Studies on quality standards of Poria
(2.Henan University of Chinese Medicine, Zhengzhou, Henan, China 450000)
【Abstract】In order to provide scientific recommendations for the revision of the quality standards of Poria in Chinese Pharmacopoeia (2020 edition), a series of experiments were carried out to improve the quality standards of Poria. TLC methods were established to identify Poria by using pachymic acid, dehydrotumulosic acid and Chinese medicinals as reference substances. The contents of water, total ash, pesticide residues, heavy metals and deleterious element, mycotoxins, sulfur dioxide residues and ethanol-soluble extract of medicinal materials and processed slices of Poria were determined according to the methods recorded in the volume Ⅳ of Chinese Pharmacopoeia (2015 edition). An HPLC method was developed for the determination of pachymic acid and dehydropachymic acid. The contents of polysaccharides were determined by spectrophotometry using anhydro-D-glucose as a reference substance. The quality standards were established on the basis of the research results, in which the [assay] was added, and the [identification] and [tests] were revised when compared with Chinese Pharmacopoeia (2015 edition). The established methods are simple, specific, repeatable, and suitable for the quality evaluation of Poria.
【Keywords】 Poria; quality standards; pachymic acid; dehydropachymic acid; Poria cocos polysaccharide; TLC; HPLC;
(Translated by SU JB)
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