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Optimization of Concentration Process of Alkaloid from Leonurus japonicus by Ultrafiltration-nanofiltration Coupling Technology

LI Cun-yu1,2 MA Yun1 LIU Yi-zhou1 LI Hong-yang1 GU Jia-mei1 PENG Guo-ping1,2

(1.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China 210023)
(2.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, China 210023)

【Abstract】The purpose of the present study is to optimize the concentrate process of alkaloid from Leonurus japonicus by ultrafiltration-nanofiltration coupling technology together with response surface methodology. The experiment showed that after ultrafiltration pre-treatment, the total protein removal rate was 94.38% in aqueous extract from L. japonicus, and the nanofiltration technology had obvious advantages over the conventional concentrate process. The optimal concentrate conditions were as follows: molecular weight cut-off 450, pH 3.07, concentration of stachydrine hydrochloride 80.15 mg·L−1, and concentration of the total alkaloid 285.73 mg·L−1. The rejection was 93.37% and 95.85% respectively for stachydrine hydrochloride and the total alkaloid under the optimum conditions, with the relative error of 0.79% and 1.16% respectively. The combination of Box-Behnken design and response surface analysis can well optimize the concentrate process of L. japonicus by nanofiltration, and the results provide the basis for nanofiltration concentrate for heat-sensitive traditional Chinese medicine.

【Keywords】 alkaloid; Leonurus japonicas; stachydrine hydrochloride; nanofiltration; concentrate; ultrafiltration; alkaloid; Leonurus japonicas; stachydrine hydrochloride; nanofiltration; concentrate; ultrafiltration;


【Funds】 National Natural Science Foundation of China (81503258) Youth Fund Project of Jiangsu Province Natural Science Foundation (BK20151005) Jiangsu Provincial Bureau of Traditional Chinese Medicine Science and Technology Project (YB2015009)

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This Article



Vol 42, No. 01, Pages 100-106

January 2017


Article Outline


  • 1 Materials
  • 2 Methods
  • 3 Results and analysis
  • 4 Summary and conclusions
  • References