Germplasm identification of Lonicerae Japonicae Flos by DNA melting curve analysis

CHEN Ti-ying1 JIANG Chao1 YUAN Yuan1 CHEN Kang1 ZHOU Jun-hui1 ZHAO Yu-yang1 HUANG Lu-qi1

(1.State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China 100700)

【Abstract】To establish a new high resolution melting analytical method for identification of Lonicera japonica germplasm, the screening of 7 pairs of simple sequence repeats (SSRs) primers, determining the suitable diagnostic primers by the differences of peak pattern and Tm were conducted. Then into the DNA template concentration, annealing temperature and the suitable range of cycle number were investigated. Combined with SIMCA-P software for data processing analysis, the results showed that three main germplasm honeysuckle could be divided by four sets of primers. It provided methodology for improving L. japonica germplasm identification.

【Keywords】 high resolution melting curve; Lonicerae Japonicae Flos; germplasm identification;


【Funds】 National Natural Science Foundation of China (81373959) Special Project of the Ministry of Scienceand Technology (2015FY111500) Special Fund for Scientific Research of Traditional Chinese Medicine Scientific Research (201407003)

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This Article


CN: 11-2272/R

Vol 41, No. 24, Pages 4572-4578

December 2016


Article Outline


  • 1 Materials
  • 2 Methods
  • 3.1 Results and analyses
  • 4 Discussion
  • References