Studies on quality standards of Poria

TIAN Shuang-shuang1,2 ZHAO Xiao-mei1 LIU Yong1,2 GE Xiao-hong1 FENG Wei-hong1 CHEN Liang-mian1 YAN Li-hua1 WANG Zhi-min1

(1.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
(2.Henan University of Chinese Medicine, Zhengzhou, Henan, China 450000)

【Abstract】In order to provide scientific recommendations for the revision of the quality standards of Poria in Chinese Pharmacopoeia (2020 edition), a series of experiments were carried out to improve the quality standards of Poria. TLC methods were established to identify Poria by using pachymic acid, dehydrotumulosic acid and Chinese medicinals as reference substances. The contents of water, total ash, pesticide residues, heavy metals and deleterious element, mycotoxins, sulfur dioxide residues and ethanol-soluble extract of medicinal materials and processed slices of Poria were determined according to the methods recorded in the volume Ⅳ of Chinese Pharmacopoeia (2015 edition). An HPLC method was developed for the determination of pachymic acid and dehydropachymic acid. The contents of polysaccharides were determined by spectrophotometry using anhydro-D-glucose as a reference substance. The quality standards were established on the basis of the research results, in which the [assay] was added, and the [identification] and [tests] were revised when compared with Chinese Pharmacopoeia (2015 edition). The established methods are simple, specific, repeatable, and suitable for the quality evaluation of Poria.

【Keywords】 Poria; quality standards; pachymic acid; dehydropachymic acid; Poria cocos polysaccharide; TLC; HPLC;

【DOI】

【Funds】 National Drug Standards Improvement Project of the Chinese Pharmacopoeia Commission (2019Z019, 2018Z005) National Traditional Chinese Medicine Standardization Project (ZYBZH-C-JL-24).

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(Translated by SU JB)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 08, Pages 1734-1744

April 2020

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Article Outline

Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References