Effects of nutrients deficiency on growth and saponin accumulation of Panax quinquefolium

LI Jun-fei1 SHAO Hui-hui1 BI Yan-meng1 JIAO Xiao-lin1 ZHANG Xi-mei1 GAO Wei-wei1

(1.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China 100193)

【Abstract】Mineral nutrient elements are the key factors to maintain the growth and quality of Panax quinquefolium. In order to understand the comprehensive effect of different nutrient elements deficiency on P. quinquefolium, 2-year-old P. quinquefolium samples were cultivated by Hoagland complete nutrient solution (CK) or 10 different nutrients deficiency solution in sand culture. During the cultivation, the deficient symptom was observed. The plant height, leaf area, biomass, photosynthetic indicator, root activity, ginsenoside content were measured. The results showed that N, K or Fe deficiency could lead to leaves of P. quinquefolium yellowing. N, K, Ca, Mg and B deficiencies were the main factors decreasing plant height and leaf area. The biomass of plant decreased significantly in all the nutrient deficiency treatments (P < 0.05) compared with that in the control group, and that in the N, K, Ca or Fe deficiency groups descended over 50%. In the absence of N, K and Fe elements, the Pn, Gs, Ci, Tr and chlorophyll of leaves were decreased mostly. The first three factors decreasing root activity were N, K and Ca deficiencies. The effects of nutrient deficiency on saponins of P. quinquefolium were different. Generally, N, P, B, Zn and Cu deficiencies resulted the synthesis of saponins decreased significantly (P < 0.05). This study contributed to clarify the demand characteristics of P. quinquefolium for different nutrient elements, which is of great significance for the diagnose of nutrient deficiency, the rational fertilizer and the improvement of yield and quality of P. quinquefolium.

【Keywords】 P. quinquefolium; nutrient deficiency; symptom; growth; physiological characteristic; saponin;

【DOI】

【Funds】 Medicine and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences (2016-I2M-1-012) National Special Project for Basic Science and Technology Research of China (2015FY111500-044) Key R&D project of Shandong Province (Major Science and Technology Innovation Project) (2019JZZY020905)

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(Translated by ZHAO B)

    References

    [1] LI J, CHAI J B, ZHAO W G. Empirical study of American ginseng on anti-defatigation action and the effects of delayed hypersensitivity and the function of mononuclear phagocyte [J]. Chinese Archives of Traditional Chinese Medicine, 2007, 25 (10): 2002 (in Chinese).

    [2] LI Y, MA X L, QU S C, et al. Effects of CPPQ on immunologic function of immunosuppressive mice induced with cyclophosphamide [J]. Journal Of Norman Bethune University Of Medical Science, 1996, 22 (2): 137 (in Chinese).

    [3] ZHANG L X, PENG J M, Ma J. Study progress on nutrient deficiency of plants [J]. Chinese Agricultural Science Bulletin, 2010, 26 (8): 157 (in Chinese).

    [4] FU L Z, ZHAO L M, LYU H Q, et al. Effects of nitrogen level on growth of Tetrastigma hemsleyanum and phytochemical content and antioxidant activity in stems and leaves [J]. China Journal of Chinese Materia Medica, 2019, 44 (4): 696 (in Chinese).

    [5] CHEN Z, ZHAO Y J, MA X J, et al. Studies on the nutritional requirements of American ginseng (Panax quinquefolium) Ⅱ. effects of elementary N, P and K on it growth [J]. Chinese Traditional and Herbal Drugs, 1990, 21 (5): 29 (in Chinese).

    [6] WANG T S, REN Z X, MENG F Y. Study on the absorption of ten nutrient elements by Panax quinquefolium [J]. Ginseng Research, 1990 (1): 22 (in Chinese).

    [7] SUN L M, XING X, LI B et al. Experimental study on the requirement of nitrogen, phosphorus and potassium in Panax quinquefolium of different growth years in Wendeng [J]. Special Economic Animal and Plant, 2015, 196 (10): 35 (in Chinese).

    [8] LI H S. Principles and techniques of plant physiological and biochemical experiments [M]. Beijing: Higher Education Press, 1999 (in Chinese).

    [9] JIAO X L, LU X H, CHEN J A, et al. Effects of Fusarium solani and F.oxysporum infection on the metabolism of ginsenosides in American ginseng roots [J]. Molecules, 2015, 20 (6): 10535.

    [10] FENG G Q, JIN H, CHEN Z J, et al. Effect of different nutrient elements on the growth of Panax notoginseng [J]. Research and Practice on Chinese Medicines, 2003, 17 (S1): 18 (in Chinese).

    [11] ZHANG Y F, GUO B L, YANG X B, et al. Effects of nitrogen on yields and flavonoids contents of Epimedium pseudowushanense in hydroponic conditions [J]. China Journal of Chinese Materia Medica, 2017, 42 (23): 4574 (in Chinese).

    [12] WU W H. Plant physiology [M]. Beijing: Science Press, 2008 (in Chinese).

    [13] GUO L L, GUO S, DONG L L, et al. Key techniques for precision cultivation of nitrogenous fertilizer of pollution-free ginseng [J]. China Journal of Chinese Materia Medica, 2018, 43 (7): 1427 (in Chinese).

    [14] XU C L, SUN H, WANG Q X, et al. Effects of low potassium stress on some physiological characters of Panax quinquefolium[J]. Journal of Jilin Agricultural University, 2016, 38 (1): 63 (in Chinese).

    [15] YANG Z. Study on the effect of calcium to Ginseng [D]. Beijing: Chinese Academy of Agricultural Sciences, 2016 (in Chinese).

    [16] XIE C X, SUO F M, JIA G L, et al. Correlation between ecological factors and ginsenosides [J]. Acta Ecologica Sinica, 2011, 31 (24): 7551 (in Chinese).

    [17] Meng X J, Zhang P, Liu T. Absorption of nitrogen by ginseng and effect of nitrogen on 14 c assimilate distribution [J]. Acta Agriculturae Nucleatae Sinica, 1999, 15 (1): 35 (in Chinese).

    [18] SUO B H, ZHANG P, GUO S W, et al. Relation of between phosphate fertilizer and phosphorus nutrition, carbohydrate, ginsenosides of ginseng root [J]. Journal Of Jilin Agricultural University, 1994, 16 (4): 87 (in Chinese).

    [19] MING Q L, HAN T, HUANG F, et al. Advances in studies on ginsenoside biosynthesis and its related enzymes [J]. Chinese Traditional and Herbal Drugs, 2010, 41 (11): 1913 (in Chinese).

    [20] ZHOU W, WU X Y, ZHANG L, et al. Gene cloning, prokaryotic expression and bioinformatics analysis of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) from Gentiana rigescens [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24 (22): 50 (in Chinese).

    [21] ZHANG Y, WANG H L, JIANG S Y, et al. Effect of different nitrogen concentrations on growth and antioxidant enzymes activity in Notopterygium incisum seedling in cultivation matrix [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24 (7): 38 (in Chinese).

    [22] XUE X L, ZHANG X H, YU J P, et al. Effect of heavy metal stress on the contents of ginsenosides [J]. Journal of Chinese Medicinal Materials, 2018, 41 (11): 2511 (in Chinese).

This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 08, Pages 1866-1872

April 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References