Correlation analysis of bacterial community and hypersaline environmental factors in extreme salt lakes on the Qinghai-Tibet Plateau

ZHU De-rui1 HAN Rui2 SHI Qing1 SHEN Guo-ping1 LONG Qi-fu1 SHUANG Jie1

(1.Research Center of Basic Medical Sciences, Qinghai University Medical College, Xining, China 810016)
(2.Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China 810016)

【Abstract】The bacterial structure and diversity of extremely salty lakes on the Qinghai-Tibet Plateau were investigated using high-throughput sequencing approach based on 16S r RNA gene (V3–V4 region), and then the difference of community compositions and the restrict correlation of hypersaline environmental factors were investigated. Results showed that the bacterial communities of hypersaline samples were relatively stable and evolutionally convergent, and the complexity and the diversity index were significantly lower than the usual salt environment. The dominant bacterial phyla were affiliated to Firmicutes (74.04%–81.04% of relative abundance proportion), followed by Proteobacteria (15.51%–20.06%), Bacteroidetes (2.68%–4.84%) and Actinobacteria (0.71%–2.45%). Base on the comparative analysis of other types of hypersaline lakes, the characteristic genera in this study were Bacillus (50.63%–58.35%), Lactococcus (9.28%–11.81%) and Oceanobacillus (8.41%–10.52%). Canonical correspondence analysis showed that the dominant bacterial genera (including Bacillus, Lactococcus, Pseudomonas, Oceanobacillus, Stenotrophomonas, Psychrobacter, Myroides, Brochothrix and Arthrobacter) and the hypersaline samples cluster were positively correlated with the saline environment factors including total salinity, Cl, K+, Mg2+, and CO32−.

【Keywords】 Qinghai-Tibet Plateau; extreme salt lake; halophilic bacteria; hypersaline environment; environmental factors;

【DOI】

【Funds】 National Natural Science Foundation of China (31560039) Natural Science Foundation of Qinghai Province (2015ZJY23, 2015ZJ929Q)

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(Translated by GAO YH)

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This Article

ISSN:1000-6923

CN: 11-2201/X

Vol 37, No. 12, Pages 4657-4666

December 2017

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Result and analysis
  • 3 Conclusion
  • References