Research of reversal effect of PESV on multi-drug resistance of leukemia stem cell

YANG Xiang-dong1 WANG Xing-li1 YANG Wen-hua1 ZHAO Lei1 YAN Tian-gai1

(1.First Teaching Hospital of Tianjin University of TCM, Tianjin, China 300381)

【Abstract】The BALB/c mouse models with multidrug-resistant (MDR) leukemia were applied in the study to observe the effects of peptide extract from scorpion venom (PESV) on the upstream signal factors of P-gp in the leukemia stem cell (LSC), and to study the mechanism of PESV in reversing the MDR of LSC. At the same time, the expression levels of P-gp, MDR1 mRNA and PI3-K, NF-κB were respectively detected by flow cytometry, RT-PCR, Western blot and Elisa, and the histopathological methods were used to examine the mouse liver and spleen. The results of the experiment were as follows: mice of the control group didn’t show any obvious change, while those in the other six groups all showed such symptoms as arched back and emaciation. The liver was enlarged, and inflammation and necrosis were found in all liver tissue. The expression levels of P-gp and PI3 K on the LSC membrane of mouse tumor were down-regulated; The expression level of MDR1 mRNA in the cytoplasm was obviously down-regulated in the low-dose PESV group, while they were up-regulated in the middle-dose group and the high-dose group to different degrees. The expression level of NF-κB in the cell nucleus of LSC was remarkably decreased. PESV played an outstanding role in down-regulating PI3K/NF-κB/MDR1 at the upstream region of P-gp, and it could enhance the sensitivity of LSCs to ADM to a certain degree. Therefore, this experiment explained one of the possible mechanisms of PESV in reversing MDR of LSCs, thus providing the foundation for further study on combinational anti-cancer effects of PESV.

【Keywords】 peptide extract from scorpion venom (PESV) ; multi-drug resistance (MDR); BALB/c mouse model; upstream signal factors of P-gp;


【Funds】 Project Supported by Science and Technology Commission of Tianjin Municipality (12JCQNJC09000)

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(Translated by WU XX)


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This Article


CN: 11-2272/R

Vol 41, No. 24, Pages 4648-4653

December 2016


Article Outline


  • 1 Materials
  • 2 Methods
  • 3 Results
  • 4 Discussions
  • References