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王涛1,2 张慧3 赵丹1,2 陈杰1,2 谢卓霖1,2 戴维1,2 叶坤浩1,2 黄晶4 陈岗福5,6

(1.绵阳市农业科学研究院中药材研究所, 四川绵阳 621023)
(2.国家中药材产业技术体系绵阳综合试验站, 四川绵阳 621023)
(3.绵阳市农业科学研究院生物技术中心, 四川绵阳 621023)
(4.西南科技大学生命科学与工程学院, 四川绵阳 621010)
(5.绵阳大地麦冬产业技术研究院, 四川绵阳 621023)
(6.四川代代为本农业科技有限公司, 四川绵阳 621100)

【摘要】对丹参波长叠加指纹图谱和7种丹参指标成分同时定量进行研究。采用Agilent Eclipse Plus C18 (4.6 mm×100 mm, 3.5μm) 色谱柱, 以0.1%甲酸的水溶液 (A) -乙腈 (B) 为流动相梯度洗脱 (05 min, 10%20%B;520 min, 20%30%B;2025 min, 30%50%B;2540 min, 50%65%B;4045 min, 65%80%B;4546 min, 80%10%B;4650 min, 10%B) , 流速1m L·min-1, 柱温30℃, 检测波长250, 280, 310, 340 nm。运用波长叠加技术建立指纹图谱, 并选取主要成分建立多指标测定方法。波长叠加指纹图谱体现了丹参250, 280, 310, 340 nm特征吸收波长的指纹信息, 17个不同来源的丹参样品与指纹图谱之间具有良好的相似性, 相似度在0.8280.936。确定了15个共有峰, 并指认了其中7个色谱峰, 分别是迷迭香酸、丹酚酸B、丹酚酸A、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA, 其方法学考察结果良好。建立了丹参波长叠加谱指纹图谱, 该方法简便可靠、耐用性好, 可用于丹参的质量控制。

【关键词】 丹参;高效液相色谱;波长叠加;指纹图谱;多指标成分;


【基金资助】 现代农业产业技术体系建设专项 (CARS-21) ;

Study on wavelength overlapping HPLC fingerprint and multi-component content determination of Radix Salviae Miltiorrhizae

WANG Tao1,2 ZHANG Hui3 ZHAO Dan1,2 CHEN Jie1,2 XIE Zhuo-lin1,2 DAI Wei1,2 YE Kun-hao1,2 HUANG Jing4 CHEN Gang-fu5,6

(1.Institute of Chinese Medicinal Materials, Mianyang Academy of Agricultural Sciences, Mianyang, Sichuan Province, China 621023)
(2.Comprehensive Experimental Station of Mianyang, National Industrial Technology System of Chinese Medicinal Materials, Mianyang, Sichuan Province, China 621023)
(3.Biotechnology Center, Mianyang Academy of Agricultural Sciences, Mianyang, Sichuan Province, China 621023)
(4.School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan Province, China 621010)
(5.Mianyang Industrial Technology Research Institute of Radix Ophiopogonis, Mianyang, Sichuan Province, China 621023)
(6.Sichuan Daidaiweiben Agricultural Science & Technology Co. Ltd., Mianyang, Sichuan Province, China 621100)

【Abstract】This project was launched to establish the wavelength overlapping HPLC fingerprint for Radix Salviae Miltiorrhizae and determine the contents of seven components in Radix Salviae Miltiorrhizae. The chromatographic fingerprints were built by using Agilent Eclipse Plus C18 (4.6 mm × 100 mm, 3.5 μm) as stationary phase and 0.1% formic acid solution (A) -acetonitrile (B) as mobile phase with gradient elution being (0–5 min, 10%–20% B; 5–20 min, 20%–30% B; 20–25 min, 30%–50% B; 25–40 min, 50%–65% B; 40–45 min, 65%–80% B; 45–46 min, 80%–10% B; 46–50 min, 10% B) at a flow rate of 1 m L·min–1. The column temperature was maintained at 30 °C and the detection wavelength was set at 250, 280, 310 and 340 nm. The technique of wavelength overlapping fingerprint was established and the contents of seven indicative compounds were determined. The results of wavelength overlapping HPLC fingerprint showed all-around information of the fingerprints at 250, 280, 310 and 340 nm, and the similarity among 17 batches of Radix Salviae Miltiorrhizae was within the range of 0.828–0.936. In wavelength overlapping HPLC fingerprint, 15 common peaks were selected as the common peaks, and seven contents of them were identified as rosmarinic acid, salvianolic acid B, salvianolic acid A, phenolic acid, dihydrotanshinone Ⅰ, tanshinone Ⅰ and tanshinone ⅡA. The results of methodological study demonstrated that the method met the requirements of the determination. The method established in this study is simple, convenient and durable, which can provide a scientific basis for the quality control of Radix Salviae Miltiorrhizae.

【Keywords】 Radix Salviae Miltiorrhizae; HPLC; wavelength overlapping; fingerprint; multi-component content;


【Funds】 Special Project for Construction of Modern Agricultural Industry Technology System (CARS-21);

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This Article


CN: 11-2272/R

Vol 44, No. 02, Pages 338-343

January 2019


Article Outline


  • 1 Materials
  • 2 Methods and results
  • 3 Discussion
  • References