Side-by-side Chinese-English

不同产地泽泻药材中核苷类成分定性定量分析

刘珊珊1 刘晓谦1 田双双1 朱晶晶1 高慧敏1 闫利华1 王智民1 高陆2

(1.中国中医科学院中药研究所中药质量控制技术国家工程实验室, 北京 100700)
(2.修正药业集团股份有限公司, 吉林通化 134000)

【摘要】该文通过UPLC-Q-TOF-MS指认了泽泻水提物中核苷、氨基酸等10个成分。首次建立了泽泻药材中核苷类成分的HPLC指纹图谱,结合指纹图谱相似度评价、聚类分析比较核苷类成分在不同产地泽泻药材中的差异。同时首次建立泽泻药材中5种主要核苷(胞苷、尿苷、腺嘌呤、鸟苷、腺苷)的含量测定方法。结果显示,川泽泻与建泽泻的相似度在0.96以上,广泽泻相似度小于0.87;聚类分析中川泽泻与建泽泻聚为一组,广泽泻聚为另一组,提示在核苷类成分上川泽泻与建泽泻较为相似,与广泽泻差异较大。含量测定结果显示,川泽泻与建泽泻上述5种成分质量分数总和为0.81~1.30 mg·g-1,广泽泻为0.35~0.50 mg·g-1。在川泽泻与建泽泻药材中含量由高到低依次为鸟苷>胞苷>尿苷>腺嘌呤>腺苷;在广泽泻中依次为尿苷>腺嘌呤>鸟苷>胞苷>腺苷。川泽泻与建泽泻中上述5种化合物的含量均高于广泽泻;川泽泻与建泽泻相比,除腺嘌呤外,均为川泽泻略高于建泽泻。研究结果为进一步揭示泽泻药效物质及药材质量的全面控制提供参考。

【关键词】 泽泻;核苷;指纹图谱;含量测定;

【DOI】

【基金资助】 国家中药标准化项目(ZYBZH-C-JL-24); 国家药典委员会标准制修订研究课题(2018Z079);

Qualitative and quantitative research on nucleosides of Rhizoma Alismatis from different regions

LIU Shan-shan1 LIU Xiao-qian1 TIAN Shuang-shuang1 ZHU Jing-jing1 GAO Hui-min1 YAN Li-hua1 WANG Zhi-min1 GAO Lu2

(1.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
(2.Xiuzheng Pharmaceutical Group Co., Ltd., Tonghua, Jilin Province, China 134000)

【Abstract】Ten compounds, including nucleosides and amino acids were identified by UPLC-Q-TOF-MS. HPLC fingerprints on these compounds in Rhizoma Alismatis were established for the first time. The comparisons of Rhizoma Alismatis from different regions were conducted by the similarity evaluation and hierarchical cluster analysis (HCA). Meanwhile, the HPLC-DAD method for the content determination of five nucleosides was also established. The results showed that the similarities of Rhizoma Alismatis collected from Sichuan and Fujian provinces were above 0.96, whereas they were less than 0.87 in those from Guangxi Province. The results of HCA showed the samples from Sichuan and Fujian were gathered in the same group, all samples from Guangxi in another group, which indicated the similarities between the samples from Sichuan and Fujian in nucleosides and they were different from the samples from Guangxi. The total content of five nucleosides was revealed, of which the samples from Sichuan and Fujian were 0.81–1.30 mg·g−1 followed a descending order of vernine > cytidine > uridine > adenine > adenosine, and from Guangxi were 0.35–0.50 mg·g−1 with the sequences of uridine > adenine > vernine > cytidine > adenosine. The nucleosides content of the samples from Sichuan and Fujian was both higher than that from Guangxi. For the samples from Sichuan and Fujian, the former was slightly higher, except for adenine. These results would be helpful to reveal the bioactive constituents in aqueous extract and provided important evidences for the quality control of Rhizoma Alismatis.

【Keywords】 Rhizoma Alismatis; nucleosides; fingerprint; content determination;

【DOI】

【Funds】 National Chinese Medicine Standardization Project (ZYBZH-C-JL-24); Research Project on Standard Formulation and Revision of the Chinese Pharmacopoeia Commission (2018Z079);

Download this article
    References

    [1] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Vol. Ⅰ [S]. Beijing: China Medical Science Press, 2015: 229 (in Chinese).

    [2] CAI D L, YANG C Z, WEN X P, et al. Resource investigation and evaluation of character for Alismatis Rhizoma [J]. Modern Chinese Medicine, 2016, 18 (2): 193 (in Chinese).

    [3] SHU Z, PU J, CHEN L, et al. Alisma orientale: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine [J]. Am J Chin Med, 2016, 44 (2): 227.

    [4] ZHANG L L, XU W, XU Y L, et al. Therapeutic potential of Rhizoma Alismatis: a review on ethnomedicinal application, phytochemistry, pharmacology, and toxicology [J]. Ann N Y Acad Sci, 2017, 1401 (1): 90.

    [5] WU S S. 泽泻的药学与临床研究 [M]. Beijing: China Press of Traditional Chinese Medicine, 2009 (in Chinese).

    [6] TAI Y N, WU X, FAN L M, et al. Simultaneous determination of sixteen components in Alismatis Rhizoma by UPLC-MS/MS [J]. Chinese Journal of Pharmaceutical Analysis, 2018, 38 (8): 1337 (in Chinese).

    [7] WU J, YANG W, PAN H, et al. Geographic impact evaluation of the quality of Alismatis Rhizoma by untargeted metabolomics and quantitative assay [J]. J Sep Sci, 2018, 41 (4): 839.

    [8] WU J X, XU M Y. The research progress in the myocardial protection effect of adenosine [J]. Chinese Journal of Cardiovascular Medicine, 2002, 7 (5): 364-366 (in Chinese).

    [9] HEADRICK J P, PEART J N, REICHELT M E, et al. Adenosine and its receptors in the heart: regulation, retaliation and adaptation [J]. Biochim Biophys Acta, 2011, 1808 (5): 1413.

    [10] PU Z J, ZHOU X T, LI G P, et al. Effect of adenosine on autophagy and proliferation of hepatocellular carcinoma cells [J]. Chinese Pharmacological Bulletin, 2018, 34 (4): 508 (in Chinese).

    [11] KINAHAN J J, KOWAL E P, GRINDEY G B. Biochemical and antitumor effects of the combination of thymidine and 1-beta-D-arabinofuranosylcytosine against leukemia L1210 [J]. Cancer Res, 1981, 41 (2): 445.

    [12] WANG D M, LIU J H, ZHOU J, et al. Inhibiting effect of adenosine on platelet activation in vitro [J]. Journal of Experimental Hematology, 2005, 13 (6): 1094 (in Chinese).

    [13] MORIMOTO C, STEINBERG A D, SCHLOSSMAN S F, et al. In vitro nucleoside specific immune response by lymphocytes from systemic lupus erythematosus [J]. J Clin Invest, 1983, 71 (5): 1402.

    [14] ZAN K, HUANG L L, GUO L N, et al. Comparative study on specific chromatograms and main nucleosides of cultivated and wild Cordyceps sinensis [J]. China Journal of Chinese Materia Medica, 2017, 42 (20): 3957 (in Chinese).

    [15] ZHOU H, CAO Y M, MIAO S, et al. Determination of 7 nucleosides in Shanghai Pheretima by HPLC [J]. Chinese Journal of Pharmaceutical Analysis, 2018, 38 (1): 97 (in Chinese).

    [16] ZHANG Q, ZHU Y H, WEN H M, et al. Simultaneous determination of eight nucleosides in scolopendra of different varieties and origins by HPLC-PDA [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23 (14): 57 (in Chinese).

    [17] WANG P Z, XIANG M R, LI C, et al. Simultaneous determination of nine nucleosides in the rhizome of Pinellia ternata from different origins and its counterfeit by HPLC [J]. Chinese Journal of Pharmaceutical Analysis, 2017, 37 (2): 212 (in Chinese).

    [18] MA P, WANG L, SUN N X, et al. HPLC-DAD同时测定太白贝母中9种核苷类成分的含量 [J]. Journal of Chinese Medicinal Materials, 2016, 9 (1): 124 (in Chinese).

    [19] ZHAO W L, HUANG X Q, XU W, et al. Simultaneous determination of 11 triterpenoids in Alismatis Rhizoma by RP-HPLC-DAD [J]. Chinese Traditional and Herbal Drugs, 2016, 47 (16): 2933 (in Chinese).

    [20] WU S S, CHEN J Y, HUANG Z H, et al.从植物分类学及分子生物学角度区分建泽泻与川泽泻 [C]. Kaili: The 8th National Seminar on Natural Medicine Resources, 2008 (in Chinese).

    [21] TAI Y N, WENG Y H, ZHANG S P, et al. Determination of seven ingredients of different grades Alismatis Rhizoma by QAMS method [J]. China Journal of Chinese Materia Medica, 2019, 44 (11): 2292 (in Chinese).

This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 45, No. 07, Pages 1558-1565

April 2020

Downloads:2

Share
Article Outline

Abstract

  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References