Study on revision of quality standard of Sophorae Flavescentis Radix and its processed slices

FANG Yun-ge1,2 ZHU Hou-da2,3 LIU Xiao-qian2 CHEN Liang-mian2 MENG Chen-xiao-ning2 ZHANG Dong2 GAO Hui-min2 WANG Zhi-min2

(1.Tianjin University of Traditional Chinese Medicine, Tianjin, China 300193)
(2.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China 100700)
(3.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China 450008)

【Abstract】Sophorae Flavescentis Radix is prepared from the root of Sophora flavescens. The comprehensive knowledge of the effective components in S. flavescens makes its quality standard improvement more possible. TLC identification method for main flavonoids and alkaloids in one test was established using GF254 thin layer plate and the lower solution of chloroform-methanol-water-formic acid(4:2:1:0.6) as developing solvent. The quantitative method of marker alkaloids was revised including the simplified sample preparation procedure and the chromatographic conditions. The determination of four alkaloids in the samples indicated that 32 batches of tested samples were qualified ones. The total oxymatrine and matrine, total oxysophocarpine and sophocarpine, as well as total amount of four tested components in the different samples were 1.08%-2.55%, 0.369%-0.860%, 1.67%-3.40%, respectively. There was a significantly positive correlation between oxymatrine and oxysophocarpine. Total oxymatrine and matrine had same correlation with total oxysophocarpine and sophocarpine. The moisture content and extractive in 32 batches of samples fulfilled the require of not more than 11.0% and not less than 20.0%, respectively. Thirteen tested pesticides were not detected in 12 batches of samples. The present study provided the evidence for the revision of quality standard of Sophora Flavescentis Radix.

【Keywords】 Sophorae Flavescentis Radix; alkaloids; flavonoids; TLC identification; content determination; quality standard;

【DOI】

【Funds】 National Key R&D Program Project “Modernization of Traditional Chinese Medicine” (Grant 2017YFC1701900) National Standardization Project of Traditional Chinese Medicine (Grant ZYBZH-Y-JIN-34) Drug Standard Improvement Project of the Chinese Pharmacopoeia Commission in 2018 (Grant 2018Z064)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 08, Pages 1756-1763

April 2020

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Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References