Inhibitory effect of wogonin on human colorectal cancer cell SW480 based on network pharmacology
(2.School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China 100029)
【Abstract】Wogonin is a main effective component of Radix Scutellariae, with a significant anti-cancer activity. Recently, extensive studies have focused on the anti-cancer pharmacological effects of wogonin, but there were still a few studies on its molecular mechanism. Therefore, the molecular targets of its anti-cancer activity were still unclear. In this study, network pharmacology was applied to investigate the potential targets and molecular pathway of wogonin in inhibiting the growth of colorectal cancer. It indicated that Wnt/β-catenin was a key pathway of wogonin on colorectal cancer. Then, studies on pharmacology and molecular mechanism were performed according to network pharmacological results. Pharmacological results revealed that wogonin inhibited significantly the proliferation of SW480 (P < 0.001), with concentration-dependent regularity in the range of 12.5–50 μmol·L−1. Additionally, wogonin could arrest SW480 cells in G1 phase. Western blot was used to investigate the effect of wogonin on four characteristic proteins of Wnt/β-catenin pathway. CTNNB1 (β-catenin), BIRC5 (survivin) and GSK3B were down-regulated significantly, while the expression level of BAX was up-regulated (P < 0.05). In conclusion, wogonin could inhibit the proliferation of SW480 cells through the Wnt/β-catenin pathway. The characteristic proteins CTNNB1 (β-catenin), BIRC5 (survivin), GSK3B and BAX were identified as the potential targets. This study illuminated the anti-cancer molecular mechanism and drug targets of wogonin, which provided a theoretical basis for anti-colon cancer drug discovery and clinical application.
【Keywords】 wogonin; colorectal cancer; network pharmacology; Wnt/β-catenin pathway;
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