Effect of electroacupuncture at governor vessel on learning-memory ability and serum levels of APP and Aβ1-42 in patients with Alzheimer’s disease

XIA Kun-peng1,2 PANG Jing1,2 LI Shu-lin2 ZHANG Miao2 LI Hong-lin2 WANG Yu-jue2

(1.Graduate School of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China 150040)
(2.Third Ward of Rehabilitation Department, Second Clinical Medical College of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China 150001)

【Abstract】 Objective To compare the therapeutic effect of electroacupuncture (EA) combined with donepezil hydrochloride with that of donepezil hydrochloride alone in improving learning-memory ability in patients with Alzheimer’s disease (AD), and to explore its action mechanism. Methods Sixty patients of AD were randomly divided into an observation group and a control group, with 30 cases in each group. The patients in the observation group were treated with EA at governor vessel combined with donepezil hydrochloride. EA was applied at Baihui (GV20) and Fengfu (GV16), and the dilatational wave was selected for bearable stimulation at the frequency of 10 Hz/50 Hz and the intensity of 0.5–5.0 mA. The needles were retained for 40 min. EA was given once a day. The donepezil hydrochloride tablet was taken orally, 5 mg per time, once a day, and after four weeks of treatment, the dosage might be increased to 10 mg per day according to the specific situation. All the treatments lasted for eight weeks. The patients in the control group were only treated with donepezil hydrochloride in the same way as that in the observation group. The Montreal Cognitive Assessment (MoCA) and Alzheimer’s disease Assessment Scale-cognitive Subscale (ADAS-Cog) were evaluated before and after treatment; P300 (latencies and amplitudes of N2 and P3) was detected by the electroencephalograph (EEG) / event-related brain potential (ERP) system, and amyloid precursor protein (APP) and β-amyloid protein 1-42 (Aβ1-42) were detected by ELISA. Results Compared with those before treatment, the MoCA scores were increased after treatment in the two groups (P < 0.05), and the MoCA score in the observation group was higher than that in the control group (P < 0.05). Compared with those before treatment, the ADAS-Cog scores were decreased after treatment in the two groups (P < 0.05), and the ADAS-Cog score in the observation group was lower than that in the control group (P < 0.05). Compared with those before treatment, the latencies of N2 and P3 were shortened and the amplitudes were increased after treatment in the two groups (P < 0.05). After treatment, the latencies of N2 and P3 in the observation group were shorter than those in the control group and the amplitudes were higher than those in the control group (P < 0.05). Compared with those before treatment, the serum levels of APP and Aβ1-42 were decreased after treatment in the two groups (P < 0.05), and the serum levels of APP and Aβ1-42 in the observation group were lower than those in the control group (P < 0.05). Conclusion EA at Baihui (GV 20) and Fengfu (GV 6) combined with donepezil hydrochloride can effectively reduce the serum levels of APP and Aβ1-42 and improve the scores of MoCA and ADAS-Cog as well as the levels of N2 and P3 of P300 in AD patients. Such combination is superior to donepezil hydrochloride alone in improving the learning and memory abilities of AD patients.

【Keywords】 Alzheimer’s disease (AD); electroacupuncture (EA); governor vessel; Baihui (GV 20) point and Fengfu (GV 16) point; learning and memory ability; randomized controlled trial (RCT);

【DOI】

【Funds】 Natural Science Foundation of Heilongjiang Province for Joint Guide Project (LH2019H047) Scientific Research Project of Heilongjiang Provincial Health Commission (2019-192)

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This Article

ISSN:0255-2930

CN: 11-2024/R

Vol 40, No. 04, Pages 375-378

April 2020

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Article Outline

Abstract

  • 1 Clinical data
  • 2 Therapeutic methods
  • 3 Observation of curative effects
  • 4 Discussion
  • References