Effects of constant temperature on growth and gonad development of overwintering Whitmania pigra

ZHANG Hui-ning1 GUO Qiao-sheng1 SHI Hong-zhuan1 CHEN Pan-pan1 XUAN Han-mei1 YANG Pei-fang1 GUO Li-yuan1

(1.Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing, China 210095)

【Abstract】The present study was conducted to explore the effect of constant temperature overwintering on the growth, gonadal development and internal quality of the Wh. pigra which were in overwintering period. Wh. pigra which in overwintering period were placed in light incubator at 21 ℃, and the Wh. pigra that overwintered under natural conditions were used as control. That the changes of growth performance, gonad index, internal quality of two groups of Wh. pigra were measured at the end of the overwintering. Simultaneously the tissue slice technique was used to observe the morphological structure of the spermary and ovary of the two groups. The results showed that the body weight of constant temperature overwintering Wh. pigra which were placed in light incubator at 21 ℃ was 2.35 times that of natural overwintering Wh. pigra which overwintered under natural conditions, and the weight of female gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 11.54% higher than that of Wh. pigra which overwintered under natural conditions, and the weight of male gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 48.33% higher than that of the Wh. pigra which overwintered under natural conditions. At the same time, that vitellogenesis cells and film forming cells which in ovarian vesicles were significantly higher than those of the Wh. pigra which overwintered under natural conditions, and that spermatogonia and primary spermatocytes which in seminal vesicle were significantly higher than those of Wh. pigra which overwintered under natural conditions. Most important of all, the anti-thrombin activity of Wh. pigra which were placed in light incubator at 21 ℃ increased by 27.85% compared with the Wh. pigra that overwintered under natural conditions. In conclusion, that constant temperature can promote the growth, the development of sperm and egg cells, and the increase of anti-thrombin activity of Wh. pigra which were in overwintering period.

【Keywords】 Whitmania pigra; hibernation; growth; gonadal development;

【DOI】

【Funds】 National Natural Science Foundation of China (Grant No. 81673538, 81473306) Jiangsu Modern Agricultural Industrial Technology System Construction Project (Grant No. JATS [2019] 426, JATS [2019] 276)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 08, Pages 1873-1878

April 2020

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Article Outline

Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results and analysis
  • 4 Discussion
  • References