Effect of processing on toxic components lectin from four kinds of Araceae toxic medicines

YU Hong-li1,2,3 WANG Wei1 WU Hao1,2,3 SHEN Min4 ZHANG Yuan-bin1 LI Shu-hui1

(1.Pharmacology College, Nanjing University of Chinese Medicine, Nanjing, China 210023)
(2.Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, China 210023)
(3.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing, China 210023)
(4.Suzhou Vocational Health College, Suzhou, China 215009)

【Abstract】The study aimed to investigate the effect of processing on lectin protein in four toxic Chinese medicines tubers of Pinellia ternata, P. pedatisecta, Arisema heterophyllum and Typhonium giganteum. Western blot was used to semi-quantitatively analyze the contents of lectins in the four kinds of toxic Chinese medicines and their different processed products. Raw products and lectin were treated by heating or soaking in ginger juice or alum solution. The effects of different excipients and the heating methods on lectin proteins were investigated. The results showed that the contents of lectins in the raw products of P. pedatisecta, P. ternata, A. heterophyllum, and T. giganteum were 7.3%, 4.9%, 2.7%, 2.3%, respectively. The content of lectin in Pinelliae Rhizoma Praeparatum cum Alumine was 0. 027%. Lectin was not detected in the Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine, Arisaematis Rhizma Praeparatum and Typhonii Rhizoma Praeparatum, which indicated that processing could significantly reduce the contents of active lectins in raw products. The results also showed that with the prolongation of soaking and heating time, the contents of lectins in raw products decreased gradually, while the content was almost unchanged when soaked in ginger juice alone. The effects of different excipients and heating on lectin were the same as those on raw products. Therefore, the method with alum soaking and heating can reduce the contents of active lectins, which is the key to reduce the toxicity of toxic Chinese medicines. In this paper, Western blot was used to study the content of toxic proteins in Araceae toxic Chinese medicines as an evaluation method of the processing degree.

【Keywords】 Araceae; toxic component; processing; lectin;

【DOI】

【Funds】 National Key Research and Development Program (2018YFC1707003) National Natural Science Foundation of China (81173549, 81573605)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 44, No. 24, Pages 5398-5404

December 2019

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References