(2.中国人民解放军第三O二医院中西医结合肝病诊疗与研究中心, 北京 100039)
(3.中国人民解放军第三O二医院全军中医药研究所, 北京 100039)
【基金资助】 北京市中医管理局项目(QN2014-18); 首都中医药研究专项(15ZY04); 北京友谊医院院启动项目(YYQDKT2014-20); 北京市医院管理局“青苗”人才培养计划项目(QML20160106); 首都卫生发展科研专项项目(首发2016-4-2024);
Preliminary research on effect of licorice-processed Tripterygium wilfordii on reducing liver toxicity
(2.Integrative Medicine Center for Liver Disease Diagnosis and Treatment, 302 Military Hospital of China, Beijing, China 100039)
(3.China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing, China 100039)
【Abstract】To explore the effect of the licorice-processed Tripterygium wilfordii on reducing the liver toxicity. In animal experiments, the liver toxicity of T. wilfordii was evaluated both before and after processing, and the differences in liver tissue biopsy, serum biochemical indexes and inflammatory cell factor among blank group, T. wilfordii group and licorice-processed T. wilfordii group were observed. Liver tissue biopsy results showed that liver tissue injury was obvious in T. wilfordii group, and no obvious injury was found in licorice-processed T. wilfordii group. As compared with the blank group, the levels of AST, ALT and CRE were significantly increased (P < 0.01), UREA was increased (P < 0.05), and ALB level was significantly decreased (P < 0.01) in the T. wilfordii group. As compared with T. wilfordii group, the levels of AST, ALT, CRE, and UREA were decreased (P < 0.01), while ALB was increased (P < 0.01) in the licorice-processed T. wilfordii group. The results of inflammatory factors in rats showed that the levels of IL-1β, IL-6, and TNF-α in T. wilfordii group were significantly higher than those in blank group (P < 0.01); the levels of IL-1β, IL-6, and TNF-α in licorice-processed T. wilfordii group were significantly lower than those in T. wilfordii group (P < 0.01). Overall, licorice processing of T. wilfordii can effectively reduce the liver toxicity and reduce the liver injury caused by T. wilfordii. The experiment can provide reference for the clinical rational use of the T. wilfordii, and provide data support for the studies on reducing the liver toxicity of T. wilfordii by licorice processing.
【Keywords】 Tripterygium wilfordii; licorice; processing attenuated; liver toxicity; inflammatory factor;
【Funds】 Fund Project of Beijing Municipal Administration of Traditional Chinese Medicine (QN2014-18); Special Project of Beijing University of Chinese Medicines (15ZY04); Initiation Project of Beijing Friendship Hospital (YYQDKT2014-20); “Young Crop” Talents Training Plan Project of Beijing Municipal Administration of Hospitals (QML20160106); Program of the Capital Health Research and Development (2016-4-2024);
Xie Yan, Guo Yunpeng. Research progress on the mechanism of Tripterygium wilfordii in treating rheumatoid arthritis [J]. Rheunatism and Arthritis, 2013(4): 72 (in Chinese).
Liu Shizhong. Clinical observation of Compound Ammonium Glycyrrhetate Combined with Tripterygium Wilfordii Polyglycoside Tablets in Treating Psoriasis Vulgaris [J]. Chinese Journal of Modern Drug Application, 2014, 8(1) : 138 (in Chinese).
Center for Drug Reevaluation, CFDA. Concerning Medication Safety of Tripterygium Wilfordii Preparations [J]. Chinese Adverse Drug Reaction Information Bulletin [J], 2012, 46 (4) : 1 (in Chinese).
Teng Guangju, Liang Qingsheng, Sun Ying, et al. Clinical Features and Pathological Analysis of 186 Patients with Liver Injury Induced by Chinese Herbal Medicine[J]. Chinese Archives of Traditional Chinese Medicine, 2014, 32(4): 913–916 (in Chinese).
Liu Jianqun, Gao Junbo, Shu Jicheng, et al. Effects of Microwave Processing on Tripterygium Wilfordii Toxicity and its Chemical Components [J]. Lishizhen Medicine and Materia Medica Research, 2014(2): 344 (in Chinese).
Xie Suhua, Zhang Guangping, Sun Guibo, et al. Detoxication experimental study on different compatibility proportion of Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma[J]. China Journal of Chinese Materia Medica, 2012, 37(15): 2210–2214 (in Chinese).
Li Zhihua, Yan Miao, Zhang Bikui, et al. Advances in study on compatibility of licorice and its mechanism of detoxification based on pharmacokinetics[J]. Chinese Traditional and Herbal Drugs, 2015, 46(23): 3611–3616 (in Chinese).
Chen Yunhua, Wan Xin, Sun Jianning, et al. Hepato-protective Activity of Glycyrrhizin, Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(4): 245–248 (in Chinese).
Ma Zhe. Reducing Toxicity and Increasing Effect of Tripterygium Wilfordii combined with Liquorice [D]. Shenyang: Liaoning University of Traditional Chinese Medicine, 2001 (in Chinese).
Zhou Cong, Zhou Lingling, Zhou Xueping, et al. Study on the mechanism of attenuating hepatotoxicity of Tripterygium wilfordii through compatibility[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2014, 29(4): 1167–1169 (in Chinese).
Cao Lingjuan, Yan Miao, Li Huande, et al. Progress on mechanism of Tripterygium wilfordii-induced liver injury and detoxification mechanism of licorice[J]. China Journal of Chinese Materia Medica, 2015, 40(13): 2537–2541 (in Chinese).
Xie Tong, Zhou Xueping, Lin Lili, et al. Metabolomics analysis of Tripterygium wilfordii formulation based on theory of detoxicity compatibility[J]. China Journal of Chinese Materia Medica, 2016, 41(6): 1124–1129 (in Chinese).
Chai Zhi, Fan Huijie, Zhou Wenjing, et al. Effects of Xiaoyao Powder on CD68 and TNF-α in Rats with Tripterygium-Induced Liver Injury[J]. Journal of Traditional Chinese Medicine, 2014, 55(6): 497–499 (in Chinese).
Wang Xuefu, Sun Rui, Wei Haiming, et al. High–mobility group box1 (HMGB1)-toll-like receptor (TLR) 4-interleukin (IL)-23-IL-17A axis in drug-induced damage-associated lethal hepatitis: interaction of γδT cells with macrophages[J]. Hepatology, 2013, 57: 373.
Masatoshi Ohnishi, Hiroshi Katsuki, Chiharu Fukutomi, et al. HMGB1 inhibitor glycyrrhizin attenuates intracerebral hemorrhage–induced injury in rats[J]. Neuropharmacology, 2011, 61: 975.
Yan Ge, Yang Jiamei. Research Progress of High–mobility Group Protein B1 and Liver Damage. 肝胆外科杂志, 2010(5): 392 (in Chinese).
Wang X, Jiang Z, Cao W, et al. Th17/Treg imbalance in triptolide-induced liver injury[J]. Fitoterapia, 2014, 93: 245.
Xue X, Gong L, Qi X, et al. Knockout of hepatic P450 reductase aggravates triptolide-induced toxicity[J]. Toxicol Lett, 2011, 205(1): 47.
Yao Jincheng, Liu Ying, Hu Ling, et al. Study on the mechanism of triptolide for inducing apoptosis of human liver cells[J]. Chinese Journal of New Drugs, 2013, 22(6): 698–703 (in Chinese).
Zhang Y, Jiang Z, Xue M, et al. Toxicogenomic analysis of the gene expression changes in rat liver after a 28-day oral Tripterygium wilfordii multiglycoside exposure[J]. J Ethnopharmacol, 2012, 141(1): 170.
Fu Q, Huang X, Shu B, et al. Inhibition of mitochondrial respiratory chain is involved in triptolide-induced liver injury[J]. Fitoterapia, 2011, 82(8): 1241.