Extraction kinetics of volatile oil from galangal by steam distillation
【Abstract】To reveal the extraction regularity of volatile oil from galangal by GC-MS analysis. The volatile oil in galangal was extracted by steam distillation. The extract was collected every 30 min, the oil part and the water part were separated. GC-MS was used to analyze the extraction liquid collected at different time periods. A total of 140 volatile components were obtained by GC-MS analysis. Among them, the main components were eucalyptus oil alcohol, alpha-pine oil alcohol and 4-terpene alcohol; 22 special components were dissolved in water, 77 special components were dissolved in oil and 41 components were dissolved in both oil and water. With the increase of specific components in water, the content of Eucalyptus in water increased in a linear manner. The increase of eucalyptus oil further promoted the dissolution or dispersion of alpha PN in water, and the change of specific components in oil was positively correlated with the content of Eucalyptus and alpha-terpilenol in oil. The results of principal component analysis show that the physical and chemical properties of the compounds were important factors affecting the distribution of components. PC1 (molecular weight, melting point, boiling point positive correlation), PC2 (negative correlation of refractive index) and PC3 (positive correlation of water solubility) were the main components that lead to the differences in composition distribution. The process of extracting volatile oil from galangal through steam distillation was affected by the physical and chemical properties of volatile components. Some components were specifically distributed in the fragrance and volatile oil system. The endemic components of aromatic water increased the content of the main components in the water system, which may lead to the “emulsification”, reduction of the yield and low quality of the volatile oil.
【Keywords】 galangal; volatile components; GC-MS; double internal standard method; extraction kinetics;
 Zhang Q H, Wang Z P. Progress in Extractive Technique of Volatile Oil from Traditional Chinese Medicine [J]. Food and Drug, 2009, 11 (3): 62 (in Chinese).
 Rong R, Qiu L L, Zhang Y P, et al. Gc-msanalysis for volatile compounds in rhizoma alpiniae officinarum prepared by steam distillation or headspace injection [J]. Chemical Analysis and Meterage, 2010, 19 (4): 41 (in Chinese).
 Chinese Pharmacopeia. Section 1. [S]. 2015 (in Chinese).
 Dong D D, Cai B C. Studies on chemical constituents of the volatile oil of Alpinia officinarum Hance by GC-MS [J]. China Modern Medicine, 2015, 34 (22): 9 (in Chinese).
 Tan Y F, Li H L, Li Y B, et al. Identification of Chemical Constituents Occurring in Leaves of Alpinia officinarum [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2015, 21 (3): 37 (in Chinese).
 Mo D D, Liu J H, Qiu H B, et al. Materiamedica literature study on medicinal properties and utility of four kinds of Alpinia Chinese medicines [J]. China Journal of Chinese Materia Medica, 2018, 43 (13): 2648 (in Chinese).
 Zhang X G, Yin H, Chen F, et al. Advances in Study of Pharmacological Activities of Galangin [J]. Modern Chinese Medicine, 2016, 18 (11): 1532 (in Chinese).
 Xiong Y G, Shen Y, Zhang H. Advances in pharmacological activity of Alpinia Officinaruim Hance [J]. Central South Pharmacy, 2017, 15 (10): 1418 (in Chinese).
 Li H F, Li Y H, Wang Y, et al. Advances in Studies on Chemical Constituents in Alpiniae Officinarum Rhizoma and their Pharmacological Activities [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2014, 20 (7): 236 (in Chinese).
 Fan S L, Liu L P, Bian K J. Determination of Five Active Constituents of Asiasarum Essential Oil with Capillary Gas Chromatography by Using Double-Internal Standard Method [J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27 (10): 909 (in Chinese).
 Yang Y Q, Chu G H, Zhou G J, et al. GC-MS Determination of Aromatizing Constituents from Pyrolysis Tobacco with Dual-Internal Standards [J]. Physical Testing and Chemical Analysis(Part B:Chemical Analysis), 2016, 11 (52): 1272 (in Chinese).
 Chen L X, Zhang W X, Li S P, et al. Analysis of volatile components in fresh/dried Alpinia officinarum by HS-SPME-GC-MS* [J]. Chinese Journal of Pharmaceutical Analysis, 2017, 9 (37): 1615 (in Chinese).
 Wang H, Zhai H L, Mei W L, et al. GC-MS Analysis of Volatile Oil Extracted from Alpinia Officinarum Growing in Hainan [J]. Journal of Tropical Biology, 2014, 5 (2): 182 (in Chinese).
 Zhai H L, Wang H, Zeng Y B, et al. GC-MS Analysis of Volatile Oil from Alpinia officinarum Hance in Two Different Producing Areas [J]. Chinese Journal of Tropical Crops, 2013, 34 (12): 2475 (in Chinese).
 Teng H Y, Qu L P, Li X L, et al. 气相色谱-质谱结合化学计量学方法分析中药挥发油成分的研究进展 [J]. Journal of Pharmaceutical Practice, 2010, 28 (4): 245 (in Chinese).
 Lai Z P, Cheng X X, Yang Q, et al. Composition Analysis on 1, 8-Cineole and Volatile Oil in Alpinia officinarum Hance from Different Regions [J]. Asia-Pacific Traditional Medicine, 2010, 6 (9): 6 (in Chinese).
 Hui Y Z. Comprehensive Natural Products in Chinese Materia Medica [M]. Shanghai city: Shanghai Scientific & Technical Publishers, 2011:1492 (in Chinese).
 Li X, Xie S D, Lv L, et al. 中药挥发油提取中存在的问题及解决办法 [J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2006 (3): 179 (in Chinese).
 Huang L S, Gu Y F, Li H. Advances in herbalvolatile oil and aromaticherbs [J]. China Journal of Chinese Materia Medica, 2009, 34 (12): 1605 (in Chinese).