Effect of solution environment on ultrafiltration separation of Panax notoginseng total saponins based on molecular state

LI Cun-yu1,2 ZHI Xing-lei1,2 NIU Xue-yu1 DAI Ling-jie1 LI Hong-yang1 PENG Guo-ping1,2

(1.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China 210023)
(2.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, China 210023)

【Abstract】 Objective To clarify the effect of solution environment on ultrafiltration separation of Panax notoginseng total saponins (PNS) based on the molecular state. Methods In the experiment, the transmittance and surface tension were selected as indexes for analyzing the effect of ethanol, inorganic salts, surfactants, and pH on the molecular state of PNS. Then, ethanol, NaCl, and pH were selected as influencing factors to analyze the separation rule of notoginsenoside R1 (R1) and ginsenoside Rb1 (Rb1). Results The intermolecular interaction of saponins was weakened by increasing the ethanol concentration. The pH value promoted saponin ionization, increased critical micelle concentration, and increased PNS ultrafiltration transmittance. The salting out effect of inorganic salt reduced the critical micelle concentration and PNS transmittance. The surfactant type was related to the ultrafiltration separation behavior of PNS. Rb1 was more sensitive to the factors than R1 by response surface methodology. Conclusion The effect of solution environmental factors on the ultrafiltration separation of PNS was clarified by the combination of single factor analysis and response surface methodology. The saponins can be separated purposefully by dynamically adjusting the molecular state.

【Keywords】 ultrafiltration; Panax notoginseng total saponins; solution environment; molecular state; notoginsenoside R1; ginsenoside Rb1;


【Funds】 National Natural Science Foundation of China (81603307) National Natural Science Foundation of Higher Education Institutions in Jiangsu Province (17KJB360010)

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This Article


CN: 12-1108/R

Vol 50, No. 21, Pages 5246-5252

November 2019


Article Outline


  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Discussion
  • References