Chemical constituents from root barks of Dictamnus dasycarpus and its cytotoxic activities

GUO Xi-xi1 ZHAO Li-na1 WANG Jia1 LIU Shuai2 BI Qi-rui1 WANG Zhe1 TAN Ning-hua1

(1.School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu Province, China 211198)
(2.Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu Province, China 223001)

【Abstract】Nineteen compounds, including kihadanin D (1), obacunone (2), kihadanin A (3), kihadanin B (4), kihadanin C (5), limonin (6), evodol (7), fraxinellone (8), furo[2,3-b]quinolin-4-ol (9), preskimmianine (10), ifflaiamine (11), dictamnol (12), naringenin (13), diosmetin (14), wogonin (15), scopoletin (16), cleomiscosin A (17), apocynin (18), and methyl pyroglutamate (19), were isolated from the methanol extract of the root barks of Dictamnus dasycarpus by using various column chromatographies. Their chemical structures were extensively determined on basis of UV, IR, NMR, MS, and CD spectroscopic data analyses. Among them, compound 1 was a new limonoid, compound 9 was isolated from plant kingdom for the first time, and compounds 11, 1314 and 1719 were obtained from the genus Dictamnnus for the first time. Cytotoxicities of compounds 118 were tested, and the results indicated that 1 exhibited cytotoxicities against three human cancer cell lines MDA-MB-231, A549, and HT29 with IC50 values of 16.22, 21.72, and 31.06 μmol·L−1, respectively.

【Keywords】 Dictamnus dasycarpus; chemical constituents; limonoids; kihadanin D; cytotoxicity;

【DOI】

【Funds】 Natural Science Foundation of China (31470428 and 21702231) Foundation of “Double Creative Team” in Jiangsu Province Fundamental Research Funds for the Central Universities of Ministry of Education of China (2632017PY19)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 43, No. 24, Pages 4869-4877

December 2018

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Article Outline

Abstract

  • 1 Materials
  • 2 Extraction and isolation
  • 3 Structural identification
  • 4 Cytotoxic activity (MTS method)
  • 5 Discussion
  • References