Qualitative and quantitative research on nucleosides of Rhizoma Alismatis from different regions
(2.Xiuzheng Pharmaceutical Group Co., Ltd., Tonghua, Jilin Province, China 134000)
【Abstract】Ten compounds, including nucleosides and amino acids were identified by UPLC-Q-TOF-MS. HPLC fingerprints on these compounds in Rhizoma Alismatis were established for the first time. The comparisons of Rhizoma Alismatis from different regions were conducted by the similarity evaluation and hierarchical cluster analysis (HCA). Meanwhile, the HPLC-DAD method for the content determination of five nucleosides was also established. The results showed that the similarities of Rhizoma Alismatis collected from Sichuan and Fujian provinces were above 0.96, whereas they were less than 0.87 in those from Guangxi Province. The results of HCA showed the samples from Sichuan and Fujian were gathered in the same group, all samples from Guangxi in another group, which indicated the similarities between the samples from Sichuan and Fujian in nucleosides and they were different from the samples from Guangxi. The total content of five nucleosides was revealed, of which the samples from Sichuan and Fujian were 0.81–1.30 mg·g−1 followed a descending order of vernine > cytidine > uridine > adenine > adenosine, and from Guangxi were 0.35–0.50 mg·g−1 with the sequences of uridine > adenine > vernine > cytidine > adenosine. The nucleosides content of the samples from Sichuan and Fujian was both higher than that from Guangxi. For the samples from Sichuan and Fujian, the former was slightly higher, except for adenine. These results would be helpful to reveal the bioactive constituents in aqueous extract and provided important evidences for the quality control of Rhizoma Alismatis.
【Keywords】 Rhizoma Alismatis; nucleosides; fingerprint; content determination;
 Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Vol. Ⅰ [S]. Beijing: China Medical Science Press, 2015: 229 (in Chinese).
 CAI D L, YANG C Z, WEN X P, et al. Resource investigation and evaluation of character for Alismatis Rhizoma [J]. Modern Chinese Medicine, 2016, 18 (2): 193 (in Chinese).
 SHU Z, PU J, CHEN L, et al. Alisma orientale: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine [J]. Am J Chin Med, 2016, 44 (2): 227.
 ZHANG L L, XU W, XU Y L, et al. Therapeutic potential of Rhizoma Alismatis: a review on ethnomedicinal application, phytochemistry, pharmacology, and toxicology [J]. Ann N Y Acad Sci, 2017, 1401 (1): 90.
 WU S S. 泽泻的药学与临床研究 [M]. Beijing: China Press of Traditional Chinese Medicine, 2009 (in Chinese).
 TAI Y N, WU X, FAN L M, et al. Simultaneous determination of sixteen components in Alismatis Rhizoma by UPLC-MS/MS [J]. Chinese Journal of Pharmaceutical Analysis, 2018, 38 (8): 1337 (in Chinese).
 WU J, YANG W, PAN H, et al. Geographic impact evaluation of the quality of Alismatis Rhizoma by untargeted metabolomics and quantitative assay [J]. J Sep Sci, 2018, 41 (4): 839.
 WU J X, XU M Y. The research progress in the myocardial protection effect of adenosine [J]. Chinese Journal of Cardiovascular Medicine, 2002, 7 (5): 364-366 (in Chinese).
 HEADRICK J P, PEART J N, REICHELT M E, et al. Adenosine and its receptors in the heart: regulation, retaliation and adaptation [J]. Biochim Biophys Acta, 2011, 1808 (5): 1413.
 PU Z J, ZHOU X T, LI G P, et al. Effect of adenosine on autophagy and proliferation of hepatocellular carcinoma cells [J]. Chinese Pharmacological Bulletin, 2018, 34 (4): 508 (in Chinese).
 KINAHAN J J, KOWAL E P, GRINDEY G B. Biochemical and antitumor effects of the combination of thymidine and 1-beta-D-arabinofuranosylcytosine against leukemia L1210 [J]. Cancer Res, 1981, 41 (2): 445.
 WANG D M, LIU J H, ZHOU J, et al. Inhibiting effect of adenosine on platelet activation in vitro [J]. Journal of Experimental Hematology, 2005, 13 (6): 1094 (in Chinese).
 MORIMOTO C, STEINBERG A D, SCHLOSSMAN S F, et al. In vitro nucleoside specific immune response by lymphocytes from systemic lupus erythematosus [J]. J Clin Invest, 1983, 71 (5): 1402.
 ZAN K, HUANG L L, GUO L N, et al. Comparative study on specific chromatograms and main nucleosides of cultivated and wild Cordyceps sinensis [J]. China Journal of Chinese Materia Medica, 2017, 42 (20): 3957 (in Chinese).
 ZHOU H, CAO Y M, MIAO S, et al. Determination of 7 nucleosides in Shanghai Pheretima by HPLC [J]. Chinese Journal of Pharmaceutical Analysis, 2018, 38 (1): 97 (in Chinese).
 ZHANG Q, ZHU Y H, WEN H M, et al. Simultaneous determination of eight nucleosides in scolopendra of different varieties and origins by HPLC-PDA [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23 (14): 57 (in Chinese).
 WANG P Z, XIANG M R, LI C, et al. Simultaneous determination of nine nucleosides in the rhizome of Pinellia ternata from different origins and its counterfeit by HPLC [J]. Chinese Journal of Pharmaceutical Analysis, 2017, 37 (2): 212 (in Chinese).
 MA P, WANG L, SUN N X, et al. HPLC-DAD同时测定太白贝母中9种核苷类成分的含量 [J]. Journal of Chinese Medicinal Materials, 2016, 9 (1): 124 (in Chinese).
 ZHAO W L, HUANG X Q, XU W, et al. Simultaneous determination of 11 triterpenoids in Alismatis Rhizoma by RP-HPLC-DAD [J]. Chinese Traditional and Herbal Drugs, 2016, 47 (16): 2933 (in Chinese).
 WU S S, CHEN J Y, HUANG Z H, et al.从植物分类学及分子生物学角度区分建泽泻与川泽泻 [C]. Kaili: The 8th National Seminar on Natural Medicine Resources, 2008 (in Chinese).
 TAI Y N, WENG Y H, ZHANG S P, et al. Determination of seven ingredients of different grades Alismatis Rhizoma by QAMS method [J]. China Journal of Chinese Materia Medica, 2019, 44 (11): 2292 (in Chinese).