Astragaloside IV Regulates STAT1/Iκ/NF-κB Signaling Pathway to Inhibit Activation of BV-2 Cells

HE Yi-xin1,2 SHI Hai-lian LIU Hong-shuai WU Hui ZHANG Bei-bei WU Xiao-jun WANG Zheng-tao1,2

(1.Department of Pharmacognosy, School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, China 210009)
(2.Shanghai Key Laboratory of Complex Prescriptions, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai,China 201203)

【Abstract】Objective: The study was aimed to investigate the inhibitory effect and mechanism of astragaloside IV( ASI) on the activation of microglial cells. Method: After pre-incubated with ASI for 2 h,microglial cells BV-2 were stimulated with interferon-γ( IFN-γ) for 1. 5 h and 24 h,respectively. Secretion of nitric oxide( NO) in the medium was measured by Griess method. Production of tumor necrosis factor alpha( TNF-α) was detected by ELISA approach. Cellular gene expressions of CD11 b,TNF-α,interleukin 1β( IL-1β) and induced nitric oxide synthase( i NOS) were examined by quantitative-PCR analysis. Total and phosphorylation of STAT1,IκB and NF-κB was analyzed by Western blot method. Result: ASI could significantly inhibit the increased secretion of TNF-α and NO from BV-2 cells upon IFN-γ stimulation( P < 0. 001). Further study showed that ASI significantly down-regulated gene expression of IL-1β and TNF-α( P < 0. 01,P < 0. 05) and exhibited a trend to reduce that of i NOS. IFN-γ and ASI have no obvious effect on gene expression of CD11 b. Moreover,ASI inhibited the phosphorylation of STAT1,IκB and NF-κB elicited by IFN-γ stimulation.Conclusion: ASI could restrain microglial activation through interfering STAT1 / IκB / NF-κB signaling pathway,reducing gene expression of IL-1β and TNF-α,and thus inhibiting the production of proinflammatory mediators such as NO and TNF-α.

【Keywords】 astragaloside IV; microglia cells; neuroinflammation; STAT1; IκB; NF-κB;

【DOI】

【Funds】 the Foundation for Priority Area of the Ministry of Education of China for doctors in University (Grant No. 20123107130002) Key Innovative Program of Board of Education in Shanghai (Grant No. 13ZZ099) Program for Special Term Professor (Orientalist) in Shanghai universities(Grant No. 2013-59)

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This Article

ISSN:0255-2930

CN: 11-2024/R

Vol 35, No. 02, Pages 132-136

February 2015

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Abstract

  • 1 Materials
  • 2 Methods
  • 3. Results
  • 4 Discussion
  • References