Expression analysis of transcription factor ERF gene family of Panax ginseng
(2.National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
【Abstract】Ethylene responsive factor (ERF), one of the largest families of transcription factors in plants, plays a key role in secondary metabolism of herbal plants. To analyze the expression of ERF family genes, this study used the heat map clustering method to analyze the ginseng transcriptomes of different parts and different growth years. The content of ginsenosides Rg1, Re and Rb1 in various concentrations of MeJA-treated ginseng adventitious roots was determined by the UPLC-MS/MS method. The expression of key genes of ginsenoside biosynthesis (DDS, CYP716A47, CYP716A53v2) and ERF family genes in MeJA-treated P. ginseng adventitious roots was determined by using real-time quantitative PCR. Pearson correlation was adopted to analyze the gene expression patterns of DDS, CYP716A47, CYP716A53v2 gene and ERF family. The results showed that the content of protopanaxadiol ginsenoside Rb1 in P. ginseng adventitious roots treated with different concentrations of MeJA increased, and the content of protopanaxatriol ginsenoside Rg1 and Re decreased. It is consistent with the increase in DDS and CYP716A47 expression and the decrease in CYP716A53v2 gene expression. The expression of ERF003, ERF118 and ERF012 genes was significantly positively correlated with CYP716A53v2, but negatively correlated with DDS. While the expression of ERF1B was significantly negatively correlated with CYP716A47. It is proved that ERF003, ERF118 and ERF012 were likely to inhibit the expression of DDS and promote the expression of CYP716A53v2, and ERF1B was likely to inhibit CYP716A47. This work could provide a theoretical basis of ERF functional verification of regulating the biosynthesis of ginsenosides.
【Keywords】 Panax ginseng; ginsenoside biosynthesis; transcription factor; ERF;
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