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李存玉1,2 马赟1 刘奕洲1 李红阳1 顾佳美1 彭国平1,2

(1.南京中医药大学药学院, 江苏南京 210023)
(2.江苏省中药资源产业化过程协同创新中心, 江苏南京 210023)

【摘要】采用超滤-纳滤联用技术浓缩益母草中生物碱,通过响应面法建立二次回归模型,优化浓缩工艺。实验表明通过超滤预处理,益母草水提液中总蛋白去除率94.38%,纳滤技术相较于传统热浓缩优势明显,益母草生物碱的最佳浓缩工艺为截留相对分子质量450,p H 3.07,盐酸水苏碱质量浓度80.15 mg·L-1,总生物碱质量浓度285.73 mg·L-1,在此条件下,盐酸水苏碱和总生物碱的截留率分别为93.37%,95.85%,相对误差为0.79%,1.16%,Box-Behnken试验设计方法可以用于益母草生物碱的浓缩工艺优化,纳滤浓缩为热敏性中药成分的分离精制提供技术支撑。

【关键词】 生物碱;益母草;盐酸水苏碱;纳滤;浓缩;超滤;


【基金资助】 国家自然科学基金项目(81503258); 江苏省自然科学基金青年基金项目(BK20151005); 江苏省中医药局科技项目(YB2015009);

Optimization of Concentration Process of Alkaloid from Leonurus japonicus by Ultrafiltration-nanofiltration Coupling Technology

LI Cun-yu1,2 MA Yun1 LIU Yi-zhou1 LI Hong-yang1 GU Jia-mei1 PENG Guo-ping1,2

(1.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China 210023)
(2.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, China 210023)

【Abstract】The purpose of the present study is to optimize the concentrate process of alkaloid from Leonurus japonicus by ultrafiltration-nanofiltration coupling technology together with response surface methodology. The experiment showed that after ultrafiltration pre-treatment, the total protein removal rate was 94.38% in aqueous extract from L. japonicus, and the nanofiltration technology had obvious advantages over the conventional concentrate process. The optimal concentrate conditions were as follows: molecular weight cut-off 450, pH 3.07, concentration of stachydrine hydrochloride 80.15 mg·L−1, and concentration of the total alkaloid 285.73 mg·L−1. The rejection was 93.37% and 95.85% respectively for stachydrine hydrochloride and the total alkaloid under the optimum conditions, with the relative error of 0.79% and 1.16% respectively. The combination of Box-Behnken design and response surface analysis can well optimize the concentrate process of L. japonicus by nanofiltration, and the results provide the basis for nanofiltration concentrate for heat-sensitive traditional Chinese medicine.

【Keywords】 alkaloid; Leonurus japonicas; stachydrine hydrochloride; nanofiltration; concentrate; ultrafiltration;


【Funds】 National Natural Science Foundation of China (81503258); Youth Fund Project of Jiangsu Province Natural Science Foundation (BK20151005); Jiangsu Provincial Bureau of Traditional Chinese Medicine Science and Technology Project (YB2015009);

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This Article



Vol 42, No. 01, Pages 100-106

January 2017


Article Outline


  • 1 Materials
  • 2 Methods
  • 3 Results and analysis
  • 4 Summary and conclusions
  • References