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甘草炮制雷公藤降低其肝毒性作用的初步研究

赵小梅1 宫嫚2 董捷鸣1 王伽伯3 肖小河2 赵奎君1 马致洁1,3

(1.首都医科大学附属北京友谊医院, 北京 100050)
(2.中国人民解放军第三O二医院中西医结合肝病诊疗与研究中心, 北京 100039)
(3.中国人民解放军第三O二医院全军中医药研究所, 北京 100039)

【摘要】为探讨甘草炮制雷公藤降低其肝毒性的作用,动物实验评价炮制前后雷公藤对肝脏的毒性,观察空白组、雷公藤组、甘草炮制雷公藤组肝组织病理切片、血清生化指标及细胞炎症因子的差异。大鼠肝组织病理切片显示雷公藤组肝组织损伤明显,炮制后肝组织未见明显损伤;雷公藤组与空白组比较AST,ALT,CRE升高(P<0.01),UREA升高(P<0.05),ALB降低(P<0.01);甘草炮制雷公藤组与雷公藤组比较AST,ALT,CRE,UREA降低(P<0.01),ALB升高(P<0.01)。炎症因子检测结果显示雷公藤组与空白组比较IL-1β,IL-6,TNF-α显著升高(P<0.01);甘草炮制雷公藤组与雷公藤组比较IL-1β,IL-6,TNF-α显著降低(P<0.01)。甘草炮制雷公藤可有效降低雷公藤肝毒性,减轻雷公藤导致的肝损伤。该实验可为雷公藤合理制用提供参考,同时为甘草炮制雷公藤降低其肝毒性研究提供数据支持。

【关键词】 雷公藤;甘草;炮制减毒;肝毒性;炎症因子;

【DOI】

【基金资助】 北京市中医管理局项目(QN2014-18); 首都中医药研究专项(15ZY04); 北京友谊医院院启动项目(YYQDKT2014-20); 北京市医院管理局“青苗”人才培养计划项目(QML20160106); 首都卫生发展科研专项项目(首发2016-4-2024);

Preliminary research on effect of licorice-processed Tripterygium wilfordii on reducing liver toxicity

ZHAO Xiao-mei1 GONG Man2 DONG Jie-ming1 WANG Jia-bo3 XIAO Xiao-he2 ZHAO Kui-jun1 MA Zhi-jie1,3

(1.Beijing Friendship Hospital, Capital Medical University, Beijing, China 100050)
(2.Integrative Medicine Center for Liver Disease Diagnosis and Treatment, 302 Military Hospital of China, Beijing, China 100039)
(3.China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing, China 100039)

【Abstract】To explore the effect of the licorice-processed Tripterygium wilfordii on reducing the liver toxicity. In animal experiments, the liver toxicity of T. wilfordii was evaluated both before and after processing, and the differences in liver tissue biopsy, serum biochemical indexes and inflammatory cell factor among blank group, T. wilfordii group and licorice-processed T. wilfordii group were observed. Liver tissue biopsy results showed that liver tissue injury was obvious in T. wilfordii group, and no obvious injury was found in licorice-processed T. wilfordii group. As compared with the blank group, the levels of AST, ALT and CRE were significantly increased (P < 0.01), UREA was increased (P < 0.05), and ALB level was significantly decreased (P < 0.01) in the T. wilfordii group. As compared with T. wilfordii group, the levels of AST, ALT, CRE, and UREA were decreased (P < 0.01), while ALB was increased (P < 0.01) in the licorice-processed T. wilfordii group. The results of inflammatory factors in rats showed that the levels of IL-1β, IL-6, and TNF-α in T. wilfordii group were significantly higher than those in blank group (P < 0.01); the levels of IL-1β, IL-6, and TNF-α in licorice-processed T. wilfordii group were significantly lower than those in T. wilfordii group (P < 0.01). Overall, licorice processing of T. wilfordii can effectively reduce the liver toxicity and reduce the liver injury caused by T. wilfordii. The experiment can provide reference for the clinical rational use of the T. wilfordii, and provide data support for the studies on reducing the liver toxicity of T. wilfordii by licorice processing.

【Keywords】 Tripterygium wilfordii; licorice; processing attenuated; liver toxicity; inflammatory factor;

【DOI】

【Funds】 Fund Project of Beijing Municipal Administration of Traditional Chinese Medicine (QN2014-18); Special Project of Beijing University of Chinese Medicines (15ZY04); Initiation Project of Beijing Friendship Hospital (YYQDKT2014-20); “Young Crop” Talents Training Plan Project of Beijing Municipal Administration of Hospitals (QML20160106); Program of the Capital Health Research and Development (2016-4-2024);

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 42, No. 01, Pages 119-124

January 2017

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Article Outline

Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Conclusions
  • References