Qualitative and quantitative research on nucleosides of Rhizoma Alismatis from different regions

LIU Shan-shan1 LIU Xiao-qian1 TIAN Shuang-shuang1 ZHU Jing-jing1 GAO Hui-min1 YAN Li-hua1 WANG Zhi-min1 GAO Lu2

(1.National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)
(2.Xiuzheng Pharmaceutical Group Co., Ltd., Tonghua, Jilin Province, China 134000)

【Abstract】Ten compounds, including nucleosides and amino acids were identified by UPLC-Q-TOF-MS. HPLC fingerprints on these compounds in Rhizoma Alismatis were established for the first time. The comparisons of Rhizoma Alismatis from different regions were conducted by the similarity evaluation and hierarchical cluster analysis (HCA). Meanwhile, the HPLC-DAD method for the content determination of five nucleosides was also established. The results showed that the similarities of Rhizoma Alismatis collected from Sichuan and Fujian provinces were above 0.96, whereas they were less than 0.87 in those from Guangxi Province. The results of HCA showed the samples from Sichuan and Fujian were gathered in the same group, all samples from Guangxi in another group, which indicated the similarities between the samples from Sichuan and Fujian in nucleosides and they were different from the samples from Guangxi. The total content of five nucleosides was revealed, of which the samples from Sichuan and Fujian were 0.81–1.30 mg·g−1 followed a descending order of vernine > cytidine > uridine > adenine > adenosine, and from Guangxi were 0.35–0.50 mg·g−1 with the sequences of uridine > adenine > vernine > cytidine > adenosine. The nucleosides content of the samples from Sichuan and Fujian was both higher than that from Guangxi. For the samples from Sichuan and Fujian, the former was slightly higher, except for adenine. These results would be helpful to reveal the bioactive constituents in aqueous extract and provided important evidences for the quality control of Rhizoma Alismatis.

【Keywords】 Rhizoma Alismatis; nucleosides; fingerprint; content determination;


【Funds】 National Chinese Medicine Standardization Project (ZYBZH-C-JL-24) Research Project on Standard Formulation and Revision of the Chinese Pharmacopoeia Commission (2018Z079)

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This Article


CN: 11-2272/R

Vol 45, No. 07, Pages 1558-1565

April 2020


Article Outline


  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References