Determination of five steroidal saponins in Rhizoma Paridis and its adulterants as well as consideration on its quantitative method described in Chinese Pharmacopoeia (2015 edition)
(2.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, Henan, China 450008)
(3.Shanxi University of Chinese Medicine, Taiyuan, Shanxi, China 030619)
【Abstract】Rhizoma Paridis was prepared from the dried rhizoma of Paris polyphylla var. yunnanensis or P. polyphylla var. chinensis. For the improvement of the quality standard of Rhizoma Paridis described in Chinese Pharmacopoeia (2015 edition), it is proposed that the quality marker no longer contains polyphyllin Ⅵ, and instead, polyphyllin H is an alternative for the quantitative analysis. To determine polyphyllin Ⅰ, Ⅱ, H and Ⅶ in the Rhizoma Paridis samples collected from the different growing area in China, HPLC method was established using the same chromatographic conditions as those for simultaneous determination of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ described in Chinese Pharmacopoeia (2015 edition). The methodology validation indicated that there was a good linearity among the ranges of 0.006 48–0.828, 0.006 52–0.834, 0.006 17–0.790, 0.006 31–0.808 g·L−1 for polyphyllin Ⅰ, Ⅱ, H and Ⅶ, respectively. The average recoveries of four components were 100.2%–101.4%, with RSD less than 3.5%. The total amounts of polyphyllin Ⅰ, Ⅱ, H and Ⅶ in the analyzed samples of P. polyphylla var. chinensis and P. polyphylla var. yunnanensis ranged from 0.050 9% to 3.99% and from 0.115% to 3.23%, respectively. In the tested samples collected from other Paris plants, there was high content of steroidal saponins in the samples of P. fargesii and P. forrestii, low content in the samples of P. polyphylla var. stenophylla, P. delavayi and P. thibetica, and almost not occurrence in the sample of P. mairei. As a representative adulterant of Rhizoma Paridis processed slices, 7 batches of Trillium samples contained high amount of polyphyllin Ⅵ and did not have polyphyllin H. Based on the present investigation, it is recommended that polyphyllin H together with polyphyllin Ⅰ, Ⅱ and Ⅶ are suitable for the improvement of quality standard of Rhizoma Paridis and the total amount of four components are not less than 0.80%.
【Keywords】 Rhizoma Paridis; Paris polyphylla var. yunnanensis; Paris polyphylla var. chinensis; Paris fargesii; Trillium tschonoskii/T. kamtschaticum; steroidal saponins; quality standard;
(Translated by ZHAO B)
 Chinese Pharmacopoeia Commission. Chinese pharmacopoeia. Volume Ⅰ [S]. Beijing: China Medical Science Press, 2015: 260 (in Chinese).
 MAN S L, WANG Y L, LI Y Y, et al. Phytochemistry, pharmacology, toxicology, and structure–cytotoxicity relationship of Paridis Rhizome saponin [J]. Chin Herb Med, 2013, 5 (1): 33.
 LIU Z, GAO W Y, JING S S, et al. Correlation among cytotoxicity, hemolytic activity and the composition of steroidal saponins from Paris L. [J]. J Ethopharmacol, 2013, 149: 422.
 GUAN X, LI R S, DUAN B Z, et al. Advances in research on chemical constituents and pharmacological effects of Paris genus and prediction and analysis of quality markers [J]. Chinese Traditional and Herbal Drugs, 2019, 50 (19): 4838–4852 (in Chinese).
 JU B Y. Study on the quality standard and grade specification of Paridis Rhizome, and its processed slices [D]. Taiyuan: Shanxi University of Chinese Medicine, 2020 (in Chinese).
 LI Y, HE J, ZHAO T Z, et al. Determination of six saponins in Paris polyphylla var. yunnanens from different habitats by HPLC [J]. Chinese Traditional Patent Medicine, 2012, 34 (1): 113–116 (in Chinese).
 ZHANG S S, LIU X, WANG J F, et al. Determination of seven steroidal saponins in Paridis Rhizoma and polygerm varieties from different regions in Yunnan province by UPLC and establishment of fingerprint [J]. Chinese Traditional and Herbal Drugs, 2016, 47 (23): 4257–4263 (in Chinese).
 CHEN T Z, WEN F Y, ZHANG T, et al. Evaluation of saponins in Paris polyphylla var. chinensis from twenty-one growing areas [J]. Chinese Traditional Patent Medicine, 2017, 39 (11): 2345–2350 (in Chinese).
 XIAO C, RAO W W, WU M, et al. Analysis of polyphyllins and discussion about TLC identification method for 20 batches of Paridis Rhizoma samples [J]. Drug Standards of China, 2016, 17 (6): 403–405 (in Chinese).
 JU B Y, LI Y M, ZHU H D, et al. Improving quality standard of processed slices of Paridis Rhizoma of Chinese Pharmacopoeia [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019, 25 (19): 93–101 (in Chinese).
 GAO Y, LI D W, GUO L N, et al. Quality analysis and study on Paridis Rhizoma pieces based on national drug evaluation sampling inspection [J]. Chinese Pharmaceutical Affairs, 2019, 33 (7): 754–759 (in Chinese).
 WEN F Y, YIN H X, CHEN C, et al. Chemical characteristics of saponins from Paris fargesii var. brevipetala and cytotoxic activity of its main ingredient, paris saponin H [J]. Fitoterapia, 2012, 83: 627.
 LIANG Y Y, LIU Z, GAO W Y, et al. Determination of nine steroidal saponins in Paris polyphylla from different areas of Guizhou province by HPLC [J]. China Journal of Chinese Materia Medica, 2012, 37 (15): 2309–2312 (in Chinese).
 HUANG Y Y, LIU D H, et al. Determination of eight steroidal saponins in 15 kinds of genus Paris [J]. China Journal of Chinese Materia Medica, 2017, 42 (18): 3443–3451 (in Chinese).
 ZAN K, GAO Y M, CUI G, et al. Simultaneous determination of eleven steroidal saponins in cultivated Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. by UPLC [J]. Chinese Journal of Pharmaceutical Analysis, 2017, 37 (9): 1572–1577 (in Chinese).
 WU W Y, GUO D A. Strategies for elaboration of comprehensive quality standard system on traditional Chinese medicine [J]. China Journal of Chinese Materia Medica, 2014, 39 (3): 351–356 (in Chinese).
 Li H. Plants in the genus Paris [M]. Beijing: Science Press, 1998 (in Chinese).
 Gansu Medical Products Administration. Chinese medicinal standards of Gansu province [S]. Lanzhou: Gansu Culture Press, 2009: 76 (in Chinese).
 Zhejiang Medical Products Administration. Chinese medicinal standards of Zhejiang province [S]. 2017: 37 (in Chinese).
 Sichuan Medical Products Administration. Tibetan medicinal standards of Sichuan province [S]. Chengdu: Sichuan Science & Technology Press, 2014: 152, 180 (in Chinese).
 YANG Y J, SUN X G, et al. Determination of three saponins in rhizoma and fibrous root of Trillium tschonoskii and Trillium kamtschaticum [J]. China Journal of Chinese Materia Medica, 2017, 42 (6): 1146–1151 (in Chinese).