(2.修正药业集团股份有限公司, 吉林通化 134000)
【摘要】该文通过UPLC-Q-TOF-MS指认了泽泻水提物中核苷、氨基酸等10个成分。首次建立了泽泻药材中核苷类成分的HPLC指纹图谱，结合指纹图谱相似度评价、聚类分析比较核苷类成分在不同产地泽泻药材中的差异。同时首次建立泽泻药材中5种主要核苷(胞苷、尿苷、腺嘌呤、鸟苷、腺苷)的含量测定方法。结果显示，川泽泻与建泽泻的相似度在0.96以上，广泽泻相似度小于0.87;聚类分析中川泽泻与建泽泻聚为一组，广泽泻聚为另一组，提示在核苷类成分上川泽泻与建泽泻较为相似，与广泽泻差异较大。含量测定结果显示，川泽泻与建泽泻上述5种成分质量分数总和为0.81～1.30 mg·g-1，广泽泻为0.35～0.50 mg·g-1。在川泽泻与建泽泻药材中含量由高到低依次为鸟苷>胞苷>尿苷>腺嘌呤>腺苷;在广泽泻中依次为尿苷>腺嘌呤>鸟苷>胞苷>腺苷。川泽泻与建泽泻中上述5种化合物的含量均高于广泽泻;川泽泻与建泽泻相比，除腺嘌呤外，均为川泽泻略高于建泽泻。研究结果为进一步揭示泽泻药效物质及药材质量的全面控制提供参考。
【基金资助】 国家中药标准化项目(ZYBZH-C-JL-24); 国家药典委员会标准制修订研究课题(2018Z079);
Qualitative and quantitative research on nucleosides of Rhizoma Alismatis from different regions
(2.Xiuzheng Pharmaceutical Group Co., Ltd., Tonghua, Jilin Province, China 134000)
【Abstract】Ten compounds, including nucleosides and amino acids were identified by UPLC-Q-TOF-MS. HPLC fingerprints on these compounds in Rhizoma Alismatis were established for the first time. The comparisons of Rhizoma Alismatis from different regions were conducted by the similarity evaluation and hierarchical cluster analysis (HCA). Meanwhile, the HPLC-DAD method for the content determination of five nucleosides was also established. The results showed that the similarities of Rhizoma Alismatis collected from Sichuan and Fujian provinces were above 0.96, whereas they were less than 0.87 in those from Guangxi Province. The results of HCA showed the samples from Sichuan and Fujian were gathered in the same group, all samples from Guangxi in another group, which indicated the similarities between the samples from Sichuan and Fujian in nucleosides and they were different from the samples from Guangxi. The total content of five nucleosides was revealed, of which the samples from Sichuan and Fujian were 0.81–1.30 mg·g−1 followed a descending order of vernine > cytidine > uridine > adenine > adenosine, and from Guangxi were 0.35–0.50 mg·g−1 with the sequences of uridine > adenine > vernine > cytidine > adenosine. The nucleosides content of the samples from Sichuan and Fujian was both higher than that from Guangxi. For the samples from Sichuan and Fujian, the former was slightly higher, except for adenine. These results would be helpful to reveal the bioactive constituents in aqueous extract and provided important evidences for the quality control of Rhizoma Alismatis.
【Keywords】 Rhizoma Alismatis; nucleosides; fingerprint; content determination;
【Funds】 National Chinese Medicine Standardization Project (ZYBZH-C-JL-24); Research Project on Standard Formulation and Revision of the Chinese Pharmacopoeia Commission (2018Z079);
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