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细菌对城市污水中斜生栅藻生长与产脂的影响

韩松芳1 金文标1 涂仁杰1 周旭1 陈洪一1

(1.哈尔滨工业大学深圳研究生院深圳微藻生物能源工程实验室, 广东深圳 518055)

【摘要】研究并分析了EM菌和LAS菌对斜生栅藻 (Scenedesmus obliquus) 生长和油脂积累的促进作用.结果表明:添加上述2种细菌对城市污水中斜生栅藻的干重和油脂产量有显著的促进作用, 其油脂产量分别提高了36.2%和21.5%.通过对生成的脂肪酸甲酯进行气相色谱分析, 结果显示EM菌的添加提升了斜生栅藻单不饱和脂肪酸的含量, 有利于提升所得生物柴油的质量.培养结束后污水的菌群结构分析显示投加细菌会增加污水中菌群的丰富度和多样性, 且相比于对照组, 加菌后的试验组中β-Proteobacteria、α-Proteobacteria丰度有所提高, 而拟杆菌门 (Bacteroidetes) 丰度有所降低.

【关键词】 斜生栅藻;城市污水;细菌;产脂;

【DOI】

【基金资助】 深圳市科技计划资助项目 (JCYJ20150529114024234) ;

Effects of bacteria on growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater

HAN Song-fang1 JIN Wen-biao1 TU Ren-jie1 ZHOU Xu1 CHEN Hong-yi1

(1.Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, China 518055)

【Abstract】In the study, EM bacteria and LAS bacteria were found to enhance the growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater. The results showed that the lipid production of S. obliquus was increased by 36.2% and 21.5% after adding the above-mentioned bacteria, respectively. According to the GC analysis of the lipids, EM bacteria could increase the content of monounsaturated fatty acid in S. obliquus, and thus improving the grade of biodiesel. The analysis of microbial community structure in municipal wastewater showed that the richness and diversity of bacteria in wastewater were increased significantly. In addition, the abundance of β-Proteobacteria and α-Proteobacteria was increased, while the abundance of Bacteroidetes was decreased.

【Keywords】 Scenedesmus obliquus; municipal wastewater; bacteria; lipid production;

【DOI】

【Funds】 Shenzhen Science and Technology Plan (JCYJ20150529114024234);

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This Article

ISSN:1000-6923

CN:11-2201/X

Vol 37, No. 10, Pages 3867-3872

October 2017

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and discussions
  • 3 Conclusions
  • References