DNA Marker-assisted Selection of Medicinal Plants (Ⅰ). Breeding Research of disease-resistant cultivars of Panax notoginseng

DONG Lin-lin1 CHEN Zhong-jian2 WANG Yong2 WEI Fu-gang3 ZHANG Lian-juan1 XU Jiang1 WEI Guang-fei1 WANG Rui1 YANG Juan1 LIU Wei-lin3 LI Xi-wen1 YU Yu-qi3 CHEN Shi-lin1

(1.Institute of Chinese Materia Media, China Academy of Chinese Medicinal Sciences, Beijing, China 100700)
(2.Institute of Sanqi Research, Wenshan University, Wenshan, China 663000)
(3.Wenshan Miaoxaing Notoginseng Technology Co., Ltd., Wenshan, China 663000)

【Abstract】DNA marker-assisted selection of medicinal plants is based on the DNA polymorphism, selects the DNA sequences related to the phenotypes such as high yields, superior quality, stress resistance according to the technologies of molecular hybridization, polymerase chain reaction and high-throughput sequencing, and assists the breeding of new cultivars. This study bred the first disease-resistant cultivar of Panax notoginseng “Miaoxiang Kangqi 1” using the technology of DNA marker-assisted selection of medicinal plants and systematic breeding. The disease-resistant cultivar of P. notoginseng contained 12 special SNPs based on the analysis of Restriction-site Associated DNA Sequencing (RAD-Seq). The SNP (record_519688) was verified to be related to the root rot-resistant characteristics, which indicated this SNP could serve as genetic markers of disease-resistant cultivars and assist the systematic breeding. Compared to the conventional cultivated cultivars, the incidence rate of root rot and rust rot in P. notoginseng seedlings decreased by 83.6% and 71.8%, respectively. The incidence rate of root rot respectively declined by 43.6% and 62.9% in biennial and triennial P. notoginseng cultivation compared with those of the conventional cultivated cultivars. Additionally, the potential disease-resistant groups were screened based on the relative SNP, and this model enlarged the target groups and advanced the breeding efficiency. DNA marker-assisted selection of medicinal plants accelerated the breeding and promotion of new cultivars, and guaranteed the healthy development of Chinese medicinal materials industry.

【Keywords】 medicinal plants; DNA marker-assisted selection; Panax notoginseng; continuous cropping obstacles; disease-resistant cultivars; restriction-site associated DNA sequencing; single-nucleotide polymorphism;

【DOI】

【Funds】 National Natural Science Foundation of China (81603238, 81403053)

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This Article

ISSN:1001-5302

CN: 11-2272/R

Vol 42, No. 01, Pages 56-62

January 2017

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results
  • 4 Discussion
  • References