Determination of five steroidal saponins in Rhizoma Paridis and its adulterants as well as consideration on its quantitative method described in Chinese Pharmacopoeia (2015 edition)

JU Bo-ya1 ZHU Hou-da1,2 LI Yan-min1 WANG Rui3 CHEN Liang-mian1 GAO Hui-min1 WANG Zhi-min1

(1.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
(2.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, Henan, China 450008)
(3.Shanxi University of Chinese Medicine, Taiyuan, Shanxi, China 030619)

【Abstract】Rhizoma Paridis was prepared from the dried rhizoma of Paris polyphylla var. yunnanensis or P. polyphylla var. chinensis. For the improvement of the quality standard of Rhizoma Paridis described in Chinese Pharmacopoeia (2015 edition), it is proposed that the quality marker no longer contains polyphyllin Ⅵ, and instead, polyphyllin H is an alternative for the quantitative analysis. To determine polyphyllin Ⅰ, Ⅱ, H and Ⅶ in the Rhizoma Paridis samples collected from the different growing area in China, HPLC method was established using the same chromatographic conditions as those for simultaneous determination of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ described in Chinese Pharmacopoeia (2015 edition). The methodology validation indicated that there was a good linearity among the ranges of 0.006 48–0.828, 0.006 52–0.834, 0.006 17–0.790, 0.006 31–0.808 g·L−1 for polyphyllin Ⅰ, Ⅱ, H and Ⅶ, respectively. The average recoveries of four components were 100.2%–101.4%, with RSD less than 3.5%. The total amounts of polyphyllin Ⅰ, Ⅱ, H and Ⅶ in the analyzed samples of P. polyphylla var. chinensis and P. polyphylla var. yunnanensis ranged from 0.050 9% to 3.99% and from 0.115% to 3.23%, respectively. In the tested samples collected from other Paris plants, there was high content of steroidal saponins in the samples of P. fargesii and P. forrestii, low content in the samples of P. polyphylla var. stenophylla, P. delavayi and P. thibetica, and almost not occurrence in the sample of P. mairei. As a representative adulterant of Rhizoma Paridis processed slices, 7 batches of Trillium samples contained high amount of polyphyllin Ⅵ and did not have polyphyllin H. Based on the present investigation, it is recommended that polyphyllin H together with polyphyllin Ⅰ, Ⅱ and Ⅶ are suitable for the improvement of quality standard of Rhizoma Paridis and the total amount of four components are not less than 0.80%.

【Keywords】 Rhizoma Paridis; Paris polyphylla var. yunnanensis; Paris polyphylla var. chinensis; Paris fargesii; Trillium tschonoskii/T. kamtschaticum; steroidal saponins; quality standard;

【DOI】

【Funds】 National Key R&D Program of China “Modernization of Chinese Medicine” (2017YFC1701900) Chinese Medicine Standardization Project of China (ZYBZH-Y-FJ-09) Special Funding Project for Research in Chinese Medicine Industry (201507002) Chinese Pharmacopoeia Commission 2018 Drug Standard Improvement Project (2018Z005)

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(Translated by ZHAO B)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 08, Pages 1745-1755

April 2020

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Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References