Correlation analysis of bacterial community and hypersaline environmental factors in extreme salt lakes on the Qinghai-Tibet Plateau
(2.Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China 810016)
【Abstract】The bacterial structure and diversity of extremely salty lakes on the Qinghai-Tibet Plateau were investigated using high-throughput sequencing approach based on 16S r RNA gene (V3–V4 region), and then the difference of community compositions and the restrict correlation of hypersaline environmental factors were investigated. Results showed that the bacterial communities of hypersaline samples were relatively stable and evolutionally convergent, and the complexity and the diversity index were significantly lower than the usual salt environment. The dominant bacterial phyla were affiliated to Firmicutes (74.04%–81.04% of relative abundance proportion), followed by Proteobacteria (15.51%–20.06%), Bacteroidetes (2.68%–4.84%) and Actinobacteria (0.71%–2.45%). Base on the comparative analysis of other types of hypersaline lakes, the characteristic genera in this study were Bacillus (50.63%–58.35%), Lactococcus (9.28%–11.81%) and Oceanobacillus (8.41%–10.52%). Canonical correspondence analysis showed that the dominant bacterial genera (including Bacillus, Lactococcus, Pseudomonas, Oceanobacillus, Stenotrophomonas, Psychrobacter, Myroides, Brochothrix and Arthrobacter) and the hypersaline samples cluster were positively correlated with the saline environment factors including total salinity, Cl−, K+, Mg2+, and CO32−.
【Keywords】 Qinghai-Tibet Plateau; extreme salt lake; halophilic bacteria; hypersaline environment; environmental factors;
(Translated by GAO YH)
 Oren A. Life at High Salt Concentrations [J]. Prokaryotes, 2006, 4 (5): 421–440.
 Maheshwari D K, SarafM. Halophiles [M]. Switzerland: Springer International Publishing, 2015: 355–357.
 Sorokin D Y, Berben T, Melton E D, et al. Microbial diversity and biogeochemical cycling in soda lakes [J]. Extremophiles Life Under Extreme Conditions, 2014, 18 (5): 791–809.
 Horikoshi K, Antranikian G, Bull A T, et al. Extremophiles Handbook [M]. Japan: Springer International Publishing, 2011: 256–400.
 Boutaiba S, Hacene H, Bidle K A, et al. Microbial diversity of the hypersaline Sidi Ameur and Himalatt Salt Lakes of the Algerian Sahara [J]. Journal of Arid Environments, 2011, 75 (10): 909–916.
 Kjeldsen K U, Loy A, Jakobsen T F, et al. Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment, Great Salt Lake (Utah) [J]. FEMS Microbiology Ecology, 2007, 60 (2): 287–298.
 Oren A, Baxter B K, Weimer B C. Microbial communities in salt lakes: phylogenetic diversity, metabolic diversity, and in situ activities [J]. Natural Resources & Environmental Issues, 2009, 15 (1): 1–7.
 Ventosa A, Oren A, Ma Y. Halophiles and Hypersaline Environments [M]. Berlin: Springer International Publishing, 2011: 48–49.
 Oren A. Halophilic archaea on Earth and in space: growth and survival under extreme conditions [J]. Philosophical Transactions of the Royal Society, 2014, 372 (2030): 1–12.
 Ghai R, PašićL, Fernández AB, et al. New abundant microbial groups in aquatic hypersaline environments [J]. Scientific Reports, 2011, 1 (9): 135–145.
 Ventosa A, Fernández A B, León M J, et al. The Santa Pola saltern as a model for studying the microbiota of hypersaline environments [J]. Extremophiles, 2014, 18 (5): 811–824.
 Liu Y, Priscu J C, Yao T, et al. A comparison of pelagic, littoral, and riverine bacterial assemblages in Lake Bangongco, Tibetan Plateau [J]. FEMS Microbiology Ecology, 2014, 89 (2): 1–11.
 Zhong Z P, Liu Y, Miao L L, et al. Prokaryotic community structure driven by salinity and ionic concentrations in plateau lakes of the Tibetan Plateau [J]. Applied & Environmental Microbiology, 2016, 82 (6): 1846–1858.
 Yun J, Ju Y, Deng Y, et al. Bacterial community structure in two permafrost wetlands on the Tibetan Plateau and Sanjiang Plain, China [J]. Microbial Ecology, 2014, 68 (2): 360–369.
 Xiong J, Liu Y, Lin X, et al. Geographic distance and p H drive bacterial distribution in alkaline lake sediments across Tibetan Plateau [J]. Environmental Microbiology, 2012, 14 (9): 2457–2466.
 Jiang H, Huang Q, Deng S, et al. Planktonic actinobacterial diversity along a salinity gradient of a river and five lakes on the Tibetan Plateau [J]. Extremophiles, 2010, 14 (4): 367–376.
 Hu A, Yao T, Jiao N, et al. Community structures of ammoniaoxidising archaea and bacteria in high-altitude lakes on the Tibetan Plateau [J]. Freshwater Biology, 2010, 55 (11): 2375–2390.
 Yu Y, Lee C, Kim J, et al. Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction [J]. Biotechnology & Bioengineering, 2005, 89 (6): 670–679.
 Demergasso C, Escudero L, Casamayor EO, et al. Novelty and spatio-temporal heterogeneity in the bacterial diversity of hypersaline Lake Tebenquiche (Salar de Atacama) [J]. Extremophiles, 2008, 12 (4): 491–504.
 Oren A. Molecular ecology of extremely halophilic Archaea and Bacteria [J]. FEMS Microbiology Ecology, 2002, 39 (1): 1–7.
 Simachew A, Lanzén A, Gessesse A, et al. Prokaryotic community diversity along an increasing salt gradient in a Soda Ash concentration pond [J]. Microbial Ecology, 2016, 71 (2): 326–338.
 Tazi L, Breakwell D P, Harker A R, et al. Life in extreme environments: microbial diversity in Great Salt Lake, Utah [J]. Extremophiles, 2014, 18 (3): 525–535.
 Ali I, Prasongsuk S, Akbar A, et al. Hypersaline habitats and halophilic microorganisms [J]. Maejo International Journal of Science and Technology, 2016, 10 (3): 330–345.
 Abdallah M B, Karray F, Mhiri N, et al. Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid [J]. Extremophiles, 2016, 20 (2): 125–138.
 Çınar S, Mutlu M B. Comparative analysis of prokaryotic diversity in solar salterns in eastern Anatolia (Turkey) [J]. Extremophiles, 2016, 20 (5): 589–601.
 Mesbah N M, Abou-El-Ela S H, Wiegel J. Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt [J]. Microbial Ecology, 2007, 54 (4): 598–617.
 Odamaki T, Yonezawa S, Kitahara M, et al. Novel multiplex polymerase chain reaction primer set for identification of Lactococcus species [J]. Letters in Applied Microbiology, 2011, 52 (5): 491–496.
 MarchéL, Saraoui T, Remenant B, et al. Complete genome sequence of Lactococcus piscium CNCM I-4031, a bioprotective strain for seafood products [J]. Genome Announcements, 2017, 5 (4): 1–2.
 Saraoui T, Leroi F, Björkroth J, et al. Lactococcus piscium: a psychrotrophic lactic acid bacterium with bioprotective or spoilage activity in food-a review [J]. Journal of Applied Microbiology, 2016, 121 (4): 907–918.
 Smith W M, Dykes G A, Soomro A H, et al. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology [M], Spain: Formatex Research Center Publisher, 2010: 1106–1118.
 Amoozegar M A, Bagheri M, Makhdoumi A, et al. Oceanobacillus halophilus sp. nov. a novel moderately halophilic bacterium from a hypersaline lake [J]. International Journal of Systematic & Evolutionary Microbiology, 2016, 66 (3): 1317–1322.
 Kim Y G, Choi D H, Hyun S, et al. Oceanobacillus profundus sp. nov. isolated from a deep-sea sediment core [J]. International Journal of Systematic & Evolutionary Microbiology, 2007, 57: 409–413.
 Yuan W, Su X, Cui G, et al. Microbial community structure in hypolentic zones of a brine lake in a desert plateau, China [J]. Environmental Earth Sciences, 2016, 75 (15): 1–14.