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韩松芳1 金文标1 涂仁杰1 周旭1 陈洪一1

(1.哈尔滨工业大学深圳研究生院深圳微藻生物能源工程实验室, 广东深圳 518055)

【摘要】研究并分析了EM菌和LAS菌对斜生栅藻 (Scenedesmus obliquus) 生长和油脂积累的促进作用.结果表明:添加上述2种细菌对城市污水中斜生栅藻的干重和油脂产量有显著的促进作用, 其油脂产量分别提高了36.2%和21.5%.通过对生成的脂肪酸甲酯进行气相色谱分析, 结果显示EM菌的添加提升了斜生栅藻单不饱和脂肪酸的含量, 有利于提升所得生物柴油的质量.培养结束后污水的菌群结构分析显示投加细菌会增加污水中菌群的丰富度和多样性, 且相比于对照组, 加菌后的试验组中β-Proteobacteria、α-Proteobacteria丰度有所提高, 而拟杆菌门 (Bacteroidetes) 丰度有所降低.

【关键词】 斜生栅藻;城市污水;细菌;产脂;


【基金资助】 深圳市科技计划资助项目 (JCYJ20150529114024234) ;

Effects of bacteria on growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater

HAN Song-fang1 JIN Wen-biao1 TU Ren-jie1 ZHOU Xu1 CHEN Hong-yi1

(1.Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, China 518055)

【Abstract】In the study, EM bacteria and LAS bacteria were found to enhance the growth and lipid production of Scenedesmus obliquus cultivated in municipal wastewater. The results showed that the lipid production of S. obliquus was increased by 36.2% and 21.5% after adding the above-mentioned bacteria, respectively. According to the GC analysis of the lipids, EM bacteria could increase the content of monounsaturated fatty acid in S. obliquus, and thus improving the grade of biodiesel. The analysis of microbial community structure in municipal wastewater showed that the richness and diversity of bacteria in wastewater were increased significantly. In addition, the abundance of β-Proteobacteria and α-Proteobacteria was increased, while the abundance of Bacteroidetes was decreased.

【Keywords】 Scenedesmus obliquus; municipal wastewater; bacteria; lipid production;


【Funds】 Shenzhen Science and Technology Plan (JCYJ20150529114024234);

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    [1] Chisti Y. Constraints to commercialization of algal fuels [J]. Journal of Biotechnology, 2013, 167 (3): 201–214.

    [2] Han S F, Jin W B, Tu R J, et al. Biofuel production from microalgae as feedstock: current status and potential [J]. Critical Reviews in Biotechnology, 2015, 35 (2): 255–268.

    [3] Abomohra A E, Jin W B, Tu R J, et al. Microalgal biomass production as a sustainable feedstock for biodiesel: current status andperspectives [J]. Renewable & Sustainable Energy Reviews, 2016, 64 (64): 596–606.

    [4] Yang J, Xu M, Zhang M X, et al. Life-cycle analysis on biodiesel production from microalgae: Water footprint and nutrients balance [J]. Bioresource Technology, 2011, 102: 159–165.

    [5] Morales-Amaral M d M, Gómez-Serrano C, Acién F G, et al. Production of microalgae using centrate from anaerobic digestion as the nutrient source [J]. Algal Research, 2015, 9: 297–305.

    [6] Han S F, Jin W B, Tu R J, et al. Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater [J]. Bioprocess and Biosystems Engineering, 2016, 39 (7): 1073–1079.

    [7] Zhang Y L, Chu H Q, Zhou X F, et al. 废水微藻资源化处理原理与技术 [M]. Beijing: Science Press, 2015: 166–169 (in Chinese).

    [8] Wu X D, Ruan R S, Wang H, et al. Current status and prospect of sewage purification with the algal-microbe symbiotic system [J]. Environmental Engineering, 2014, (3): 34–37 (in Chinese).

    [9] Wang B, Zhou J T, Yang B L, et al. Deep treatment of wastewater by co-immobilized photosynthetic bacteria and algae [J]. Journal of Dalian Minzu University, 2014, 16 (3): 249–252 (in Chinese).

    [10] Gonzalez L E, Bashan Y. Increased growth of the microalgae Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense [J]. Applied and Environmental Microbiology, 2000, 66 (4): 1527–1531.

    [11] Mayali X, Doucette G J. Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis (Dinophyceae) [J]. Harmful Algae, 2002, 1 (3): 277–293.

    [12] Ramsundar P, Guldhe A, Singh P, et al. Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation [J]. Bioresource Technology, 2017, 227: 82–92.

    [13] Ryu B G, Kim E J, Kim H S, et al. Simultaneous treatment of municipal wastewater and biodiesel production by cultivation of Chlorella vulgaris with indigenous wastewater bacteria [J]. Biotechnology and Bioprocess Engineering, 2014, 19 (2): 201–210.

    [14] Abomohra A E, Wagner M, El-Sheekh M, et al. Lipid and total fatty acid productivity in photoautotrophic fresh water microalgae: screening studies towards biodiesel production [J]. Journal of Applied Phycology, 2013, 25 (4): 931–936.

    [15] Stainier R Y, Kunisawa R, Mandel M, et al. Purification and properties of unicellular blue-green algae (order Chroococcales) [J]. Bacteriology Reviews, 1971, 35 (2): 171–205.

    [16] Yan Y. Study on the distribution of nitrogen ceycling microbial community in bioremediation process of Buji River [D]. Harbin: Harbin Institute of Technology, 2008 (in Chinese).

    [17] Ding B B. Study on the construction of highly-efficient LAS-degrading bacteria in a bacteria-algae combined rotating biological contactor [D]. Harbin: Harbin Institute of Technology, 2010 (in Chinese).

    [18] Sun C F. Isolation and screening of dimethylformamide biodegrading bacteria and study on its degradation [D]. Harbin: Harbin Institute of Technology, 2010 (in Chinese).

    [19] Folch J, Lees M, Stanley G H S. A simple method for the isolation and purification of total lipids from animal tissues [J]. Journal of Biological Chemistry, 1957, 226: 497–509.

    [20] Kaczmarzyk D, Fulda M. Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling [J]. Plant Physiology, 2010, 152 (3): 1598–1610.

    [21] Liu L L, Huang X X, Wei L K, et al. Removal of nitrogen and phosphorus by 15 strains of microalgae and their nutritional values in piggery sewage [J]. Acta Scientiae Circumstantiae, 2014, 34 (8): 1986–1994 (in Chinese).

    [22] Vidyashankar S, Venu Gopal K S, Swarnalatha G V, et al. Characterization of fatty acids and hydrocarbons of chlorophycean microalgae towards their use as biofuel source [J]. Biomass & Bioenergy, 2015, 77: 75–91.

    [23] Song M, Pei H, Hu W, et al. Evaluation of the potential of10microalgal strains for biodiesel production [J]. Bioresource Technology, 2013, 141: 245–251.

    [24] Ma Y, Wang Z, Yu C, et al. Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production [J]. Bioresource Technology, 2014, 167: 503–509.

    [25] Stansell G R, Gray V M, Sym S D. Microalgal fatty acid composition:implications for biodiesel quality [J]. Journal of Applied Phycology, 2012, 24 (4): 791–801.

    [26] Hoekman S K, Broch A, Robbins C, et al. Review of biodiesel composition, properties, and specifications [J]. Renewable & Sustainable Energy Reviews, 2012, 16: 143–169.

    [27] Zhang T, Shao M F, Ye L. 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14sewage treatment plants [J]. ISME Journal, 2012, 6 (6): 1137–1147.

    [28] Shao K Q, Zhang L, Wang Y P, et al. The responses of the taxa composition of particle-attached bacterial community to the decomposition of Microcystis blooms [J]. Science of the Total Environment, 2014, 488–489: 236–242.

    [29] Ramanan R, Kang Z, Kim B, et al. Phycosphere bacterial diversity in green algae reveals an apparent similarity across habitats [J]. Algal Research, 2015, 8: 140–144.

    [30] Lee J, Cho D, Ramanan R, et al. Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris [J]. Bioresource Technology, 2013, 131: 195–201.

This Article



Vol 37, No. 10, Pages 3867-3872

October 2017


Article Outline


  • 1 Materials and methods
  • 2 Results and discussions
  • 3 Conclusions
  • References