Study on wavelength overlapping HPLC fingerprint and multi-component content determination of Radix Salviae Miltiorrhizae
(2.Comprehensive Experimental Station of Mianyang, National Industrial Technology System of Chinese Medicinal Materials, Mianyang, Sichuan Province, China 621023)
(3.Biotechnology Center, Mianyang Academy of Agricultural Sciences, Mianyang, Sichuan Province, China 621023)
(4.School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan Province, China 621010)
(5.Mianyang Industrial Technology Research Institute of Radix Ophiopogonis, Mianyang, Sichuan Province, China 621023)
(6.Sichuan Daidaiweiben Agricultural Science & Technology Co. Ltd., Mianyang, Sichuan Province, China 621100)
【Abstract】This project was launched to establish the wavelength overlapping HPLC fingerprint for Radix Salviae Miltiorrhizae and determine the contents of seven components in Radix Salviae Miltiorrhizae. The chromatographic fingerprints were built by using Agilent Eclipse Plus C18 (4.6 mm × 100 mm, 3.5 μm) as stationary phase and 0.1% formic acid solution (A) -acetonitrile (B) as mobile phase with gradient elution being (0–5 min, 10%–20% B; 5–20 min, 20%–30% B; 20–25 min, 30%–50% B; 25–40 min, 50%–65% B; 40–45 min, 65%–80% B; 45–46 min, 80%–10% B; 46–50 min, 10% B) at a flow rate of 1 m L·min–1. The column temperature was maintained at 30 °C and the detection wavelength was set at 250, 280, 310 and 340 nm. The technique of wavelength overlapping fingerprint was established and the contents of seven indicative compounds were determined. The results of wavelength overlapping HPLC fingerprint showed all-around information of the fingerprints at 250, 280, 310 and 340 nm, and the similarity among 17 batches of Radix Salviae Miltiorrhizae was within the range of 0.828–0.936. In wavelength overlapping HPLC fingerprint, 15 common peaks were selected as the common peaks, and seven contents of them were identified as rosmarinic acid, salvianolic acid B, salvianolic acid A, phenolic acid, dihydrotanshinone Ⅰ, tanshinone Ⅰ and tanshinone ⅡA. The results of methodological study demonstrated that the method met the requirements of the determination. The method established in this study is simple, convenient and durable, which can provide a scientific basis for the quality control of Radix Salviae Miltiorrhizae.
【Keywords】 Radix Salviae Miltiorrhizae; HPLC; wavelength overlapping; fingerprint; multi-component content;
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