipin, a small caveolin inhibitor, was added for control. HE staining was used to observe pathological changes of aorta. TNF-α, IL-6 and VCAM-1 were detected by ELISA. Western blot assay was used to detect the protein expression levels of caveolin-1 and p65 in aorta and VECs. The results showed that as compared with model group, paeonol significantly reduced aortic plaque area and lesion degree in rats, decreased the level of serum TNF-α, IL-6 and VCAM-1 in the rats and enhanced the relative expression level of caveolin-1, decreased p65 expression conversely (P < 0.05 or P < 0.01). In vitro, as compared with model group, paeonol obviously improved cell morphology, decreased the secretion of TNF-α, IL-6 and VCAM-1 in VECs, increased caveolin-1 expression, and decreased p65 protein expression (P < 0.05 or P < 0.01). Furthermore, filipin could reverse the effect of paeonol on expression of inflammatory factors and proteins (P < 0.05 or P < 0.01). According to the results, it was found that paeonol could play the role of anti-atherosclerosis by up-regulating the expression of caveolin-1 and inhibiting the activation of NF-κB pathway to reduce vascular inflammation in atherosclerotic rats.

【Keywords】 caveolin-1; paeonol; atherosclerosis; aorta; vascular endothelial cell; inflammation;

【DOI】

【Funds】 National Natural Science Foundation of China (81773937) Natural Science Research Project of Anhui University of Traditional Chinese Medicine (2018zrd06)

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This Article

ISSN:1001-5302

CN:11-2272/R

Vol 45, No. 11, Pages 2578-2585

June 2020

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References