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基于质量常数评价方法的山豆根饮片等级评价研究

赵桉熠1,2 刘艳2 石佳2 倪凤燕2 刘安2 张爱平1

(1.山西医科大学药学院, 山西太原 030001)
(2.中国中医科学院中药研究所, 北京 100700)

【摘要】以传统性状鉴别与现代科技手段结合的中药质量常数饮片评价方法划分山豆根饮片等级。应用中药质量常数评价方法从饮片外观形态与指标含量测定入手,通过计算得到不同来源批次的山豆根饮片质量常数和百分质量常数等数值,探讨山豆根饮片等级划分情况。结果表明,所收集的15批山豆根饮片质量常数介于0.004~0.063;以百分质量常数≥80%为一等饮片,≥50%且<80%为二等饮片,<50%为三等饮片,则所收集的山豆根饮片中,其质量常数≥0.050为一等饮片;质量常数≥0.032且<0.050为二等饮片;<0.032为三等饮片。通过研究将15批山豆根饮片成功划分为一等1批,二等为4批,三等10批。中药质量常数评价方法科学、客观、其操作简单易行,将其应用于山豆根饮片等级划分,结果合理,具有较好的推广性,有助于促进中药市场对山豆根饮片的分类分级,为该饮片优质应用提供思路。

【关键词】 山豆根;质量常数;百分质量常数;苦参碱;氧化苦参碱;等级评价;

【DOI】

【基金资助】 国家自然科学基金项目(81703945); 国家中医药管理局中药标准化项目(ZYBZH-Y-JX-27); 北京市科委“十病十药”研发专项(Z171100001717029);

Grade evaluation of Sophorae Tonkinensis Radix et Rhizoma based on quality constant evaluation method

ZHAO An-yi1,2 LIU Yan2 SHI Jia2 NI Feng-yan2 LIU An2 ZHANG Ai-ping1

(1.School of Pharmacy, Shanxi Medical University, Taiyuan, China 030001)
(2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)

【Abstract】The traditional Chinese medicine(TCM) quality constant herbal slices evaluation method was applied to evaluate the grade of Sophorae Tonkinensis Radix et Rhizoma based on the combination of traditional character identification and modern scientific and technological methods. The TCM quality constant evaluation method was used to determine the appearance and index content of medicinal slices, calculate the quality constant and percentile quality constant of Sophorae Tonkinensis Radix et Rhizoma from different sources, and discuss their classification. The quality constants of 15 batches of Sophorae Tonkinensis Radix et Rhizoma slices were between 0.004 and 0.063. The slices with the percentage quality constant ≥80% were classified as the first grade; those with the percentage quality constant ≥50% and <80% were classified as the second grade; and those with the percentage quality constant <50% were classified as the third grade. Then the slices with the quality constant ≥0.050 were classified as the first grade; those with the quality constant ≥0.032 and <0.050 were classified as the second grade; and those with the quality constant <0.032 were classified as the third grade. According to the results, the 15 batches of Sophorae Tonkinensis Radix et Rhizoma slices were divided into 1 batch of the first grade, 4 batches of the second grade, and 10 batches of the third grade. The quality constant evaluation method established is scientific, objective, simple and feasible. The application of the method in Sophorae Tonkinensis Radix et Rhizoma slices has reasonable results, which is helpful to promote the classification of Sophorae Tonkinensis Radix et Rhizoma and promotes the high-quality application of Sophorae Tonkinensis Radix et Rhizoma.

【Keywords】 Sophorae Tonkinensis Radix et Rhizoma; quality constant; percentage quality constant; matrine; oxymatrine; grade evaluation;

【DOI】

【Funds】 National Natural Science Foundation of China (Grant No. 81703945); Standardization Project of Traditional Chinese Medicine of the National Administration of Traditional Chinese Medicine (Grant No. ZYBZH-Y-JX-27); Special Research and Development Project of “Ten Diseases and Ten Drugs” of Beijing Municipal Science & Technology Commission (Grant No. Z171100001717029);

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    References

    [1] Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia. Vol. One [S]. Beijing: China Medical Science Press, 2015: 25 (in Chinese).

    [2] YAO Z Q, ZHU H, WANG G F. Anti-tumor effect of total alkaloids of Radix Sophorue Tonldnensis [J]. Journal of Nanjing University of Traditional Chinese Medicine, 2005, 21 (4): 253 (in Chinese).

    [3] LAN Y S, YANG R Y, LI Y, et al. The progress of research on chemical composition and pharmacological activity in Sophora Tonkinensis[J]. Journal of Chuzhou University, 2010, 12 (2): 48 (in Chinese).

    [4] XU J, MA C Y, WEN L M, et al. Research progress of Sophora tonkinensis [J]. Chinese Archives of Traditional Chinese Medicine, 2012, 30 (11): 2428 (in Chinese).

    [5] LI Q P, MIAO J H, SONG Z J, et al. Research on Non-alkaloids of Sophora tonkinensis[J]. Modern Chinese Medicine, 2018, 20 (9): 1169 (in Chinese).

    [6] WANG J M, CUI Y. Research progress in chemical components, pharmacological effectiveness and toxicity of Radix et Rhizoma Sophorae tonkinensis [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2011, 17 (4): 229-232 (in Chinese).

    [7] DING P L, CHEN D F. 中药山豆根及其制剂的药理作用、临床应用与不良反应[J]. Chinese Journal of Clinical Pharmacy, 2003, 12 (5): 315 (in Chinese).

    [8] CHENG Q, ZOU Q W, WANG J F, et al. Simultaneous determination of four alkaloids in Radix et Rhizoma Sophorae Tonkinensis by HPLC and cluster analysis [J]. China Pharmaceuticals, 2017, 26 (17): 10 (in Chinese).

    [9] WU L Q, CHEN Q Q, PAN J H, et al. Drug Inspection. Analysis on the quality of soybean root in the 33 lots of city [J]. Journal of North Pharmacy, 2019, 16 (8): 12 (in Chinese).

    [10] YANG W Y, MA L J. HPLC法同时测定山豆根中苦参碱和氧化苦参碱 [J]. Chinese Traditional and Herbal Drugs, 2004, 35 (3): 329 (in Chinese).

    [11] HAN F M, WANG L X, CHEN Y, et al. Simultaneous determination of seven alkaloids and three flavonoids in Sophorae Tonkinensis Radix et Rhizoma by HPLC[J]. China Journal of Chinese Materia Medica, 2016, 41 (24): 4628 (in Chinese).

    [12] National Administration of Traditional Chinese Medicine. 中华本草 [M]. Shanghai: Shanghai Scientific & Technical Publishers, 1999 (in Chinese).

    [13] DENG Z, JIAO M J, ZHANG J, et al. Application of relative quality constant in grades evaluation of Glycyrrizae Radix et Rhizome pieces[J]. China Journal of Chinese Materia Medica, 2017, 42 (13): 2492 (in Chinese).

    [14] DENG Z, ZHANG J, JIAO M J, et al. Grades evaluation of Scutellariae Radix slices based on quality constant [J]. China Journal of Chinese Materia Medica, 2017, 42 (9): 1673 (in Chinese).

    [15] DENG Z, JIAO M J, ZHANG J, et al. Grades evaluation of Phellodendri chinensis cortex pieces based on quality constant[J]. China Journal of Chinese Materia Medica, 2017, 42 (17): 3356 (in Chinese).

    [16] SHI J, SHEN L, DENG Z, et al. Grades evaluation of Scrophulariae Radix pieces based on quality constant [J]. China Journal of Chinese Materia Medica, 2019, 44 (9): 1744 (in Chinese).

    [17] SHI J, DENG Z, SHEN L, et al. Grading of Gastrodia elata pieces based on quality constant [J]. China Journal of Chinese Materia Medica, 2019, 44 (9): 1750 (in Chinese).

    [18] DENG Z, SHI J, SHEN L, et al. Research on grades evaluation of Glycyrrizae Radix et Rhizoma Praeparata Cum Melle based on Chinese medicine quality constant[J]. China Journal of Chinese Materia Medica, 2019, 44 (9): 1739 (in Chinese).

    [19] SANG X X. Liver protection and antiviral effect and immunological mechanism of the main active ingredients of Sophora Tonkinensis Gagnep [D]. Chengde: Chengde Medical College, 2018 (in Chinese).

    [20] QIAN L W, DAI W H, ZHOU G Q, et al. 苦参及山豆根主要生物碱镇痛抗炎作用研究 [J]. Chinese Traditional Patent Medicine, 2012, 34 (8): 1593 (in Chinese).

    [21] YANG F. Study on the Extraction and Separation of the Alkaloids from Radix Sophorae Tonkinensis and Its Anti-oxidative and Anti-tumor Activity [D]. Changchun: Changchun Normal University, 2012 (in Chinese).

    [22] China Association of Chinese Medicine. 中药材商品规格等级山豆根 [S]. 2018.T/CACM 1021.112-2018 (in Chinese).

    [23] YANG X, XIA D S, GAO J C, et al. Literature Analysis of 578 Cases of Adverse Drug Reactions Induced by Sophora tonkinensis Gagnep [J]. Chinese Journal of Pharmacovigilance, 2017, 14 (4): 235 (in Chinese).

    [24] TIAN X S. Research Progress on Toxicity of Alkaloids in Sophorae Tonkinensis Radix et Rhizoma [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2016, 22 (6): 230 (in Chinese).

    [25] Wang X P, Chen J T, Xiao Q, et al. Intoxication of Radix Sophorae Subprostrate: from human to animal [J]. Nature Magazine, 2002, 24 (5): 286 (in Chinese).

    [26] SHI J, JIAO M J, DENG Z, et al. High quality and superior effect of Dachuanxiong Formula based on quality constant grading evaluation technology of Chinese medicine pieces [J]. China Journal of Chinese Materia Medica, 2019, 44 (09): 1755 (in Chinese).

This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 07, Pages 1664-1669

April 2020

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References